Reviewed, UniProtKB/Swiss-Prot P0AC53 (G6PD_ECOLI)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	zwf
Ordered Locus Names:	b1852, JW1841

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-6-phosphate dehydrogenase activity Inferred from mutant phenotype. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaN	P0A988	1	EBI-555656,EBI-542385

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068118
Sites
	Active site	239	1	Proton acceptor By similarity
	Binding site	18	1	NADP By similarity
	Binding site	50	1	NADP By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	344	1	Substrate By similarity
Natural variations
	Natural variant	100	1	S → N in strain: ECOR 4 and ECOR 10.
Experimental info
	Sequence conflict	268 – 293	26	LKSLR…TAGFA → PEVSSPHRPLQRTRKNRTRA IYCVP in AAA24775. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AC53-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 263F07D298EAFCD3	FASTA	491	55,704
	10 20 30 40 50 60 MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK 70 80 90 100 110 120 VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP 130 140 150 160 170 180 PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG 190 200 210 220 230 240 KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL 250 260 270 280 290 300 LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG 310 320 330 340 350 360 YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF 370 380 390 400 410 420 KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA 430 440 450 460 470 480 YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI 490 TRDGRSWNEF E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase." Rowley D.L., Wolf R.E. Jr. J. Bacteriol. 173:968-977(1991) [PubMed: 1704005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Clonal divergence in Escherichia coli as a result of recombination, not mutation." Guttman D.S., Dykhuizen D.E. Science 266:1380-1383(1994) [PubMed: 7973728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-368. Strain: Various ECOR strains.
[6]	"Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway." Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E. Gene 130:155-156(1993) [PubMed: 8344525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 321-491. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55005 Genomic DNA. Translation: AAA24775.1. U00096 Genomic DNA. Translation: AAC74922.1. AP009048 Genomic DNA. Translation: BAA15660.1. U13783 Genomic DNA. Translation: AAA57018.1. U13784 Genomic DNA. Translation: AAA57019.1. U13785 Genomic DNA. Translation: AAA57020.1. U13786 Genomic DNA. Translation: AAA57021.1. U13787 Genomic DNA. Translation: AAA57022.1. U13788 Genomic DNA. Translation: AAA57023.1. U13789 Genomic DNA. Translation: AAA57024.1. U13790 Genomic DNA. Translation: AAA57025.1. U13791 Genomic DNA. Translation: AAA57026.1. U13792 Genomic DNA. Translation: AAA57027.1. U13793 Genomic DNA. Translation: AAA57028.1. U13794 Genomic DNA. Translation: AAA57029.1. X63694 Genomic DNA. Translation: CAA45220.1.
PIR	D64947.
RefSeq	AP_002472.1. NP_416366.1.
3D structure databases
SMR	P0AC53. Positions 5-488.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AC53. 46 interactions.
STRING	P0AC53.
2-D gel databases
SWISS-2DPAGE	P0AC53.
ECO2DBASE	F048.8. 6TH EDITION.
Genome annotation databases
GeneID	946370.
GenomeReviews	Gene locus JW1841 in contig AP009048_GR. Gene locus b1852 in contig U00096_GR.
KEGG	ecj:JW1841. eco:b1852.
Organism-specific databases
EchoBASE	EB1203.
EcoGene	EG11221. zwf.
CMR	Search...
Phylogenomic databases
eggNOG	COG0364.
HOGENOM	HBG322449.
OMA	VVRGNAT.
Enzyme and pathway databases
BioCyc	EcoCyc:GLU6PDEHYDROG-MONOMER. ECOL168927:B1852-MONOMER. MetaCyc:GLU6PDEHYDROG-MONOMER.
Gene expression databases
Genevestigator	P0AC53.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_ECOLI

Accession

Primary (citable) accession number: P0AC53
Secondary accession number(s): P22992 , P78069, Q60134, Q60139

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)