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P0AC53 (G6PD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase

Short name=G6PD
EC=1.1.1.49

Cleaved into the following chain:

  1. Extracellular death factor
    Short name=EDF
Gene names
Name:zwf
Ordered Locus Names:b1852, JW1841
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Ref.8 Ref.9

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. Ref.8

Post-translational modification

Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn Probable. HAMAP-Rule MF_00966

Disruption phenotype

Loss of production of extracellular death factor. Ref.9

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Caution

Strain K12 / MG1655 is deficient in both production and response to EDF, unlike strains K12 / MC4100, K12 / W3110 and K12 / K38, all of which make and respond to EDF (Ref.10).

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-555656,EBI-555656

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Glucose-6-phosphate 1-dehydrogenase HAMAP-Rule MF_00966
PRO_0000068118
Chain199 – 2035Extracellular death factor HAMAP-Rule MF_00966
PRO_0000404298

Regions

Nucleotide binding92 – 932NADP By similarity

Sites

Active site2391Proton acceptor By similarity
Binding site501NADP By similarity
Binding site1471NADP By similarity
Binding site1771Substrate By similarity
Binding site1811Substrate By similarity
Binding site2151Substrate By similarity
Binding site2341Substrate By similarity
Binding site3391Substrate By similarity
Binding site3441Substrate By similarity

Natural variations

Natural variant1001S → N in strain: ECOR 4 and ECOR 10.

Experimental info

Sequence conflict268 – 29326LKSLR…TAGFA → PEVSSPHRPLQRTRKNRTRA IYCVP in AAA24775. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0AC53 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 263F07D298EAFCD3

FASTA49155,704
        10         20         30         40         50         60 
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK 

        70         80         90        100        110        120 
VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP 

       130        140        150        160        170        180 
PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG 

       190        200        210        220        230        240 
KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL 

       250        260        270        280        290        300 
LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG 

       310        320        330        340        350        360 
YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF 

       370        380        390        400        410        420 
KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA 

       430        440        450        460        470        480 
YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI 

       490 
TRDGRSWNEF E 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase."
Rowley D.L., Wolf R.E. Jr.
J. Bacteriol. 173:968-977(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Clonal divergence in Escherichia coli as a result of recombination, not mutation."
Guttman D.S., Dykhuizen D.E.
Science 266:1380-1383(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-368.
Strain: Various ECOR strains.
[6]"Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 321-491.
Strain: K12.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[8]"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate."
Zhao J., Baba T., Mori H., Shimizu K.
Metab. Eng. 6:164-174(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PATHWAY.
Strain: K12 / BW25113.
[9]"A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli."
Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H.
Science 318:652-655(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS EXTRACELLULAR DEATH FACTOR (EDF), POSSIBLE PROTEOLYTIC PROCESSING, DISRUPTION PHENOTYPE.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[10]"The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli."
Kolodkin-Gal I., Engelberg-Kulka H.
J. Bacteriol. 190:3169-3175(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: EDF PRODUCTION, STRAIN DIFFERENCES IN ABILITY TO MAKE AND RESPOND TO EDF.
Strain: K12 / K38 / S26, K12 / MC4100 / ATCC 35695 / DSM 6574, K12 / MG1655 / ATCC 47076 and K12 / W3110.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55005 Genomic DNA. Translation: AAA24775.1.
U00096 Genomic DNA. Translation: AAC74922.1.
AP009048 Genomic DNA. Translation: BAA15660.1.
U13783 Genomic DNA. Translation: AAA57018.1.
U13784 Genomic DNA. Translation: AAA57019.1.
U13785 Genomic DNA. Translation: AAA57020.1.
U13786 Genomic DNA. Translation: AAA57021.1.
U13787 Genomic DNA. Translation: AAA57022.1.
U13788 Genomic DNA. Translation: AAA57023.1.
U13789 Genomic DNA. Translation: AAA57024.1.
U13790 Genomic DNA. Translation: AAA57025.1.
U13791 Genomic DNA. Translation: AAA57026.1.
U13792 Genomic DNA. Translation: AAA57027.1.
U13793 Genomic DNA. Translation: AAA57028.1.
U13794 Genomic DNA. Translation: AAA57029.1.
X63694 Genomic DNA. Translation: CAA45220.1.
PIRD64947.
RefSeqNP_416366.1. NC_000913.3.
YP_490114.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AC53.
SMRP0AC53. Positions 12-480.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35780N.
IntActP0AC53. 47 interactions.
MINTMINT-1317139.
STRING511145.b1852.

2D gel databases

SWISS-2DPAGEP0AC53.

Proteomic databases

PaxDbP0AC53.
PRIDEP0AC53.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74922; AAC74922; b1852.
BAA15660; BAA15660; BAA15660.
GeneID12930160.
946370.
KEGGecj:Y75_p1828.
eco:b1852.
PATRIC32119025. VBIEscCol129921_1930.

Organism-specific databases

EchoBASEEB1203.
EcoGeneEG11221. zwf.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046191.
KOK00036.
OMADSIMEAW.
OrthoDBEOG61308Z.
PhylomeDBP0AC53.

Enzyme and pathway databases

BioCycEcoCyc:GLU6PDEHYDROG-MONOMER.
ECOL316407:JW1841-MONOMER.
MetaCyc:GLU6PDEHYDROG-MONOMER.
UniPathwayUPA00115; UER00408.

Gene expression databases

GenevestigatorP0AC53.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AC53.

Entry information

Entry nameG6PD_ECOLI
AccessionPrimary (citable) accession number: P0AC53
Secondary accession number(s): P22992 expand/collapse secondary AC list , P78069, Q60134, Q60139
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene