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P0AC53

- G6PD_ECOLI

UniProt

P0AC53 - G6PD_ECOLI

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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.2 PublicationsUniRule annotation

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501NADPUniRule annotation
Binding sitei147 – 1471NADPUniRule annotation
Binding sitei177 – 1771SubstrateUniRule annotation
Binding sitei181 – 1811SubstrateUniRule annotation
Binding sitei215 – 2151SubstrateUniRule annotation
Binding sitei234 – 2341SubstrateUniRule annotation
Active sitei239 – 2391Proton acceptorUniRule annotation
Binding sitei339 – 3391SubstrateUniRule annotation
Binding sitei344 – 3441SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 932NADPUniRule annotation

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: EcoliWiki
  2. identical protein binding Source: IntAct
  3. NADP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glucose metabolic process Source: EcoCyc
  2. pentose-phosphate shunt Source: EcoCyc
  3. quorum sensing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Quorum sensing

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:GLU6PDEHYDROG-MONOMER.
ECOL316407:JW1841-MONOMER.
MetaCyc:GLU6PDEHYDROG-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
Short name:
G6PDUniRule annotation
Cleaved into the following chain:
Gene namesi
Name:zwfUniRule annotation
Ordered Locus Names:b1852, JW1841
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11221. zwf.

Pathology & Biotechi

Disruption phenotypei

Loss of production of extracellular death factor.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Glucose-6-phosphate 1-dehydrogenasePRO_0000068118Add
BLAST
Chaini199 – 2035Extracellular death factorPRO_0000404298

Post-translational modificationi

Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn (Probable).Curated

Proteomic databases

PaxDbiP0AC53.
PRIDEiP0AC53.

2D gel databases

SWISS-2DPAGEP0AC53.

Expressioni

Gene expression databases

GenevestigatoriP0AC53.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-555656,EBI-555656

Protein-protein interaction databases

DIPiDIP-35780N.
IntActiP0AC53. 47 interactions.
MINTiMINT-1317139.
STRINGi511145.b1852.

Structurei

3D structure databases

ProteinModelPortaliP0AC53.
SMRiP0AC53. Positions 12-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
InParanoidiP0AC53.
KOiK00036.
OMAiDSIMEAW.
OrthoDBiEOG61308Z.
PhylomeDBiP0AC53.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC53-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR
60 70 80 90 100
ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS
110 120 130 140 150
RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG
160 170 180 190 200
TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN
210 220 230 240 250
WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS
260 270 280 290 300
PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG
310 320 330 340 350
YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV
360 370 380 390 400
YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ
410 420 430 440 450
ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD
460 470 480 490
SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Length:491
Mass (Da):55,704
Last modified:November 8, 2005 - v1
Checksum:i263F07D298EAFCD3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 29326LKSLR…TAGFA → PEVSSPHRPLQRTRKNRTRA IYCVP in AAA24775. (PubMed:1704005)CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti100 – 1001S → N in strain: ECOR 4 and ECOR 10.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55005 Genomic DNA. Translation: AAA24775.1.
U00096 Genomic DNA. Translation: AAC74922.1.
AP009048 Genomic DNA. Translation: BAA15660.1.
U13783 Genomic DNA. Translation: AAA57018.1.
U13784 Genomic DNA. Translation: AAA57019.1.
U13785 Genomic DNA. Translation: AAA57020.1.
U13786 Genomic DNA. Translation: AAA57021.1.
U13787 Genomic DNA. Translation: AAA57022.1.
U13788 Genomic DNA. Translation: AAA57023.1.
U13789 Genomic DNA. Translation: AAA57024.1.
U13790 Genomic DNA. Translation: AAA57025.1.
U13791 Genomic DNA. Translation: AAA57026.1.
U13792 Genomic DNA. Translation: AAA57027.1.
U13793 Genomic DNA. Translation: AAA57028.1.
U13794 Genomic DNA. Translation: AAA57029.1.
X63694 Genomic DNA. Translation: CAA45220.1.
PIRiD64947.
RefSeqiNP_416366.1. NC_000913.3.
YP_490114.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74922; AAC74922; b1852.
BAA15660; BAA15660; BAA15660.
GeneIDi12930160.
946370.
KEGGiecj:Y75_p1828.
eco:b1852.
PATRICi32119025. VBIEscCol129921_1930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55005 Genomic DNA. Translation: AAA24775.1 .
U00096 Genomic DNA. Translation: AAC74922.1 .
AP009048 Genomic DNA. Translation: BAA15660.1 .
U13783 Genomic DNA. Translation: AAA57018.1 .
U13784 Genomic DNA. Translation: AAA57019.1 .
U13785 Genomic DNA. Translation: AAA57020.1 .
U13786 Genomic DNA. Translation: AAA57021.1 .
U13787 Genomic DNA. Translation: AAA57022.1 .
U13788 Genomic DNA. Translation: AAA57023.1 .
U13789 Genomic DNA. Translation: AAA57024.1 .
U13790 Genomic DNA. Translation: AAA57025.1 .
U13791 Genomic DNA. Translation: AAA57026.1 .
U13792 Genomic DNA. Translation: AAA57027.1 .
U13793 Genomic DNA. Translation: AAA57028.1 .
U13794 Genomic DNA. Translation: AAA57029.1 .
X63694 Genomic DNA. Translation: CAA45220.1 .
PIRi D64947.
RefSeqi NP_416366.1. NC_000913.3.
YP_490114.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AC53.
SMRi P0AC53. Positions 12-480.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35780N.
IntActi P0AC53. 47 interactions.
MINTi MINT-1317139.
STRINGi 511145.b1852.

2D gel databases

SWISS-2DPAGE P0AC53.

Proteomic databases

PaxDbi P0AC53.
PRIDEi P0AC53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74922 ; AAC74922 ; b1852 .
BAA15660 ; BAA15660 ; BAA15660 .
GeneIDi 12930160.
946370.
KEGGi ecj:Y75_p1828.
eco:b1852.
PATRICi 32119025. VBIEscCol129921_1930.

Organism-specific databases

EchoBASEi EB1203.
EcoGenei EG11221. zwf.

Phylogenomic databases

eggNOGi COG0364.
HOGENOMi HOG000046191.
InParanoidi P0AC53.
KOi K00036.
OMAi DSIMEAW.
OrthoDBi EOG61308Z.
PhylomeDBi P0AC53.

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .
BioCyci EcoCyc:GLU6PDEHYDROG-MONOMER.
ECOL316407:JW1841-MONOMER.
MetaCyc:GLU6PDEHYDROG-MONOMER.

Miscellaneous databases

PROi P0AC53.

Gene expression databases

Genevestigatori P0AC53.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000110. G6PD. 1 hit.
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase."
    Rowley D.L., Wolf R.E. Jr.
    J. Bacteriol. 173:968-977(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Clonal divergence in Escherichia coli as a result of recombination, not mutation."
    Guttman D.S., Dykhuizen D.E.
    Science 266:1380-1383(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-368.
    Strain: Various ECOR strains.
  6. "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
    Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
    Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 321-491.
    Strain: K12.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  8. "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate."
    Zhao J., Baba T., Mori H., Shimizu K.
    Metab. Eng. 6:164-174(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PATHWAY.
    Strain: K12 / BW25113.
  9. "A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli."
    Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H.
    Science 318:652-655(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS EXTRACELLULAR DEATH FACTOR (EDF), POSSIBLE PROTEOLYTIC PROCESSING, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  10. "The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli."
    Kolodkin-Gal I., Engelberg-Kulka H.
    J. Bacteriol. 190:3169-3175(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: EDF PRODUCTION, STRAIN DIFFERENCES IN ABILITY TO MAKE AND RESPOND TO EDF.
    Strain: K12 / K38 / S26, K12 / MC4100 / ATCC 35695 / DSM 6574, K12 / MG1655 / ATCC 47076 and K12 / W3110.

Entry informationi

Entry nameiG6PD_ECOLI
AccessioniPrimary (citable) accession number: P0AC53
Secondary accession number(s): P22992
, P78069, Q60134, Q60139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain K12 / MG1655 is deficient in both production and response to EDF, unlike strains K12 / MC4100, K12 / W3110 and K12 / K38, all of which make and respond to EDF.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3