Glucose-6-phosphate 1-dehydrogenase - Escherichia coli (strain K12)

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P0AC53 (G6PD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate 1-dehydrogenase
Short name=G6PD
EC=1.1.1.49

Cleaved into the following chain:

Extracellular death factor
Short name=EDF

Gene names

Name:	zwf
Ordered Locus Names:	b1852, JW1841

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP⁺ = 6-phospho-D-glucono-1,5-lactone + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Post-translational modification	Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn Probable.
Disruption phenotype	Loss of production of extracellular death factor. Ref.7
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Caution	Strain K12 / MG1655 is deficient in both production and response to EDF, unlike strains K12 / MC4100, K12 / W3110 and K12 / K38, all of which make and respond to EDF.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Quorum sensing
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from mutant phenotype. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaN	P0A988	1	EBI-555656,EBI-542385

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

Glucose-6-phosphate 1-dehydrogenase

PRO_0000068118

Chain

199 – 203

Extracellular death factor

PRO_0000404298

Sites

Active site

239

Proton acceptor By similarity

Binding site

NADP By similarity

Binding site

NADP By similarity

Binding site

177

Substrate By similarity

Binding site

181

Substrate By similarity

Binding site

344

Substrate By similarity

Natural variations

Natural variant

100

S → N in strain: ECOR 4 and ECOR 10.

Experimental info

Sequence conflict

268 – 293

LKSLR…TAGFA → PEVSSPHRPLQRTRKNRTRA IYCVP in AAA24775. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P0AC53 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 263F07D298EAFCD3
Show »

FASTA

491

55,704

        10         20         30         40         50         60 
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK 

        70         80         90        100        110        120 
VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP 

       130        140        150        160        170        180 
PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG 

       190        200        210        220        230        240 
KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL 

       250        260        270        280        290        300 
LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG 

       310        320        330        340        350        360 
YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF 

       370        380        390        400        410        420 
KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA 

       430        440        450        460        470        480 
YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI 

       490 
TRDGRSWNEF E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase." Rowley D.L., Wolf R.E. Jr. J. Bacteriol. 173:968-977(1991) [PubMed: 1704005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Clonal divergence in Escherichia coli as a result of recombination, not mutation." Guttman D.S., Dykhuizen D.E. Science 266:1380-1383(1994) [PubMed: 7973728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-368. Strain: Various ECOR strains.
[6]	"Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway." Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E. Gene 130:155-156(1993) [PubMed: 8344525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 321-491. Strain: K12.
[7]	"A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli." Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H. Science 318:652-655(2007) [PubMed: 17962566] [Abstract] Cited for: FUNCTION AS EXTRACELLULAR DEATH FACTOR (EDF), POSSIBLE PROTEOLYTIC PROCESSING, DISRUPTION PHENOTYPE. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli." Kolodkin-Gal I., Engelberg-Kulka H. J. Bacteriol. 190:3169-3175(2008) [PubMed: 18310334] [Abstract] Cited for: EDF PRODUCTION, STRAIN DIFFERENCES IN ABILITY TO MAKE AND RESPOND TO EDF. Strain: K12 / K38 / S26, K12 / MC4100 / ATCC 35695 / DSM 6574, K12 / MG1655 / ATCC 47076 and K12 / W3110.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55005 Genomic DNA. Translation: AAA24775.1. U00096 Genomic DNA. Translation: AAC74922.1. AP009048 Genomic DNA. Translation: BAA15660.1. U13783 Genomic DNA. Translation: AAA57018.1. U13784 Genomic DNA. Translation: AAA57019.1. U13785 Genomic DNA. Translation: AAA57020.1. U13786 Genomic DNA. Translation: AAA57021.1. U13787 Genomic DNA. Translation: AAA57022.1. U13788 Genomic DNA. Translation: AAA57023.1. U13789 Genomic DNA. Translation: AAA57024.1. U13790 Genomic DNA. Translation: AAA57025.1. U13791 Genomic DNA. Translation: AAA57026.1. U13792 Genomic DNA. Translation: AAA57027.1. U13793 Genomic DNA. Translation: AAA57028.1. U13794 Genomic DNA. Translation: AAA57029.1. X63694 Genomic DNA. Translation: CAA45220.1.
PIR	D64947.
RefSeq	NP_416366.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AC53.
SMR	P0AC53. Positions 8-489.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35780N.
IntAct	P0AC53. 46 interactions.
MINT	MINT-1317139.
2D gel databases
SWISS-2DPAGE	P0AC53.
ECO2DBASE	F048.8. 6TH EDITION.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004007; EBESCP00000004007; EBESCG00000003276. EBESCT00000017983; EBESCP00000017274; EBESCG00000017039.
GeneID	946370.
GenomeReviews	Gene locus JW1841 in contig AP009048_GR. Gene locus b1852 in contig U00096_GR.
KEGG	ecj:JW1841. eco:b1852.
PATRIC	32119025. VBIEscCol129921_1930.
Organism-specific databases
EchoBASE	EB1203.
EcoGene	EG11221. zwf.
Phylogenomic databases
eggNOG	COG0364.
GeneTree	EBGT00050000010896.
HOGENOM	HBG322449.
OMA	RVTINYF.
PhylomeDB	P0AC53.
ProtClustDB	PRK05722.
Enzyme and pathway databases
BioCyc	EcoCyc:GLU6PDEHYDROG-MONOMER. MetaCyc:GLU6PDEHYDROG-MONOMER.
Gene expression databases
Genevestigator	P0AC53.
Family and domain databases
InterPro	IPR001282. G6P_DH. IPR019796. G6P_DH_AS. IPR022675. G6P_DH_C. IPR022674. G6P_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00036.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. Zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_ECOLI

Accession

Primary (citable) accession number: P0AC53
Secondary accession number(s): P22992

Q60139

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents