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P0AC53

- G6PD_ECOLI

UniProt

P0AC53 - G6PD_ECOLI

Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.2 PublicationsUniRule annotation

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501NADPUniRule annotation
    Binding sitei147 – 1471NADPUniRule annotation
    Binding sitei177 – 1771SubstrateUniRule annotation
    Binding sitei181 – 1811SubstrateUniRule annotation
    Binding sitei215 – 2151SubstrateUniRule annotation
    Binding sitei234 – 2341SubstrateUniRule annotation
    Active sitei239 – 2391Proton acceptorUniRule annotation
    Binding sitei339 – 3391SubstrateUniRule annotation
    Binding sitei344 – 3441SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi92 – 932NADPUniRule annotation

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: EcoliWiki
    2. identical protein binding Source: IntAct
    3. NADP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glucose metabolic process Source: EcoCyc
    2. pentose-phosphate shunt Source: EcoCyc
    3. quorum sensing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Quorum sensing

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:GLU6PDEHYDROG-MONOMER.
    ECOL316407:JW1841-MONOMER.
    MetaCyc:GLU6PDEHYDROG-MONOMER.
    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
    Short name:
    G6PDUniRule annotation
    Cleaved into the following chain:
    Gene namesi
    Name:zwfUniRule annotation
    Ordered Locus Names:b1852, JW1841
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11221. zwf.

    Pathology & Biotechi

    Disruption phenotypei

    Loss of production of extracellular death factor.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Glucose-6-phosphate 1-dehydrogenasePRO_0000068118Add
    BLAST
    Chaini199 – 2035Extracellular death factorPRO_0000404298

    Post-translational modificationi

    Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn Probable.Curated

    Proteomic databases

    PaxDbiP0AC53.
    PRIDEiP0AC53.

    2D gel databases

    SWISS-2DPAGEP0AC53.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AC53.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-555656,EBI-555656

    Protein-protein interaction databases

    DIPiDIP-35780N.
    IntActiP0AC53. 47 interactions.
    MINTiMINT-1317139.
    STRINGi511145.b1852.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC53.
    SMRiP0AC53. Positions 12-480.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046191.
    KOiK00036.
    OMAiDSIMEAW.
    OrthoDBiEOG61308Z.
    PhylomeDBiP0AC53.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AC53-1 [UniParc]FASTAAdd to Basket

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    MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR    50
    ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS 100
    RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG 150
    TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN 200
    WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS 250
    PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG 300
    YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV 350
    YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ 400
    ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD 450
    SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E 491
    Length:491
    Mass (Da):55,704
    Last modified:November 8, 2005 - v1
    Checksum:i263F07D298EAFCD3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti268 – 29326LKSLR…TAGFA → PEVSSPHRPLQRTRKNRTRA IYCVP in AAA24775. (PubMed:1704005)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti100 – 1001S → N in strain: ECOR 4 and ECOR 10.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55005 Genomic DNA. Translation: AAA24775.1.
    U00096 Genomic DNA. Translation: AAC74922.1.
    AP009048 Genomic DNA. Translation: BAA15660.1.
    U13783 Genomic DNA. Translation: AAA57018.1.
    U13784 Genomic DNA. Translation: AAA57019.1.
    U13785 Genomic DNA. Translation: AAA57020.1.
    U13786 Genomic DNA. Translation: AAA57021.1.
    U13787 Genomic DNA. Translation: AAA57022.1.
    U13788 Genomic DNA. Translation: AAA57023.1.
    U13789 Genomic DNA. Translation: AAA57024.1.
    U13790 Genomic DNA. Translation: AAA57025.1.
    U13791 Genomic DNA. Translation: AAA57026.1.
    U13792 Genomic DNA. Translation: AAA57027.1.
    U13793 Genomic DNA. Translation: AAA57028.1.
    U13794 Genomic DNA. Translation: AAA57029.1.
    X63694 Genomic DNA. Translation: CAA45220.1.
    PIRiD64947.
    RefSeqiNP_416366.1. NC_000913.3.
    YP_490114.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74922; AAC74922; b1852.
    BAA15660; BAA15660; BAA15660.
    GeneIDi12930160.
    946370.
    KEGGiecj:Y75_p1828.
    eco:b1852.
    PATRICi32119025. VBIEscCol129921_1930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55005 Genomic DNA. Translation: AAA24775.1 .
    U00096 Genomic DNA. Translation: AAC74922.1 .
    AP009048 Genomic DNA. Translation: BAA15660.1 .
    U13783 Genomic DNA. Translation: AAA57018.1 .
    U13784 Genomic DNA. Translation: AAA57019.1 .
    U13785 Genomic DNA. Translation: AAA57020.1 .
    U13786 Genomic DNA. Translation: AAA57021.1 .
    U13787 Genomic DNA. Translation: AAA57022.1 .
    U13788 Genomic DNA. Translation: AAA57023.1 .
    U13789 Genomic DNA. Translation: AAA57024.1 .
    U13790 Genomic DNA. Translation: AAA57025.1 .
    U13791 Genomic DNA. Translation: AAA57026.1 .
    U13792 Genomic DNA. Translation: AAA57027.1 .
    U13793 Genomic DNA. Translation: AAA57028.1 .
    U13794 Genomic DNA. Translation: AAA57029.1 .
    X63694 Genomic DNA. Translation: CAA45220.1 .
    PIRi D64947.
    RefSeqi NP_416366.1. NC_000913.3.
    YP_490114.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AC53.
    SMRi P0AC53. Positions 12-480.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35780N.
    IntActi P0AC53. 47 interactions.
    MINTi MINT-1317139.
    STRINGi 511145.b1852.

    2D gel databases

    SWISS-2DPAGE P0AC53.

    Proteomic databases

    PaxDbi P0AC53.
    PRIDEi P0AC53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74922 ; AAC74922 ; b1852 .
    BAA15660 ; BAA15660 ; BAA15660 .
    GeneIDi 12930160.
    946370.
    KEGGi ecj:Y75_p1828.
    eco:b1852.
    PATRICi 32119025. VBIEscCol129921_1930.

    Organism-specific databases

    EchoBASEi EB1203.
    EcoGenei EG11221. zwf.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046191.
    KOi K00036.
    OMAi DSIMEAW.
    OrthoDBi EOG61308Z.
    PhylomeDBi P0AC53.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .
    BioCyci EcoCyc:GLU6PDEHYDROG-MONOMER.
    ECOL316407:JW1841-MONOMER.
    MetaCyc:GLU6PDEHYDROG-MONOMER.

    Miscellaneous databases

    PROi P0AC53.

    Gene expression databases

    Genevestigatori P0AC53.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase."
      Rowley D.L., Wolf R.E. Jr.
      J. Bacteriol. 173:968-977(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Clonal divergence in Escherichia coli as a result of recombination, not mutation."
      Guttman D.S., Dykhuizen D.E.
      Science 266:1380-1383(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-368.
      Strain: Various ECOR strains.
    6. "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
      Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
      Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 321-491.
      Strain: K12.
    7. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    8. "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate."
      Zhao J., Baba T., Mori H., Shimizu K.
      Metab. Eng. 6:164-174(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PATHWAY.
      Strain: K12 / BW25113.
    9. "A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli."
      Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H.
      Science 318:652-655(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS EXTRACELLULAR DEATH FACTOR (EDF), POSSIBLE PROTEOLYTIC PROCESSING, DISRUPTION PHENOTYPE.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    10. "The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli."
      Kolodkin-Gal I., Engelberg-Kulka H.
      J. Bacteriol. 190:3169-3175(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: EDF PRODUCTION, STRAIN DIFFERENCES IN ABILITY TO MAKE AND RESPOND TO EDF.
      Strain: K12 / K38 / S26, K12 / MC4100 / ATCC 35695 / DSM 6574, K12 / MG1655 / ATCC 47076 and K12 / W3110.

    Entry informationi

    Entry nameiG6PD_ECOLI
    AccessioniPrimary (citable) accession number: P0AC53
    Secondary accession number(s): P22992
    , P78069, Q60134, Q60139
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Strain K12 / MG1655 is deficient in both production and response to EDF, unlike strains K12 / MC4100, K12 / W3110 and K12 / K38, all of which make and respond to EDF.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3