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Protein

Fumarate reductase iron-sulfur subunit

Gene

frdB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi63Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi66Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi78Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi149Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi205Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi211Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi215Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport, Tricarboxylic acid cycle
Ligand2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate reductase iron-sulfur subunit (EC:1.3.5.1)
Gene namesi
Name:frdB
Ordered Locus Names:Z5760, ECs5134
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001586992 – 244Fumarate reductase iron-sulfur subunitAdd BLAST243

Proteomic databases

PRIDEiP0AC49

Interactioni

Subunit structurei

Fumarate dehydrogenase forms part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, and two hydrophobic anchor proteins.By similarity

Protein-protein interaction databases

STRINGi155864.Z5760

Structurei

3D structure databases

ProteinModelPortaliP0AC49
SMRiP0AC49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 972Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST82
Domaini140 – 1694Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105E33 Bacteria
COG0479 LUCA
HOGENOMiHOG000160590
KOiK00245
OMAiIRDHEEN

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di1.10.1060.10, 1 hit
3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR012675 Beta-grasp_dom_sf
IPR009051 Helical_ferredxn
IPR004489 Succ_DH/fum_Rdtase_Fe-S
IPR025192 Succ_DH/fum_Rdtase_N
PfamiView protein in Pfam
PF13085 Fer2_3, 1 hit
PF13183 Fer4_8, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR00384 dhsB, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEMKNLKIE VVRYNPEVDT APHSAFYEVP YDATTSLLDA LGYIKDNLAP
60 70 80 90 100
DLSYRWSCRM AICGSCGMMV NNVPKLACKT FLRDYTDGMK VEALANFPIE
110 120 130 140 150
RDLVVDMTHF IESLEAIKPY IIGNSRTADQ GTNIQTPAQM AKYHQFSGCI
160 170 180 190 200
NCGLCYAACP QFGLNPEFIG PAAITLAHRY NEDSRDHGKK ERMAQLNSQN
210 220 230 240
GVWSCTFVGY CSEVCPKHVD PAAAIQQGKV ESSKDFLIAT LKPR
Length:244
Mass (Da):27,123
Last modified:January 23, 2007 - v2
Checksum:iAC1D7A73244D7AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG59354.1
BA000007 Genomic DNA Translation: BAB38557.1
PIRiF86111
F91270
RefSeqiNP_313161.1, NC_002695.1
WP_000829498.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG59354; AAG59354; Z5760
BAB38557; BAB38557; BAB38557
GeneIDi914097
KEGGiece:Z5760
ecs:ECs5134
PATRICifig|386585.9.peg.5367

Similar proteinsi

Entry informationi

Entry nameiFRDB_ECO57
AccessioniPrimary (citable) accession number: P0AC49
Secondary accession number(s): P00364
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health