P0AC47P00364Q2M6E9FRDB_ECOLIFumarate reductase iron-sulfur subunit1.3.5.1Quinol-fumarate reductase iron-sulfur subunitQFR iron-sulfur subunitfrdBb4153JW4114Escherichia coli (strain K12)BacteriaPseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichiaLocation and nucleotide sequence of frdB, the gene coding for the iron-sulphur protein subunit of the fumarate reductase of Escherichia coli.NUCLEOTIDE SEQUENCE [GENOMIC DNA]PROTEIN SEQUENCE OF 2-9Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The complete genome sequence of Escherichia coli K-12.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Overlap between ampC and frd operons on the Escherichia coli chromosome.NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 236-244Site-directed mutagenesis of conserved cysteine residues in Escherichia coli fumarate reductase: modification of the spectroscopic and electrochemical properties of the [2Fe-2S] cluster.MUTAGENESIS OF CYS-58; CYS-63; CYS-66 AND CYS-78Escherichia coli proteome analysis using the gene-protein database.IDENTIFICATION BY 2D-GELStructure of the Escherichia coli fumarate reductase respiratory complex.X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S); IRON-SULFUR (3FE-4S); IRON-SULFUR (4FE-4S) AND MENAQUINONECOFACTORSUBUNITSUBCELLULAR LOCATIONCrystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site.X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-244 IN COMPLEX WITH IRON-SULFUR (2FE-2S); IRON-SULFUR (3FE-4S); IRON-SULFUR (4FE-4S); MENAQUINONE AND INHIBITORSSUBUNITTwo distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used during anaerobic growth, and succinate dehydrogenase is used during aerobic growth. The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol-binding sites are functionally relevant (PubMed:10373108, PubMed:11850430).a quinone + succinate = a quinol + fumaratea menaquinone + succinate = a menaquinol + fumarate[2Fe-2S] clusterBinds 1 [2Fe-2S] cluster.[3Fe-4S] clusterBinds 1 [3Fe-4S] cluster.[4Fe-4S] clusterBinds 1 [4Fe-4S] cluster.Part of an enzyme complex containing four subunits: a flavoprotein (FrdA), an iron-sulfur protein (FrdB), and two hydrophobic anchor proteins (FrdC and FrdD).P0AC47P00363false4P0AC47P08337false4P0AC47P0A915false2Cell inner membranePeripheral membrane proteinCytoplasmic sideBelongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.2Fe-2S3D-structure3Fe-4S4Fe-4SCell inner membraneCell membraneDirect protein sequencingElectron transportIronIron-sulfurMembraneMetal-bindingOxidoreductaseReference proteomeTransportTricarboxylic acid cyclea menaquinone[2Fe-2S] cluster[2Fe-2S] cluster[2Fe-2S] cluster[2Fe-2S] cluster[4Fe-4S] cluster[4Fe-4S] cluster[4Fe-4S] cluster[3Fe-4S] cluster[3Fe-4S] cluster[3Fe-4S] cluster[4Fe-4S] clustera menaquinoneCSCSCSCSMAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR
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