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P0AC43

- SDHA_ECO57

UniProt

P0AC43 - SDHA_ECO57

Protein

Succinate dehydrogenase flavoprotein subunit

Gene

sdhA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.By similarity

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei221 – 2211FADBy similarity
    Binding sitei242 – 2421SubstrateBy similarity
    Binding sitei254 – 2541SubstrateBy similarity
    Active sitei286 – 2861Proton acceptorBy similarity
    Binding sitei354 – 3541SubstrateBy similarity
    Binding sitei388 – 3881FADBy similarity
    Binding sitei399 – 3991SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 196FADBy similarity
    Nucleotide bindingi37 – 5216FADBy similarityAdd
    BLAST
    Nucleotide bindingi404 – 4052FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. electron transport chain Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-743-MONOMER.
    ECOO157:SDHA-MONOMER.
    UniPathwayiUPA00223; UER01005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase flavoprotein subunit (EC:1.3.5.1)
    Gene namesi
    Name:sdhA
    Ordered Locus Names:Z0877, ECs0748
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cell inner membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 588588Succinate dehydrogenase flavoprotein subunitPRO_0000158653Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451Tele-8alpha-FAD histidineBy similarity
    Modified residuei267 – 2671N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP0AC43.

    Interactioni

    Subunit structurei

    Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein. The complex forms trimers By similarity.By similarity

    Protein-protein interaction databases

    STRINGi155864.Z0877.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC43.
    SMRiP0AC43. Positions 1-588.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1053.
    HOGENOMiHOG000160475.
    KOiK00239.
    OMAiRTEQGRI.
    OrthoDBiEOG6M3PC4.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AC43-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLPVREFDA VVIGAGGAGM RAALQISQSG QTCALLSKVF PTRSHTVSAQ    50
    GGITVALGNT HEDNWEWHMY DTVKGSDYIG DQDAIEYMCK TGPEAILELE 100
    HMGLPFSRLD DGRIYQRPFG GQSKNFGGEQ AARTAAAADR TGHALLHTLY 150
    QQNLKNHTTI FSEWYALDLV KNQDGAVVGC TALCIETGEV VYFKARATVL 200
    ATGGAGRIYQ STTNAHINTG DGVGMAIRAG VPVQDMEMWQ FHPTGIAGAG 250
    VLVTEGCRGE GGYLLNKHGE RFMERYAPNA KDLAGRDVVA RSIMIEIREG 300
    RGCDGPWGPH AKLKLDHLGK EVLESRLPGI LELSRTFAHV DPVKEPIPVI 350
    PTCHYMMGGI PTKVTGQALT VNEKGEDVVV PGLFAVGEIA CVSVHGANRL 400
    GGNSLLDLVV FGRAAGLHLQ ESIAEQGALR DASESDVEAS LDRLNRWNNN 450
    RNGEDPVAIR KALQECMQHN FSVFREGDAM AKGLEQLKVI RERLKNARLD 500
    DTSSEFNTQR VECLELDNLM ETAYATAVSA NFRTESRGAH SRFDFPDRDD 550
    ENWLCHSLYL PESESMTRRS VNMEPKLRPA FPPKIRTY 588
    Length:588
    Mass (Da):64,422
    Last modified:November 8, 2005 - v1
    Checksum:i837F9A63991B6CE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55047.1.
    BA000007 Genomic DNA. Translation: BAB34171.1.
    PIRiD90722.
    RefSeqiNP_286439.1. NC_002655.2.
    NP_308775.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG55047; AAG55047; Z0877.
    BAB34171; BAB34171; BAB34171.
    GeneIDi917480.
    957829.
    KEGGiece:Z0877.
    ecs:ECs0748.
    PATRICi18350518. VBIEscCol44059_0770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55047.1 .
    BA000007 Genomic DNA. Translation: BAB34171.1 .
    PIRi D90722.
    RefSeqi NP_286439.1. NC_002655.2.
    NP_308775.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0AC43.
    SMRi P0AC43. Positions 1-588.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z0877.

    Proteomic databases

    PRIDEi P0AC43.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG55047 ; AAG55047 ; Z0877 .
    BAB34171 ; BAB34171 ; BAB34171 .
    GeneIDi 917480.
    957829.
    KEGGi ece:Z0877.
    ecs:ECs0748.
    PATRICi 18350518. VBIEscCol44059_0770.

    Phylogenomic databases

    eggNOGi COG1053.
    HOGENOMi HOG000160475.
    KOi K00239.
    OMAi RTEQGRI.
    OrthoDBi EOG6M3PC4.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01005 .
    BioCyci ECOL386585:GJFA-743-MONOMER.
    ECOO157:SDHA-MONOMER.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiSDHA_ECO57
    AccessioniPrimary (citable) accession number: P0AC43
    Secondary accession number(s): P10444, P78282
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3