P0AC43 (DHSA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase flavoprotein subunit
EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	Z0877, ECs0748

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth By similarity.
Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	FAD.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein. The complex forms trimers By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Succinate dehydrogenase flavoprotein subunit		PRO_0000158653
Regions
	Nucleotide binding	14 – 19	6	FAD By similarity
	Nucleotide binding	37 – 52	16	FAD By similarity
	Nucleotide binding	404 – 405	2	FAD By similarity
Sites
	Active site	286	1	Proton acceptor By similarity
	Binding site	221	1	FAD By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Binding site	354	1	Substrate By similarity
	Binding site	388	1	FAD By similarity
	Binding site	399	1	Substrate By similarity
Amino acid modifications
	Modified residue	45	1	Tele-8alpha-FAD histidine By similarity
	Modified residue	267	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AC43 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 837F9A63991B6CE8	FASTA	588	64,422
	10 20 30 40 50 60 MKLPVREFDA VVIGAGGAGM RAALQISQSG QTCALLSKVF PTRSHTVSAQ GGITVALGNT 70 80 90 100 110 120 HEDNWEWHMY DTVKGSDYIG DQDAIEYMCK TGPEAILELE HMGLPFSRLD DGRIYQRPFG 130 140 150 160 170 180 GQSKNFGGEQ AARTAAAADR TGHALLHTLY QQNLKNHTTI FSEWYALDLV KNQDGAVVGC 190 200 210 220 230 240 TALCIETGEV VYFKARATVL ATGGAGRIYQ STTNAHINTG DGVGMAIRAG VPVQDMEMWQ 250 260 270 280 290 300 FHPTGIAGAG VLVTEGCRGE GGYLLNKHGE RFMERYAPNA KDLAGRDVVA RSIMIEIREG 310 320 330 340 350 360 RGCDGPWGPH AKLKLDHLGK EVLESRLPGI LELSRTFAHV DPVKEPIPVI PTCHYMMGGI 370 380 390 400 410 420 PTKVTGQALT VNEKGEDVVV PGLFAVGEIA CVSVHGANRL GGNSLLDLVV FGRAAGLHLQ 430 440 450 460 470 480 ESIAEQGALR DASESDVEAS LDRLNRWNNN RNGEDPVAIR KALQECMQHN FSVFREGDAM 490 500 510 520 530 540 AKGLEQLKVI RERLKNARLD DTSSEFNTQR VECLELDNLM ETAYATAVSA NFRTESRGAH 550 560 570 580 SRFDFPDRDD ENWLCHSLYL PESESMTRRS VNMEPKLRPA FPPKIRTY

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG55047.1. BA000007 Genomic DNA. Translation: BAB34171.1.
PIR	D90722.
RefSeq	NP_286439.1. NC_002655.2. NP_308775.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AC43.
SMR	P0AC43. Positions 1-588.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z0877.
Proteomic databases
PRIDE	P0AC43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG55047; AAG55047; Z0877. BAB34171; BAB34171; BAB34171.
GeneID	917480. 957829.
KEGG	ece:Z0877. ecs:ECs0748.
PATRIC	18350518. VBIEscCol44059_0770.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1053.
HOGENOM	HOG000160475.
KO	K00239.
OMA	VGCTAID.
ProtClustDB	PRK08958.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-743-MONOMER.
UniPathway	UPA00223; UER01005.
Family and domain databases
Gene3D	1.20.58.100. 1 hit.
InterPro	IPR003953. FAD_bind_dom. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_FrdA_Gneg. [Graphical view]
PANTHER	PTHR11632:SF5. PTHR11632:SF5. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_ECO57

Accession

Primary (citable) accession number: P0AC43
Secondary accession number(s): P10444, P78282

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program