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Protein

Succinate dehydrogenase flavoprotein subunit

Gene

sdhA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.3 Publications

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.1 Publication

Cofactori

FAD3 Publications

Enzyme regulationi

Inhibited by oxaloacetate.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (bacterial route).1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase flavoprotein subunit (sdhA), Succinate dehydrogenase iron-sulfur subunit (sdhB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (bacterial route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei221FAD3 Publications1
Binding sitei242Substrate1 Publication1
Binding sitei254Substrate1 Publication1
Active sitei286Proton acceptor1 Publication1
Binding sitei354Substrate1 Publication1
Binding sitei388FAD3 Publications1
Binding sitei399Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19FAD3 Publications6
Nucleotide bindingi37 – 52FAD3 PublicationsAdd BLAST16
Nucleotide bindingi404 – 405FAD3 Publications2

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • flavin adenine dinucleotide binding Source: EcoliWiki
  • succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  • succinate dehydrogenase activity Source: EcoliWiki

GO - Biological processi

  • aerobic respiration Source: EcoliWiki
  • anaerobic respiration Source: GO_Central
  • electron transport chain Source: InterPro
  • oxidation-reduction process Source: EcoliWiki
  • tricarboxylic acid cycle Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:SDH-FLAVO.
ECOL316407:JW0713-MONOMER.
MetaCyc:SDH-FLAVO.
BRENDAi1.3.5.1. 2026.
UniPathwayiUPA00223; UER01005.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase flavoprotein subunit (EC:1.3.5.11 Publication)
Gene namesi
Name:sdhA
Ordered Locus Names:b0723, JW0713
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10931. sdhA.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: EcoliWiki
  • plasma membrane succinate dehydrogenase complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001586521 – 588Succinate dehydrogenase flavoprotein subunitAdd BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45Tele-8alpha-FAD histidine3 Publications1
Modified residuei267N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0AC41.
PaxDbiP0AC41.
PRIDEiP0AC41.

2D gel databases

SWISS-2DPAGEP0AC41.

Interactioni

Subunit structurei

Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein. The complex forms trimers.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sdhBP070142EBI-371263,EBI-1035514

Protein-protein interaction databases

BioGridi4262907. 6 interactors.
DIPiDIP-31877N.
IntActiP0AC41. 91 interactors.
MINTiMINT-6478272.
STRINGi511145.b0723.

Structurei

Secondary structure

1588
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 13Combined sources9
Helixi17 – 28Combined sources12
Beta strandi33 – 39Combined sources7
Helixi41 – 43Combined sources3
Helixi45 – 48Combined sources4
Beta strandi59 – 61Combined sources3
Helixi65 – 75Combined sources11
Turni76 – 78Combined sources3
Helixi82 – 101Combined sources20
Beta strandi112 – 114Combined sources3
Beta strandi119 – 121Combined sources3
Turni125 – 127Combined sources3
Beta strandi129 – 135Combined sources7
Helixi141 – 155Combined sources15
Beta strandi159 – 162Combined sources4
Beta strandi164 – 171Combined sources8
Beta strandi177 – 184Combined sources8
Turni185 – 187Combined sources3
Beta strandi190 – 200Combined sources11
Helixi206 – 208Combined sources3
Beta strandi209 – 214Combined sources6
Helixi221 – 228Combined sources8
Beta strandi233 – 235Combined sources3
Beta strandi239 – 246Combined sources8
Turni247 – 249Combined sources3
Helixi256 – 259Combined sources4
Beta strandi263 – 265Combined sources3
Helixi273 – 276Combined sources4
Turni278 – 280Combined sources3
Helixi281 – 283Combined sources3
Helixi286 – 298Combined sources13
Beta strandi305 – 307Combined sources3
Beta strandi309 – 314Combined sources6
Helixi316 – 318Combined sources3
Helixi319 – 326Combined sources8
Helixi328 – 337Combined sources10
Turni342 – 344Combined sources3
Beta strandi347 – 356Combined sources10
Beta strandi359 – 362Combined sources4
Beta strandi367 – 371Combined sources5
Beta strandi373 – 375Combined sources3
Beta strandi377 – 385Combined sources9
Helixi387 – 389Combined sources3
Beta strandi393 – 395Combined sources3
Helixi403 – 417Combined sources15
Helixi419 – 426Combined sources8
Helixi434 – 439Combined sources6
Helixi442 – 449Combined sources8
Beta strandi452 – 454Combined sources3
Helixi456 – 470Combined sources15
Beta strandi471 – 475Combined sources5
Helixi477 – 494Combined sources18
Beta strandi504 – 506Combined sources3
Helixi508 – 532Combined sources25
Beta strandi542 – 545Combined sources4
Helixi550 – 553Combined sources4
Beta strandi554 – 560Combined sources7
Turni561 – 564Combined sources4
Beta strandi565 – 569Combined sources5
Beta strandi576 – 578Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60A1-588[»]
1NENX-ray2.90A1-588[»]
2ACZX-ray3.10A1-588[»]
2AD0model-A1-588[»]
2WDQX-ray2.40A/E/I1-588[»]
2WDRX-ray3.20A/E/I1-588[»]
2WDVX-ray3.20A/E/I1-588[»]
2WP9X-ray2.70A/E/I1-588[»]
2WS3X-ray3.20A/E/I1-588[»]
2WU2X-ray2.50A/E/I1-588[»]
2WU5X-ray2.80A/E/I1-588[»]
ProteinModelPortaliP0AC41.
SMRiP0AC41.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC41.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C00. Bacteria.
COG1053. LUCA.
HOGENOMiHOG000160475.
InParanoidiP0AC41.
KOiK00239.
OMAiCWEEAHR.
PhylomeDBiP0AC41.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLPVREFDA VVIGAGGAGM RAALQISQSG QTCALLSKVF PTRSHTVSAQ
60 70 80 90 100
GGITVALGNT HEDNWEWHMY DTVKGSDYIG DQDAIEYMCK TGPEAILELE
110 120 130 140 150
HMGLPFSRLD DGRIYQRPFG GQSKNFGGEQ AARTAAAADR TGHALLHTLY
160 170 180 190 200
QQNLKNHTTI FSEWYALDLV KNQDGAVVGC TALCIETGEV VYFKARATVL
210 220 230 240 250
ATGGAGRIYQ STTNAHINTG DGVGMAIRAG VPVQDMEMWQ FHPTGIAGAG
260 270 280 290 300
VLVTEGCRGE GGYLLNKHGE RFMERYAPNA KDLAGRDVVA RSIMIEIREG
310 320 330 340 350
RGCDGPWGPH AKLKLDHLGK EVLESRLPGI LELSRTFAHV DPVKEPIPVI
360 370 380 390 400
PTCHYMMGGI PTKVTGQALT VNEKGEDVVV PGLFAVGEIA CVSVHGANRL
410 420 430 440 450
GGNSLLDLVV FGRAAGLHLQ ESIAEQGALR DASESDVEAS LDRLNRWNNN
460 470 480 490 500
RNGEDPVAIR KALQECMQHN FSVFREGDAM AKGLEQLKVI RERLKNARLD
510 520 530 540 550
DTSSEFNTQR VECLELDNLM ETAYATAVSA NFRTESRGAH SRFDFPDRDD
560 570 580
ENWLCHSLYL PESESMTRRS VNMEPKLRPA FPPKIRTY
Length:588
Mass (Da):64,422
Last modified:November 8, 2005 - v1
Checksum:i837F9A63991B6CE8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20 – 22MRA → IAR in AAA23895 (PubMed:6383359).Curated3
Sequence conflicti20 – 22MRA → IAR in CAA25487 (PubMed:6383359).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01619 Genomic DNA. Translation: AAA23895.1.
X00980 Genomic DNA. Translation: CAA25487.1.
U00096 Genomic DNA. Translation: AAC73817.1.
AP009048 Genomic DNA. Translation: BAA35390.1.
X01070 Genomic DNA. Translation: CAA25533.1.
PIRiB64808. DEECSF.
RefSeqiNP_415251.1. NC_000913.3.
WP_000775540.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73817; AAC73817; b0723.
BAA35390; BAA35390; BAA35390.
GeneIDi945402.
KEGGiecj:JW0713.
eco:b0723.
PATRICi32116641. VBIEscCol129921_0753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01619 Genomic DNA. Translation: AAA23895.1.
X00980 Genomic DNA. Translation: CAA25487.1.
U00096 Genomic DNA. Translation: AAC73817.1.
AP009048 Genomic DNA. Translation: BAA35390.1.
X01070 Genomic DNA. Translation: CAA25533.1.
PIRiB64808. DEECSF.
RefSeqiNP_415251.1. NC_000913.3.
WP_000775540.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60A1-588[»]
1NENX-ray2.90A1-588[»]
2ACZX-ray3.10A1-588[»]
2AD0model-A1-588[»]
2WDQX-ray2.40A/E/I1-588[»]
2WDRX-ray3.20A/E/I1-588[»]
2WDVX-ray3.20A/E/I1-588[»]
2WP9X-ray2.70A/E/I1-588[»]
2WS3X-ray3.20A/E/I1-588[»]
2WU2X-ray2.50A/E/I1-588[»]
2WU5X-ray2.80A/E/I1-588[»]
ProteinModelPortaliP0AC41.
SMRiP0AC41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262907. 6 interactors.
DIPiDIP-31877N.
IntActiP0AC41. 91 interactors.
MINTiMINT-6478272.
STRINGi511145.b0723.

2D gel databases

SWISS-2DPAGEP0AC41.

Proteomic databases

EPDiP0AC41.
PaxDbiP0AC41.
PRIDEiP0AC41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73817; AAC73817; b0723.
BAA35390; BAA35390; BAA35390.
GeneIDi945402.
KEGGiecj:JW0713.
eco:b0723.
PATRICi32116641. VBIEscCol129921_0753.

Organism-specific databases

EchoBASEiEB0924.
EcoGeneiEG10931. sdhA.

Phylogenomic databases

eggNOGiENOG4105C00. Bacteria.
COG1053. LUCA.
HOGENOMiHOG000160475.
InParanoidiP0AC41.
KOiK00239.
OMAiCWEEAHR.
PhylomeDBiP0AC41.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01005.
BioCyciEcoCyc:SDH-FLAVO.
ECOL316407:JW0713-MONOMER.
MetaCyc:SDH-FLAVO.
BRENDAi1.3.5.1. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AC41.
PROiP0AC41.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHA_ECOLI
AccessioniPrimary (citable) accession number: P0AC41
Secondary accession number(s): P10444, P78282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.