SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0AC34

- FUMA_ECOL6

UniProt

P0AC34 - FUMA_ECOL6

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Fumarate hydratase class I, aerobic

Gene
fumA, c2004
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically By similarity.

Catalytic activityi

(S)-malate = fumarate + H2O.

Cofactori

Binds 1 4Fe-4S cluster By similarity.

Enzyme regulationi

Subject to aerobic respiratory control and catabolite repression.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi318 – 3181Iron-sulfur (4Fe-4S) By similarity
Active sitei397 – 3971 Reviewed prediction
Binding sitei463 – 4631Substrate Reviewed prediction

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. fumarate hydratase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciECOL199310:C2004-MONOMER.
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class I, aerobic (EC:4.2.1.2)
Short name:
Fumarase
Gene namesi
Name:fumA
Ordered Locus Names:c2004
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 548547Fumarate hydratase class I, aerobicPRO_0000195658Add
BLAST

Proteomic databases

PRIDEiP0AC34.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi199310.c2004.

Structurei

3D structure databases

ProteinModelPortaliP0AC34.
SMRiP0AC34. Positions 356-537.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I fumarase family.

Phylogenomic databases

HOGENOMiHOG000009338.
KOiK01676.
OMAiTCVEYPE.
OrthoDBiEOG6TXR10.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC34-1 [UniParc]FASTAAdd to Basket

« Hide

MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL    50
ARQAFHDASF MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG 100
VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA 150
PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCIAKGGGSA NKTYLYQETK 200
ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA 250
KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 300
IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL 350
RKAGEGEAVR VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL 400
KERMDNGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ 450
LQAQGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKSL 500
ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK 548
Length:548
Mass (Da):60,299
Last modified:January 23, 2007 - v2
Checksum:i6082744088C64603
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80464.1.
RefSeqiNP_753899.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN80464; AAN80464; c2004.
GeneIDi1036044.
KEGGiecc:c2004.
PATRICi18281905. VBIEscCol75197_1871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80464.1 .
RefSeqi NP_753899.1. NC_004431.1.

3D structure databases

ProteinModelPortali P0AC34.
SMRi P0AC34. Positions 356-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 199310.c2004.

Proteomic databases

PRIDEi P0AC34.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN80464 ; AAN80464 ; c2004 .
GeneIDi 1036044.
KEGGi ecc:c2004.
PATRICi 18281905. VBIEscCol75197_1871.

Phylogenomic databases

HOGENOMi HOG000009338.
KOi K01676.
OMAi TCVEYPE.
OrthoDBi EOG6TXR10.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .
BioCyci ECOL199310:C2004-MONOMER.

Family and domain databases

Gene3Di 3.20.130.10. 1 hit.
InterProi IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view ]
Pfami PF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMi SSF117457. SSF117457. 1 hit.
TIGRFAMsi TIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiFUMA_ECOL6
AccessioniPrimary (citable) accession number: P0AC34
Secondary accession number(s): P00923, P76889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi