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P0AC34

- FUMA_ECOL6

UniProt

P0AC34 - FUMA_ECOL6

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Protein

Fumarate hydratase class I, aerobic

Gene

fumA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible hydration of fumarate to (S)-malate. Functions as an aerobic enzyme in the direction of malate formation as part of the citric acid cycle. Accounts for about 80% of the fumarase activity when the bacteria grow aerobically. To a lesser extent, also displays D-tartrate dehydratase activity in vitro, but is not able to convert (R)-malate, L-tartrate or meso-tartrate. Can also catalyze the isomerization of enol- to keto-oxaloacetate.By similarity

Catalytic activityi

(S)-malate = fumarate + H2O.By similarity
Keto-oxaloacetate = enol-oxaloacetate.By similarity

Cofactori

Binds 1 4Fe-4S cluster per subunit.By similarity

Pathwayi

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. fumarate hydratase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciECOL199310:C2004-MONOMER.
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class I, aerobicBy similarity (EC:4.2.1.2By similarity)
Alternative name(s):
Fumarase ABy similarity
Oxaloacetate keto--enol-isomeraseBy similarity
Short name:
OAAKE isomeraseBy similarity
Oxaloacetate tautomeraseBy similarity (EC:5.3.2.2By similarity)
Gene namesi
Name:fumABy similarity
Ordered Locus Names:c2004
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 548547Fumarate hydratase class I, aerobicPRO_0000195658Add
BLAST

Proteomic databases

PRIDEiP0AC34.

Expressioni

Inductioni

Is expressed under aerobic conditions. Is repressed by glucose and anaerobiosis.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi199310.c2004.

Structurei

3D structure databases

ProteinModelPortaliP0AC34.
SMRiP0AC34. Positions 356-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I fumarase family.Curated

Phylogenomic databases

HOGENOMiHOG000009338.
KOiK01676.
OMAiTCVEYPE.
OrthoDBiEOG6TXR10.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC34-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL
60 70 80 90 100
ARQAFHDASF MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG
110 120 130 140 150
VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA
160 170 180 190 200
PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCIAKGGGSA NKTYLYQETK
210 220 230 240 250
ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA
260 270 280 290 300
KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV
310 320 330 340 350
IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL
360 370 380 390 400
RKAGEGEAVR VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL
410 420 430 440 450
KERMDNGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ
460 470 480 490 500
LQAQGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKSL
510 520 530 540
ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK
Length:548
Mass (Da):60,299
Last modified:January 23, 2007 - v2
Checksum:i6082744088C64603
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80464.1.
RefSeqiNP_753899.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN80464; AAN80464; c2004.
GeneIDi1036044.
KEGGiecc:c2004.
PATRICi18281905. VBIEscCol75197_1871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80464.1 .
RefSeqi NP_753899.1. NC_004431.1.

3D structure databases

ProteinModelPortali P0AC34.
SMRi P0AC34. Positions 356-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 199310.c2004.

Proteomic databases

PRIDEi P0AC34.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN80464 ; AAN80464 ; c2004 .
GeneIDi 1036044.
KEGGi ecc:c2004.
PATRICi 18281905. VBIEscCol75197_1871.

Phylogenomic databases

HOGENOMi HOG000009338.
KOi K01676.
OMAi TCVEYPE.
OrthoDBi EOG6TXR10.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .
BioCyci ECOL199310:C2004-MONOMER.

Family and domain databases

Gene3Di 3.20.130.10. 1 hit.
InterProi IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view ]
Pfami PF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMi SSF117457. SSF117457. 1 hit.
TIGRFAMsi TIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiFUMA_ECOL6
AccessioniPrimary (citable) accession number: P0AC34
Secondary accession number(s): P00923, P76889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3