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P0AC34

- FUMA_ECOL6

UniProt

P0AC34 - FUMA_ECOL6

Protein

Fumarate hydratase class I, aerobic

Gene

fumA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically By similarity.By similarity

    Catalytic activityi

    (S)-malate = fumarate + H2O.

    Cofactori

    Binds 1 4Fe-4S cluster.By similarity

    Enzyme regulationi

    Subject to aerobic respiratory control and catabolite repression.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi318 – 3181Iron-sulfur (4Fe-4S)By similarity
    Active sitei397 – 3971Sequence Analysis
    Binding sitei463 – 4631SubstrateSequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. fumarate hydratase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL199310:C2004-MONOMER.
    UniPathwayiUPA00223; UER01007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fumarate hydratase class I, aerobic (EC:4.2.1.2)
    Short name:
    Fumarase
    Gene namesi
    Name:fumA
    Ordered Locus Names:c2004
    OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
    Taxonomic identifieri199310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001410: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 548547Fumarate hydratase class I, aerobicPRO_0000195658Add
    BLAST

    Proteomic databases

    PRIDEiP0AC34.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi199310.c2004.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC34.
    SMRiP0AC34. Positions 356-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-I fumarase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000009338.
    KOiK01676.
    OMAiTCVEYPE.
    OrthoDBiEOG6TXR10.

    Family and domain databases

    Gene3Di3.20.130.10. 1 hit.
    InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view]
    PfamiPF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMiSSF117457. SSF117457. 1 hit.
    TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AC34-1 [UniParc]FASTAAdd to Basket

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    MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL    50
    ARQAFHDASF MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG 100
    VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA 150
    PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCIAKGGGSA NKTYLYQETK 200
    ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA 250
    KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 300
    IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL 350
    RKAGEGEAVR VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL 400
    KERMDNGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ 450
    LQAQGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKSL 500
    ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK 548
    Length:548
    Mass (Da):60,299
    Last modified:January 23, 2007 - v2
    Checksum:i6082744088C64603
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN80464.1.
    RefSeqiNP_753899.1. NC_004431.1.

    Genome annotation databases

    EnsemblBacteriaiAAN80464; AAN80464; c2004.
    GeneIDi1036044.
    KEGGiecc:c2004.
    PATRICi18281905. VBIEscCol75197_1871.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN80464.1 .
    RefSeqi NP_753899.1. NC_004431.1.

    3D structure databases

    ProteinModelPortali P0AC34.
    SMRi P0AC34. Positions 356-537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 199310.c2004.

    Proteomic databases

    PRIDEi P0AC34.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN80464 ; AAN80464 ; c2004 .
    GeneIDi 1036044.
    KEGGi ecc:c2004.
    PATRICi 18281905. VBIEscCol75197_1871.

    Phylogenomic databases

    HOGENOMi HOG000009338.
    KOi K01676.
    OMAi TCVEYPE.
    OrthoDBi EOG6TXR10.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01007 .
    BioCyci ECOL199310:C2004-MONOMER.

    Family and domain databases

    Gene3Di 3.20.130.10. 1 hit.
    InterProi IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view ]
    Pfami PF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMi SSF117457. SSF117457. 1 hit.
    TIGRFAMsi TIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFT073 / ATCC 700928 / UPEC.

    Entry informationi

    Entry nameiFUMA_ECOL6
    AccessioniPrimary (citable) accession number: P0AC34
    Secondary accession number(s): P00923, P76889
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3