Reviewed, UniProtKB/Swiss-Prot P0AC33 (FUMA_ECOLI)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class I, aerobic
      Short name=Fumarase
    EC=4.2.1.2

Gene names

Name:	fumA
Ordered Locus Names:	b1612, JW1604

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically.
Catalytic activity	(S)-malate = fumarate + H2O.
Cofactor	Binds 1 4Fe-4S cluster.
Enzyme regulation	Subject to aerobic respiratory control and catabolite repression.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the class-I fumarase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW fumarate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 548	547	Fumarate hydratase class I, aerobic		PRO_0000195656
Sites
	Active site	397	1	Potential
	Metal binding	318	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	463	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AC33-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6082744088C64603	FASTA	548	60,299
	10 20 30 40 50 60 MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL ARQAFHDASF 70 80 90 100 110 120 MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG VLPTCQDTGT AIIVGKKGQR 130 140 150 160 170 180 VWTGGGDEAA LARGVYNTYI EDNLRYSQNA PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF 190 200 210 220 230 240 LCIAKGGGSA NKTYLYQETK ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET 250 260 270 280 290 300 NLKTVKLASA KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 310 320 330 340 350 360 IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL RKAGEGEAVR 370 380 390 400 410 420 VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL KERMDNGEGL PQYIKDHPIY 430 440 450 460 470 480 YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ LQAQGGSMIM LAKGNRSQQV TDACKKHGGF 490 500 510 520 530 540 YLGSIGGPAA VLAQGSIKSL ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli." Miles J.S., Guest J.R. Nucleic Acids Res. 12:3631-3642(1984) [PubMed: 6328431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Purification and characterization of two types of fumarase from Escherichia coli." Ueda Y., Yumoto N., Tokushige M., Fukui K., Ohya-Nishiguchi H. J. Biochem. 109:728-733(1991) [PubMed: 1917897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21. Strain: W / ATCC 11105 / DSM 1900.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / EMG2.
[7]	"The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC)." Guest J.R., Miles J.S., Roberts R.E., Woods S.A. J. Gen. Microbiol. 131:2971-2984(1985) [PubMed: 3005475] [Abstract] Cited for: IDENTIFICATION OF THE STRUCTURAL GENE.
[8]	"Two biochemically distinct classes of fumarase in Escherichia coli." Woods S.A., Shwartzbach S.D., Guest J.R. Biochim. Biophys. Acta 954:14-26(1988) [PubMed: 3282546] [Abstract] Cited for: BIOCHEMICAL ANALYSIS OF FUMA AND FUMC.
[9]	"Fumarase A from E. coli contains a [4Fe-4S] cluster." Flint D.H., Emptage M.H., Guest J.R. J. Inorg. Biochem. 36:306-306(1989) Cited for: IRON-SULFUR CLUSTER.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X00522 Genomic DNA. Translation: CAA25204.1. AP009048 Genomic DNA. Translation: BAA15360.1. U00096 Genomic DNA. Translation: AAC74684.1.
PIR	UFECAQ. A03531.
RefSeq	AP_002233.1. NP_416129.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AC33. 5 interactions.
STRING	P0AC33.
Genome annotation databases
GeneID	946826.
GenomeReviews	Gene locus JW1604 in contig AP009048_GR. Gene locus b1612 in contig U00096_GR.
KEGG	ecj:JW1604. eco:b1612.
Organism-specific databases
EchoBASE	EB0351.
EcoGene	EG10356. fumA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AC33.
OMA	FQQIQTS.
Enzyme and pathway databases
BioCyc	EcoCyc:FUMA-MON. MetaCyc:FUMA-MON.
Gene expression databases
Genevestigator	P0AC33.
Family and domain databases
InterPro	IPR011167. Fe_dep_fumarate_hydratase. IPR020557. Fumarate_lyase_CS. IPR004646. TtdA_fumA_fumB. IPR004647. TtdB_fumA_fumB. [Graphical view]
Gene3D	G3DSA:3.20.130.10. TtdB_fumA_fumB. 1 hit.
Pfam	PF05681. Fumerase. 1 hit. PF05683. Fumerase_C. 1 hit. [Graphical view]
PIRSF	PIRSF001394. Fe_dep_fumar_hy. 1 hit.
TIGRFAMs	TIGR00722. ttdA_fumA_fumB. 1 hit. TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMA_ECOLI

Accession

Primary (citable) accession number: P0AC33
Secondary accession number(s): P00923, P76889

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)