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P0AC33 (FUMA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase class I, aerobic

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fumA
Ordered Locus Names:b1612, JW1604
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically.

Catalytic activity

(S)-malate = fumarate + H2O.

Cofactor

Binds 1 4Fe-4S cluster.

Enzyme regulation

Subject to aerobic respiratory control and catabolite repression.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the class-I fumarase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-549075,EBI-549075

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 548547Fumarate hydratase class I, aerobic
PRO_0000195656

Sites

Active site3971 Potential
Metal binding3181Iron-sulfur (4Fe-4S) By similarity
Binding site4631Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P0AC33 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 6082744088C64603

FASTA54860,299
        10         20         30         40         50         60 
MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL ARQAFHDASF 

        70         80         90        100        110        120 
MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG VLPTCQDTGT AIIVGKKGQR 

       130        140        150        160        170        180 
VWTGGGDEAA LARGVYNTYI EDNLRYSQNA PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF 

       190        200        210        220        230        240 
LCIAKGGGSA NKTYLYQETK ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET 

       250        260        270        280        290        300 
NLKTVKLASA KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 

       310        320        330        340        350        360 
IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL RKAGEGEAVR 

       370        380        390        400        410        420 
VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL KERMDNGEGL PQYIKDHPIY 

       430        440        450        460        470        480 
YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ LQAQGGSMIM LAKGNRSQQV TDACKKHGGF 

       490        500        510        520        530        540 
YLGSIGGPAA VLAQGSIKSL ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL 


TQCTRCVK 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli."
Miles J.S., Guest J.R.
Nucleic Acids Res. 12:3631-3642(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Purification and characterization of two types of fumarase from Escherichia coli."
Ueda Y., Yumoto N., Tokushige M., Fukui K., Ohya-Nishiguchi H.
J. Biochem. 109:728-733(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
Strain: W / ATCC 11105 / DSM 1900.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-12.
Strain: K12 / EMG2.
[7]"The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC)."
Guest J.R., Miles J.S., Roberts R.E., Woods S.A.
J. Gen. Microbiol. 131:2971-2984(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF THE STRUCTURAL GENE.
[8]"Two biochemically distinct classes of fumarase in Escherichia coli."
Woods S.A., Shwartzbach S.D., Guest J.R.
Biochim. Biophys. Acta 954:14-26(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOCHEMICAL ANALYSIS OF FUMA AND FUMC.
[9]"Fumarase A from E. coli contains a [4Fe-4S] cluster."
Flint D.H., Emptage M.H., Guest J.R.
J. Inorg. Biochem. 36:306-306(1989)
Cited for: IRON-SULFUR CLUSTER.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00522 Genomic DNA. Translation: CAA25204.1.
AP009048 Genomic DNA. Translation: BAA15360.1.
U00096 Genomic DNA. Translation: AAC74684.1.
PIRUFECAQ. A03531.
RefSeqNP_416129.1. NC_000913.3.
YP_489875.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AC33.
SMRP0AC33. Positions 356-537.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-36200N.
IntActP0AC33. 5 interactions.
MINTMINT-1310290.
STRING511145.b1612.

Proteomic databases

PaxDbP0AC33.
PRIDEP0AC33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74684; AAC74684; b1612.
BAA15360; BAA15360; BAA15360.
GeneID12934128.
946826.
KEGGecj:Y75_p1588.
eco:b1612.
PATRIC32118526. VBIEscCol129921_1683.

Organism-specific databases

EchoBASEEB0351.
EcoGeneEG10356. fumA.

Phylogenomic databases

eggNOGCOG1838.
HOGENOMHOG000009338.
KOK01676.
OMATCVEYPE.
OrthoDBEOG6TXR10.
PhylomeDBP0AC33.

Enzyme and pathway databases

BioCycEcoCyc:FUMA-MONOMER.
ECOL316407:JW1604-MONOMER.
MetaCyc:FUMA-MONOMER.
RETL1328306-WGS:GSTH-2399-MONOMER.
SABIO-RKP0AC33.
UniPathwayUPA00223; UER01007.

Gene expression databases

GenevestigatorP0AC33.

Family and domain databases

Gene3D3.20.130.10. 1 hit.
InterProIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMSSF117457. SSF117457. 1 hit.
TIGRFAMsTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AC33.

Entry information

Entry nameFUMA_ECOLI
AccessionPrimary (citable) accession number: P0AC33
Secondary accession number(s): P00923, P76889
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene