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P0AC33

- FUMA_ECOLI

UniProt

P0AC33 - FUMA_ECOLI

Protein

Fumarate hydratase class I, aerobic

Gene

fumA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically.

    Catalytic activityi

    (S)-malate = fumarate + H2O.

    Cofactori

    Binds 1 4Fe-4S cluster.

    Enzyme regulationi

    Subject to aerobic respiratory control and catabolite repression.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi318 – 3181Iron-sulfur (4Fe-4S)By similarity
    Active sitei397 – 3971Sequence Analysis
    Binding sitei463 – 4631SubstrateSequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: EcoCyc
    2. fumarate hydratase activity Source: EcoCyc
    3. identical protein binding Source: IntAct
    4. metal ion binding Source: UniProtKB-KW
    5. oxaloacetate tautomerase activity Source: EcoCyc

    GO - Biological processi

    1. tricarboxylic acid cycle Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:FUMA-MONOMER.
    ECOL316407:JW1604-MONOMER.
    MetaCyc:FUMA-MONOMER.
    RETL1328306-WGS:GSTH-2399-MONOMER.
    SABIO-RKP0AC33.
    UniPathwayiUPA00223; UER01007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fumarate hydratase class I, aerobic (EC:4.2.1.2)
    Short name:
    Fumarase
    Gene namesi
    Name:fumA
    Ordered Locus Names:b1612, JW1604
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10356. fumA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 548547Fumarate hydratase class I, aerobicPRO_0000195656Add
    BLAST

    Proteomic databases

    PaxDbiP0AC33.
    PRIDEiP0AC33.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AC33.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-549075,EBI-549075

    Protein-protein interaction databases

    DIPiDIP-36200N.
    IntActiP0AC33. 5 interactions.
    MINTiMINT-1310290.
    STRINGi511145.b1612.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC33.
    SMRiP0AC33. Positions 356-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-I fumarase family.Curated

    Phylogenomic databases

    eggNOGiCOG1838.
    HOGENOMiHOG000009338.
    KOiK01676.
    OMAiTCVEYPE.
    OrthoDBiEOG6TXR10.
    PhylomeDBiP0AC33.

    Family and domain databases

    Gene3Di3.20.130.10. 1 hit.
    InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view]
    PfamiPF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMiSSF117457. SSF117457. 1 hit.
    TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AC33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS VSEFEGQEIL KVAPEALTLL    50
    ARQAFHDASF MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG 100
    VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA 150
    PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCIAKGGGSA NKTYLYQETK 200
    ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA 250
    KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 300
    IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL 350
    RKAGEGEAVR VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL 400
    KERMDNGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ 450
    LQAQGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKSL 500
    ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK 548
    Length:548
    Mass (Da):60,299
    Last modified:January 23, 2007 - v2
    Checksum:i6082744088C64603
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00522 Genomic DNA. Translation: CAA25204.1.
    AP009048 Genomic DNA. Translation: BAA15360.1.
    U00096 Genomic DNA. Translation: AAC74684.1.
    PIRiA03531. UFECAQ.
    RefSeqiNP_416129.1. NC_000913.3.
    YP_489875.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74684; AAC74684; b1612.
    BAA15360; BAA15360; BAA15360.
    GeneIDi12934128.
    946826.
    KEGGiecj:Y75_p1588.
    eco:b1612.
    PATRICi32118526. VBIEscCol129921_1683.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00522 Genomic DNA. Translation: CAA25204.1 .
    AP009048 Genomic DNA. Translation: BAA15360.1 .
    U00096 Genomic DNA. Translation: AAC74684.1 .
    PIRi A03531. UFECAQ.
    RefSeqi NP_416129.1. NC_000913.3.
    YP_489875.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AC33.
    SMRi P0AC33. Positions 356-537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36200N.
    IntActi P0AC33. 5 interactions.
    MINTi MINT-1310290.
    STRINGi 511145.b1612.

    Proteomic databases

    PaxDbi P0AC33.
    PRIDEi P0AC33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74684 ; AAC74684 ; b1612 .
    BAA15360 ; BAA15360 ; BAA15360 .
    GeneIDi 12934128.
    946826.
    KEGGi ecj:Y75_p1588.
    eco:b1612.
    PATRICi 32118526. VBIEscCol129921_1683.

    Organism-specific databases

    EchoBASEi EB0351.
    EcoGenei EG10356. fumA.

    Phylogenomic databases

    eggNOGi COG1838.
    HOGENOMi HOG000009338.
    KOi K01676.
    OMAi TCVEYPE.
    OrthoDBi EOG6TXR10.
    PhylomeDBi P0AC33.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01007 .
    BioCyci EcoCyc:FUMA-MONOMER.
    ECOL316407:JW1604-MONOMER.
    MetaCyc:FUMA-MONOMER.
    RETL1328306-WGS:GSTH-2399-MONOMER.
    SABIO-RK P0AC33.

    Miscellaneous databases

    PROi P0AC33.

    Gene expression databases

    Genevestigatori P0AC33.

    Family and domain databases

    Gene3Di 3.20.130.10. 1 hit.
    InterProi IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view ]
    Pfami PF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMi SSF117457. SSF117457. 1 hit.
    TIGRFAMsi TIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli."
      Miles J.S., Guest J.R.
      Nucleic Acids Res. 12:3631-3642(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Purification and characterization of two types of fumarase from Escherichia coli."
      Ueda Y., Yumoto N., Tokushige M., Fukui K., Ohya-Nishiguchi H.
      J. Biochem. 109:728-733(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
      Strain: W / ATCC 11105 / DSM 1900.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-12.
      Strain: K12 / EMG2.
    7. "The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC)."
      Guest J.R., Miles J.S., Roberts R.E., Woods S.A.
      J. Gen. Microbiol. 131:2971-2984(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF THE STRUCTURAL GENE.
    8. "Two biochemically distinct classes of fumarase in Escherichia coli."
      Woods S.A., Shwartzbach S.D., Guest J.R.
      Biochim. Biophys. Acta 954:14-26(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOCHEMICAL ANALYSIS OF FUMA AND FUMC.
    9. "Fumarase A from E. coli contains a [4Fe-4S] cluster."
      Flint D.H., Emptage M.H., Guest J.R.
      J. Inorg. Biochem. 36:306-306(1989)
      Cited for: IRON-SULFUR CLUSTER.

    Entry informationi

    Entry nameiFUMA_ECOLI
    AccessioniPrimary (citable) accession number: P0AC33
    Secondary accession number(s): P00923, P76889
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3