Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell division protein FtsX

Gene

ftsX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter FtsEX involved in cellular division. Important for assembly or stability of the septal ring. Encoded in an operon consisting of genes ftsY, ftsE and ftsX.2 Publications

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:FTSX-MONOMER.
ECOL316407:JW3427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein FtsX
Gene namesi
Name:ftsX
Synonyms:ftsS
Ordered Locus Names:b3462, JW3427
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10345. ftsX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicSequence analysisAdd
BLAST
Transmembranei75 – 9521HelicalSequence analysisAdd
BLAST
Topological domaini96 – 223128PeriplasmicSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 27531CytoplasmicSequence analysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Topological domaini297 – 32226PeriplasmicSequence analysisAdd
BLAST
Transmembranei323 – 34321HelicalSequence analysisAdd
BLAST
Topological domaini344 – 3529CytoplasmicSequence analysis

GO - Cellular componenti

  • cell division site Source: EcoliWiki
  • Gram-negative-bacterium-type cell wall Source: UniProtKB
  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Double deletion mutant FtsEX grows well on plates with LB medium with 1% NaCl forming healthy colonies at 30 degrees Celsius, whereas at 37 or 42 degrees Celsius, the colonies are very tiny and sick. In culture medium it exhibits mild elongation of cells at 30 degrees Celsius, and become very extensive at higher temperatures. This double mutant does not grow on LB culture medium without NaCl (LBON) at any temperature, possibly due to excessive lethal filamentation. Supplementation with any of a variety of osmolytes, such as glucose, sucrose, glycerol, or NaCl, completely rescues the growth on LBON medium. Though the growth is completely inhibited on LBON plates, the cultures grown in LBON broth show an initial increase in cell mass before the cessation of growth, and at this stage, the culture is extremely filamentous. The filaments grown in LB at 42 degrees Celsius or in LBON at 30 degrees Celsius are very long and appear to have elongated cells. They are normal and regularly spaced without any significant chromosomal aberrations or partition defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Cell division protein FtsXPRO_0000166792Add
BLAST

Proteomic databases

PaxDbiP0AC30.
PRIDEiP0AC30.

Interactioni

Subunit structurei

Forms a membrane-associated complex with FtsE.

Protein-protein interaction databases

BioGridi4262498. 546 interactions.
DIPiDIP-35993N.
IntActiP0AC30. 5 interactions.
STRINGi511145.b3462.

Structurei

3D structure databases

ProteinModelPortaliP0AC30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CRP. Bacteria.
COG2177. LUCA.
HOGENOMiHOG000270397.
InParanoidiP0AC30.
KOiK09811.
OMAiITIFARR.
OrthoDBiEOG60W7WW.
PhylomeDBiP0AC30.

Family and domain databases

InterProiIPR003838. ABC_permease_dom.
IPR004513. ABC_transpt_FtsX.
[Graphical view]
PfamiPF02687. FtsX. 1 hit.
[Graphical view]
PIRSFiPIRSF003097. FtsX. 1 hit.
TIGRFAMsiTIGR00439. ftsX. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRDAINHI RQFGGRLDRF RKSVGGSGDG GRNAPKRAKS SPKPVNRKTN
60 70 80 90 100
VFNEQVRYAF HGALQDLKSK PFATFLTVMV IAISLTLPSV CYMVYKNVNQ
110 120 130 140 150
AATQYYPSPQ ITVYLQKTLD DDAAAGVVAQ LQAEQGVEKV NYLSREDALG
160 170 180 190 200
EFRNWSGFGG ALDMLEENPL PAVAVVIPKL DFQGTESLNT LRDRITQING
210 220 230 240 250
IDEVRMDDSW FARLAALTGL VGRVSAMIGV LMVAAVFLVI GNSVRLSIFA
260 270 280 290 300
RRDSINVQKL IGATDGFILR PFLYGGALLG FSGALLSLIL SEILVLRLSS
310 320 330 340 350
AVAEVAQVFG TKFDINGLSF DECLLLLLVC SMIGWVAAWL ATVQHLRHFT

PE
Length:352
Mass (Da):38,544
Last modified:November 8, 2005 - v1
Checksum:iC006ED56043739AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04398 Genomic DNA. Translation: CAA27986.1.
U00039 Genomic DNA. Translation: AAB18437.1.
U00096 Genomic DNA. Translation: AAC76487.1.
AP009048 Genomic DNA. Translation: BAE77831.1.
PIRiS03132. CEECFX.
RefSeqiNP_417919.1. NC_000913.3.
WP_001042003.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76487; AAC76487; b3462.
BAE77831; BAE77831; BAE77831.
GeneIDi947969.
KEGGiecj:JW3427.
eco:b3462.
PATRICi32122368. VBIEscCol129921_3561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04398 Genomic DNA. Translation: CAA27986.1.
U00039 Genomic DNA. Translation: AAB18437.1.
U00096 Genomic DNA. Translation: AAC76487.1.
AP009048 Genomic DNA. Translation: BAE77831.1.
PIRiS03132. CEECFX.
RefSeqiNP_417919.1. NC_000913.3.
WP_001042003.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262498. 546 interactions.
DIPiDIP-35993N.
IntActiP0AC30. 5 interactions.
STRINGi511145.b3462.

Proteomic databases

PaxDbiP0AC30.
PRIDEiP0AC30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76487; AAC76487; b3462.
BAE77831; BAE77831; BAE77831.
GeneIDi947969.
KEGGiecj:JW3427.
eco:b3462.
PATRICi32122368. VBIEscCol129921_3561.

Organism-specific databases

EchoBASEiEB0341.
EcoGeneiEG10345. ftsX.

Phylogenomic databases

eggNOGiENOG4105CRP. Bacteria.
COG2177. LUCA.
HOGENOMiHOG000270397.
InParanoidiP0AC30.
KOiK09811.
OMAiITIFARR.
OrthoDBiEOG60W7WW.
PhylomeDBiP0AC30.

Enzyme and pathway databases

BioCyciEcoCyc:FTSX-MONOMER.
ECOL316407:JW3427-MONOMER.

Miscellaneous databases

PROiP0AC30.

Family and domain databases

InterProiIPR003838. ABC_permease_dom.
IPR004513. ABC_transpt_FtsX.
[Graphical view]
PfamiPF02687. FtsX. 1 hit.
[Graphical view]
PIRSFiPIRSF003097. FtsX. 1 hit.
TIGRFAMsiTIGR00439. ftsX. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new cell division operon in Escherichia coli."
    Gill D.R., Hatfull G.F., Salmond G.P.C.
    Mol. Gen. Genet. 205:134-145(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Genetic analysis of essential genes in the ftsE region of the Escherichia coli genetic map and identification of a new cell division gene, ftsS."
    Salmond G.P., Plakidou S.
    Mol. Gen. Genet. 197:304-308(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  6. "The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated."
    Gill D.R., Salmond G.P.
    Mol. Gen. Genet. 210:504-508(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, OPERON STRUCTURE.
  7. Cited for: FUNCTION, INTERACTION WITH FTSE, SUBCELLULAR LOCATION, OVEREXPRESSION.
  8. "A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli."
    Schmidt K.L., Peterson N.D., Kustusch R.J., Wissel M.C., Graham B., Phillips G.J., Weiss D.S.
    J. Bacteriol. 186:785-793(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  10. "Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength."
    Reddy M.
    J. Bacteriol. 189:98-108(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiFTSX_ECOLI
AccessioniPrimary (citable) accession number: P0AC30
Secondary accession number(s): P10122, Q2M7C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 13, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression leads to strong inhibition of growth, cell filamentation and eventually cell death.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.