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Protein

Cell division protein FtsX

Gene

ftsX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter FtsEX involved in cellular division. Important for assembly or stability of the septal ring. Encoded in an operon consisting of genes ftsY, ftsE and ftsX.2 Publications

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:FTSX-MONOMER.
ECOL316407:JW3427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein FtsX
Gene namesi
Name:ftsX
Synonyms:ftsS
Ordered Locus Names:b3462, JW3427
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10345. ftsX.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 223PeriplasmicSequence analysisAdd BLAST128
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Topological domaini245 – 275CytoplasmicSequence analysisAdd BLAST31
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 322PeriplasmicSequence analysisAdd BLAST26
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Topological domaini344 – 352CytoplasmicSequence analysis9

GO - Cellular componenti

  • cell division site Source: EcoliWiki
  • Gram-negative-bacterium-type cell wall Source: UniProtKB
  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Double deletion mutant FtsEX grows well on plates with LB medium with 1% NaCl forming healthy colonies at 30 degrees Celsius, whereas at 37 or 42 degrees Celsius, the colonies are very tiny and sick. In culture medium it exhibits mild elongation of cells at 30 degrees Celsius, and become very extensive at higher temperatures. This double mutant does not grow on LB culture medium without NaCl (LBON) at any temperature, possibly due to excessive lethal filamentation. Supplementation with any of a variety of osmolytes, such as glucose, sucrose, glycerol, or NaCl, completely rescues the growth on LBON medium. Though the growth is completely inhibited on LBON plates, the cultures grown in LBON broth show an initial increase in cell mass before the cessation of growth, and at this stage, the culture is extremely filamentous. The filaments grown in LB at 42 degrees Celsius or in LBON at 30 degrees Celsius are very long and appear to have elongated cells. They are normal and regularly spaced without any significant chromosomal aberrations or partition defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001667921 – 352Cell division protein FtsXAdd BLAST352

Proteomic databases

PaxDbiP0AC30.
PRIDEiP0AC30.

Interactioni

Subunit structurei

Forms a membrane-associated complex with FtsE.

Protein-protein interaction databases

BioGridi4262498. 546 interactors.
DIPiDIP-35993N.
IntActiP0AC30. 5 interactors.
STRINGi511145.b3462.

Structurei

3D structure databases

ProteinModelPortaliP0AC30.
SMRiP0AC30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CRP. Bacteria.
COG2177. LUCA.
HOGENOMiHOG000270397.
InParanoidiP0AC30.
KOiK09811.
OMAiITIFARR.
PhylomeDBiP0AC30.

Family and domain databases

InterProiIPR003838. ABC_permease_dom.
IPR004513. ABC_transpt_FtsX.
[Graphical view]
PfamiPF02687. FtsX. 1 hit.
[Graphical view]
PIRSFiPIRSF003097. FtsX. 1 hit.
TIGRFAMsiTIGR00439. ftsX. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRDAINHI RQFGGRLDRF RKSVGGSGDG GRNAPKRAKS SPKPVNRKTN
60 70 80 90 100
VFNEQVRYAF HGALQDLKSK PFATFLTVMV IAISLTLPSV CYMVYKNVNQ
110 120 130 140 150
AATQYYPSPQ ITVYLQKTLD DDAAAGVVAQ LQAEQGVEKV NYLSREDALG
160 170 180 190 200
EFRNWSGFGG ALDMLEENPL PAVAVVIPKL DFQGTESLNT LRDRITQING
210 220 230 240 250
IDEVRMDDSW FARLAALTGL VGRVSAMIGV LMVAAVFLVI GNSVRLSIFA
260 270 280 290 300
RRDSINVQKL IGATDGFILR PFLYGGALLG FSGALLSLIL SEILVLRLSS
310 320 330 340 350
AVAEVAQVFG TKFDINGLSF DECLLLLLVC SMIGWVAAWL ATVQHLRHFT

PE
Length:352
Mass (Da):38,544
Last modified:November 8, 2005 - v1
Checksum:iC006ED56043739AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04398 Genomic DNA. Translation: CAA27986.1.
U00039 Genomic DNA. Translation: AAB18437.1.
U00096 Genomic DNA. Translation: AAC76487.1.
AP009048 Genomic DNA. Translation: BAE77831.1.
PIRiS03132. CEECFX.
RefSeqiNP_417919.1. NC_000913.3.
WP_001042003.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76487; AAC76487; b3462.
BAE77831; BAE77831; BAE77831.
GeneIDi947969.
KEGGiecj:JW3427.
eco:b3462.
PATRICi32122368. VBIEscCol129921_3561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04398 Genomic DNA. Translation: CAA27986.1.
U00039 Genomic DNA. Translation: AAB18437.1.
U00096 Genomic DNA. Translation: AAC76487.1.
AP009048 Genomic DNA. Translation: BAE77831.1.
PIRiS03132. CEECFX.
RefSeqiNP_417919.1. NC_000913.3.
WP_001042003.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC30.
SMRiP0AC30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262498. 546 interactors.
DIPiDIP-35993N.
IntActiP0AC30. 5 interactors.
STRINGi511145.b3462.

Proteomic databases

PaxDbiP0AC30.
PRIDEiP0AC30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76487; AAC76487; b3462.
BAE77831; BAE77831; BAE77831.
GeneIDi947969.
KEGGiecj:JW3427.
eco:b3462.
PATRICi32122368. VBIEscCol129921_3561.

Organism-specific databases

EchoBASEiEB0341.
EcoGeneiEG10345. ftsX.

Phylogenomic databases

eggNOGiENOG4105CRP. Bacteria.
COG2177. LUCA.
HOGENOMiHOG000270397.
InParanoidiP0AC30.
KOiK09811.
OMAiITIFARR.
PhylomeDBiP0AC30.

Enzyme and pathway databases

BioCyciEcoCyc:FTSX-MONOMER.
ECOL316407:JW3427-MONOMER.

Miscellaneous databases

PROiP0AC30.

Family and domain databases

InterProiIPR003838. ABC_permease_dom.
IPR004513. ABC_transpt_FtsX.
[Graphical view]
PfamiPF02687. FtsX. 1 hit.
[Graphical view]
PIRSFiPIRSF003097. FtsX. 1 hit.
TIGRFAMsiTIGR00439. ftsX. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFTSX_ECOLI
AccessioniPrimary (citable) accession number: P0AC30
Secondary accession number(s): P10122, Q2M7C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression leads to strong inhibition of growth, cell filamentation and eventually cell death.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.