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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

ygfA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the removal of 5-formyltetrahydrofolate. In vitro, it is a potent inhibitor of various folate-dependent enzymes in the C1 metabolism network and in vivo it might function as a folate storage. 5-formyltetrahydrofolate is also used as an antifolate rescue agent in cancer chemotherapy. Catalyzes the irreversible ATP-dependent transformation of 5-formyltetrahydrofolate (5-CHO-THF) to form 5,10-methenyltetrahydrofolate (5,10-CH=THF). The reverse reaction is catalyzed by the serine hydroxymethyltransferase GlyA (SHMT) (By similarity).By similarity

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei167ATPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 135ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Short name:
5-FCL
Alternative name(s):
5,10-methenyltetrahydrofolate synthetase
Short name:
MTHFS
Gene namesi
Name:ygfA
Ordered Locus Names:Z4249, ECs3782
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002002851 – 1825-formyltetrahydrofolate cyclo-ligaseAdd BLAST182

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4249

Structurei

3D structure databases

ProteinModelPortaliP0AC29
SMRiP0AC29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105MK2 Bacteria
COG0212 LUCA
HOGENOMiHOG000007300
KOiK01934
OMAiPERPSEM

Family and domain databases

Gene3Di3.40.50.10420, 1 hit
InterProiView protein in InterPro
IPR002698 FTHF_cligase
IPR024185 FTHF_cligase-like_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23407:SF1 PTHR23407:SF1, 1 hit
PfamiView protein in Pfam
PF01812 5-FTHF_cyc-lig, 1 hit
PIRSFiPIRSF006806 FTHF_cligase, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit
TIGRFAMsiTIGR02727 MTHFS_bact, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQRRRALT PEQQQEMGQQ AATRMMTYPP VVMAHTVAVF LSFDGELDTQ
60 70 80 90 100
PLIEQLWRAG KRVYLPVLHP FSAGNLLFLN YHPQSELVMN RLKIHEPKLD
110 120 130 140 150
VRDVLPLSRL DVLITPLVAF DEYGQRLGMG GGFYDRTLQN WQHYKTQPVG
160 170 180
YAHDCQLVEK LPVEEWDIPL PAVVTPSKVW EW
Length:182
Mass (Da):21,105
Last modified:November 8, 2005 - v1
Checksum:i389B13098552BF81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58038.1
BA000007 Genomic DNA Translation: BAB37205.1
PIRiF91101
RefSeqiNP_311809.3, NC_002695.1

Genome annotation databases

EnsemblBacteriaiAAG58038; AAG58038; Z4249
BAB37205; BAB37205; BAB37205
GeneIDi916397
KEGGiece:Z4249
ecs:ECs3782
PATRICifig|386585.9.peg.3947

Entry informationi

Entry namei5FCL_ECO57
AccessioniPrimary (citable) accession number: P0AC29
Secondary accession number(s): P09160
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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