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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

ygfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the removal of 5-formyltetrahydrofolate. In vitro, it is a potent inhibitor of various folate-dependent enzymes in the C1 metabolism network and in vivo it might function as a folate storage. 5-formyltetrahydrofolate is also used as an antifolate rescue agent in cancer chemotherapy. Catalyzes the irreversible ATP-dependent transformation of 5-formyltetrahydrofolate (5-CHO-THF) to form 5,10-methenyltetrahydrofolate (5,10-CH=THF). The reverse reaction is catalyzed by the serine hydroxymethyltransferase GlyA (SHMT).2 Publications

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei167ATPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 135ATPSequence analysis8

GO - Molecular functioni

  • 5-formyltetrahydrofolate cyclo-ligase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • dormancy process Source: EcoCyc
  • folic acid-containing compound biosynthetic process Source: GO_Central
  • tetrahydrofolate interconversion Source: GO_Central

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11158-MONOMER
MetaCyc:EG11158-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Short name:
5-FCL
Alternative name(s):
5,10-methenyltetrahydrofolate synthetase
Short name:
MTHFS
Gene namesi
Name:ygfA
Ordered Locus Names:b2912, JW2879
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11158 ygfA

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene does not cause a growth defect in rich or minimal medium. However, when grown in minimal medium with added glycine, the mutant accumulates 5-CHO-THF. If the only nitrogen source in minimal medium is glycine, the mutant shows a severe growth defect, likely due to inhibition of serine hydroxymethyltransferase (SHMT) and the glycine cleavage system by the accumulated 5-CHO-THF. This phenotype can be complemented by the glutamate formiminotransferase (FT).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002002841 – 1825-formyltetrahydrofolate cyclo-ligaseAdd BLAST182

Proteomic databases

PaxDbiP0AC28
PRIDEiP0AC28

Expressioni

Inductioni

During biofilm formation.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ndkP0A7635EBI-555094,EBI-370139

Protein-protein interaction databases

BioGridi4261171, 227 interactors
DIPiDIP-48194N
IntActiP0AC28, 9 interactors
STRINGi316385.ECDH10B_3086

Structurei

3D structure databases

ProteinModelPortaliP0AC28
SMRiP0AC28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105MK2 Bacteria
COG0212 LUCA
HOGENOMiHOG000007300
InParanoidiP0AC28
KOiK01934
OMAiPERPSEM
PhylomeDBiP0AC28

Family and domain databases

Gene3Di3.40.50.10420, 1 hit
InterProiView protein in InterPro
IPR002698 FTHF_cligase
IPR024185 FTHF_cligase-like_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23407:SF1 PTHR23407:SF1, 1 hit
PfamiView protein in Pfam
PF01812 5-FTHF_cyc-lig, 1 hit
PIRSFiPIRSF006806 FTHF_cligase, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit
TIGRFAMsiTIGR02727 MTHFS_bact, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQRRRALT PEQQQEMGQQ AATRMMTYPP VVMAHTVAVF LSFDGELDTQ
60 70 80 90 100
PLIEQLWRAG KRVYLPVLHP FSAGNLLFLN YHPQSELVMN RLKIHEPKLD
110 120 130 140 150
VRDVLPLSRL DVLITPLVAF DEYGQRLGMG GGFYDRTLQN WQHYKTQPVG
160 170 180
YAHDCQLVEK LPVEEWDIPL PAVVTPSKVW EW
Length:182
Mass (Da):21,105
Last modified:November 8, 2005 - v1
Checksum:i389B13098552BF81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12965 Genomic DNA Translation: AAA24651.1
U28377 Genomic DNA Translation: AAA69079.1
U00096 Genomic DNA Translation: AAC75949.1
AP009048 Genomic DNA Translation: BAE76976.1
PIRiA21894 QQEC2K
RefSeqiNP_417387.3, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC75949; AAC75949; b2912
BAE76976; BAE76976; BAE76976
GeneIDi945167
KEGGiecj:JW2879
eco:b2912
PATRICifig|511145.12.peg.3006

Entry informationi

Entry namei5FCL_ECOLI
AccessioniPrimary (citable) accession number: P0AC28
Secondary accession number(s): P09160, Q2M9T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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