P0AC28 (YGFA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein ygfA

Gene names

Name:	ygfA
Ordered Locus Names:	b2912, JW2879

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dormancy process Inferred from mutant phenotype. Source: EcoCyc folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-formyltetrahydrofolate cyclo-ligase activity Inferred from mutant phenotype. Source: EcoCyc ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ndk	P0A763	3	EBI-555094,EBI-370139

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Uncharacterized protein ygfA		PRO_0000200284
Regions
	Nucleotide binding	128 – 135	8	ATP Potential
Sites
	Binding site	167	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AC28 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 389B13098552BF81	FASTA	182	21,105
	10 20 30 40 50 60 MIRQRRRALT PEQQQEMGQQ AATRMMTYPP VVMAHTVAVF LSFDGELDTQ PLIEQLWRAG 70 80 90 100 110 120 KRVYLPVLHP FSAGNLLFLN YHPQSELVMN RLKIHEPKLD VRDVLPLSRL DVLITPLVAF 130 140 150 160 170 180 DEYGQRLGMG GGFYDRTLQN WQHYKTQPVG YAHDCQLVEK LPVEEWDIPL PAVVTPSKVW EW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli 6S RNA gene is part of a dual-function transcription unit." Hsu L.M., Zagorski J., Wang Z., Fournier M.J. J. Bacteriol. 161:1162-1170(1985) [PubMed: 2579060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12965 Genomic DNA. Translation: AAA24651.1. U28377 Genomic DNA. Translation: AAA69079.1. U00096 Genomic DNA. Translation: AAC75949.1. AP009048 Genomic DNA. Translation: BAE76976.1.
PIR	QQEC2K. A21894.
RefSeq	NP_417387.3. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AC28.
SMR	P0AC28. Positions 2-182.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48194N.
IntAct	P0AC28. 9 interactions.
MINT	MINT-1249443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004125; EBESCP00000004125; EBESCG00000003367. EBESCT00000018206; EBESCP00000017497; EBESCG00000017261.
GeneID	945167.
GenomeReviews	Gene locus JW2879 in contig AP009048_GR. Gene locus b2912 in contig U00096_GR.
KEGG	ecj:JW2879. eco:b2912.
PATRIC	32121236. VBIEscCol129921_3006.
Organism-specific databases
EchoBASE	EB1147.
EcoGene	EG11158. ygfA.
Phylogenomic databases
eggNOG	COG0212.
GeneTree	EBGT00050000010573.
HOGENOM	HBG325345.
OMA	EQQRRFG.
PhylomeDB	P0AC28.
ProtClustDB	PRK10333.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11158-MONOMER. MetaCyc:EG11158-MONOMER.
Gene expression databases
Genevestigator	P0AC28.
Family and domain databases
InterPro	IPR002698. FTHF_cligase. IPR024185. FTHF_cligase-like. [Graphical view]
Gene3D	G3DSA:3.40.50.10420. FTHF_cligase. 1 hit.
Pfam	PF01812. 5-FTHF_cyc-lig. 1 hit. [Graphical view]
PIRSF	PIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMs	TIGR02727. MTHFS_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YGFA_ECOLI

Accession

Primary (citable) accession number: P0AC28
Secondary accession number(s): P09160, Q2M9T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program