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Protein

Nitrite transporter NirC

Gene

nirC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes nitrite uptake and nitrite export across the cytoplasmic membrane. Is up to 10-fold more active than NarK or NarU in nitrite uptake for subsequent reduction in the cytoplasm by the NirB/NirD nitrite reductase.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrate assimilation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NIRC-MONOMER.
ECOL316407:JW3330-MONOMER.
MetaCyc:NIRC-MONOMER.

Protein family/group databases

TCDBi1.A.16.3.1. the formate-nitrite transporter (fnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite transporter NirC
Gene namesi
Name:nirC
Ordered Locus Names:b3367, JW3330
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10654. nirC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4621HelicalSequence analysisAdd
BLAST
Topological domaini47 – 5913PeriplasmicSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Topological domaini81 – 11232CytoplasmicSequence analysisAdd
BLAST
Transmembranei113 – 13321HelicalSequence analysisAdd
BLAST
Topological domaini134 – 15118PeriplasmicSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 1797CytoplasmicSequence analysis
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Topological domaini201 – 22525PeriplasmicSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 26822CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Nitrite transporter NirCPRO_0000094724Add
BLAST

Proteomic databases

PaxDbiP0AC26.

Interactioni

Protein-protein interaction databases

BioGridi4262477. 13 interactions.
STRINGi511145.b3367.

Structurei

3D structure databases

ProteinModelPortaliP0AC26.
SMRiP0AC26. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107V9H. Bacteria.
COG2116. LUCA.
HOGENOMiHOG000271392.
InParanoidiP0AC26.
KOiK02598.
OMAiFLMKYAH.
OrthoDBiEOG661HBX.
PhylomeDBiP0AC26.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000292. For/NO2_transpt.
IPR024002. For/NO2_transpt_CS.
[Graphical view]
PfamiPF01226. Form_Nir_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00790. fnt. 1 hit.
PROSITEiPS01005. FORMATE_NITRITE_TP_1. 1 hit.
PS01006. FORMATE_NITRITE_TP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTDTINKCA ANAARIARLS ANNPLGFWVS SAMAGAYVGL GIILIFTLGN
60 70 80 90 100
LLDPSVRPLV MGATFGIALT LVIIAGSELF TGHTMFLTFG VKAGSISHGQ
110 120 130 140 150
MWAILPQTWL GNLVGSVFVA MLYSWGGGSL LPVDTSIVHS VALAKTTAPA
160 170 180 190 200
MVLFFKGALC NWLVCLAIWM ALRTEGAAKF IAIWWCLLAF IASGYEHSIA
210 220 230 240 250
NMTLFALSWF GNHSEAYTLA GIGHNLLWVT LGNTLSGAVF MGLGYWYATP
260
KANRPVADKF NQTETAAG
Length:268
Mass (Da):28,563
Last modified:November 8, 2005 - v1
Checksum:i6E01788EA8FB8F31
GO

Sequence cautioni

The sequence AAA58164.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA32418.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA. Translation: CAA32418.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58164.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76392.2.
AP009048 Genomic DNA. Translation: BAE77923.1.
PIRiB65131.
RefSeqiWP_000493556.1. NZ_CP014272.1.
YP_026212.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76392; AAC76392; b3367.
BAE77923; BAE77923; BAE77923.
GeneIDi2847757.
KEGGiecj:JW3330.
eco:b3367.
PATRICi32122168. VBIEscCol129921_3461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA. Translation: CAA32418.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58164.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76392.2.
AP009048 Genomic DNA. Translation: BAE77923.1.
PIRiB65131.
RefSeqiWP_000493556.1. NZ_CP014272.1.
YP_026212.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AC26.
SMRiP0AC26. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262477. 13 interactions.
STRINGi511145.b3367.

Protein family/group databases

TCDBi1.A.16.3.1. the formate-nitrite transporter (fnt) family.

Proteomic databases

PaxDbiP0AC26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76392; AAC76392; b3367.
BAE77923; BAE77923; BAE77923.
GeneIDi2847757.
KEGGiecj:JW3330.
eco:b3367.
PATRICi32122168. VBIEscCol129921_3461.

Organism-specific databases

EchoBASEiEB0648.
EcoGeneiEG10654. nirC.

Phylogenomic databases

eggNOGiENOG4107V9H. Bacteria.
COG2116. LUCA.
HOGENOMiHOG000271392.
InParanoidiP0AC26.
KOiK02598.
OMAiFLMKYAH.
OrthoDBiEOG661HBX.
PhylomeDBiP0AC26.

Enzyme and pathway databases

BioCyciEcoCyc:NIRC-MONOMER.
ECOL316407:JW3330-MONOMER.
MetaCyc:NIRC-MONOMER.

Miscellaneous databases

PROiP0AC26.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000292. For/NO2_transpt.
IPR024002. For/NO2_transpt_CS.
[Graphical view]
PfamiPF01226. Form_Nir_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00790. fnt. 1 hit.
PROSITEiPS01005. FORMATE_NITRITE_TP_1. 1 hit.
PS01006. FORMATE_NITRITE_TP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP."
    Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.
    Nucleic Acids Res. 17:3865-3874(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome."
    Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., Harborne N., Wootton J., Nicolson R., Cole J.A.
    Eur. J. Biochem. 191:315-323(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The roles of the polytopic membrane proteins NarK, NarU and NirC in Escherichia coli K-12: two nitrate and three nitrite transporters."
    Clegg S., Yu F., Griffiths L., Cole J.A.
    Mol. Microbiol. 44:143-155(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  6. "Nitrate and nitrite transport in Escherichia coli."
    Jia W., Cole J.A.
    Biochem. Soc. Trans. 33:159-161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "A single channel for nitrate uptake, nitrite export and nitrite uptake by Escherichia coli NarU and a role for NirC in nitrite export and uptake."
    Jia W., Tovell N., Clegg S., Trimmer M., Cole J.
    Biochem. J. 417:297-304(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNIRC_ECOLI
AccessioniPrimary (citable) accession number: P0AC26
Secondary accession number(s): P11097, P78112, Q2M733
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.