Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydroneopterin triphosphate 2'-epimerase

Gene

folX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of carbon 2' of the side chain of 7,8-dihydroneopterin triphosphate (H2NTP) to form 7,8-dihydromonapterin triphosphate (H2MTP) (PubMed:9182560) (PubMed:9651328). Is required for tetrahydromonapterin biosynthesis, a major pterin in E.coli (PubMed:19897652).3 Publications

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 7,8-dihydromonapterin 3'-triphosphate.2 Publications

Kineticsi

  1. KM=13 µM for 7,8-dihydroneopterin triphosphate1 Publication
  2. KM=149 µM for 7,8-dihydroneopterin1 Publication
  3. KM=66 µM for 7,8-dihydromonapterin1 Publication
  1. Vmax=480 µmol/h/mg enzyme for the epimerization of 7,8-dihydroneopterin triphosphate1 Publication
  2. Vmax=0.95 µmol/h/mg enzyme for the epimerization of 7,8-dihydroneopterin1 Publication
  3. Vmax=0.67 µmol/h/mg enzyme for the epimerization of 7,8-dihydromonapterin1 Publication

GO - Molecular functioni

GO - Biological processi

  • folic acid-containing compound metabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciEcoCyc:H2NTPEPIM-MONOMER.
ECOL316407:JW2300-MONOMER.
MetaCyc:H2NTPEPIM-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroneopterin triphosphate 2'-epimerase1 Publication (EC:5.1.99.-2 Publications)
Alternative name(s):
D-erythro-7,8-dihydroneopterin triphosphate epimerase
Gene namesi
Name:folX
Ordered Locus Names:b2303, JW2300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14263. folX.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show no detectable growth defect on complete and minimal medium (PubMed:9651328). The folX deletion selectively eliminates monapterin production and secretion, but has no effect on the intra- and extracellular folate profiles (PubMed:19897652).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001682952 – 120Dihydroneopterin triphosphate 2'-epimeraseAdd BLAST119

Proteomic databases

EPDiP0AC19.
PaxDbiP0AC19.
PRIDEiP0AC19.

Interactioni

Subunit structurei

Homooctamer.2 Publications

Protein-protein interaction databases

BioGridi4260518. 7 interactors.
DIPiDIP-9679N.
IntActiP0AC19. 3 interactors.
STRINGi511145.b2303.

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 19Combined sources15
Helixi23 – 27Combined sources5
Beta strandi30 – 41Combined sources12
Helixi42 – 46Combined sources5
Helixi47 – 49Combined sources3
Helixi57 – 69Combined sources13
Beta strandi71 – 74Combined sources4
Helixi76 – 87Combined sources12
Beta strandi94 – 103Combined sources10
Beta strandi109 – 119Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B9LX-ray2.90A/B/C/D/E/F/G/H1-120[»]
ProteinModelPortaliP0AC19.
SMRiP0AC19.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC19.

Family & Domainsi

Sequence similaritiesi

Belongs to the DHNA family.Curated

Phylogenomic databases

eggNOGiENOG4105KKP. Bacteria.
COG1539. LUCA.
HOGENOMiHOG000217626.
InParanoidiP0AC19.
KOiK07589.
OMAiTKAIIAH.
PhylomeDBiP0AC19.

Family and domain databases

CDDicd00534. DHNA_DHNTPE. 1 hit.
InterProiIPR006157. FolB_dom.
[Graphical view]
PfamiPF02152. FolB. 1 hit.
[Graphical view]
SMARTiSM00905. FolB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00526. folB_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPAAIIRI KNLRLRTFIG IKEEEINNRQ DIVINVTIHY PADKARTSED
60 70 80 90 100
INDALNYRTV TKNIIQHVEN NRFSLLEKLT QDVLDIAREH HWVTYAEVEI
110 120
DKLHALRYAD SVSMTLSWQR
Length:120
Mass (Da):14,082
Last modified:January 23, 2007 - v2
Checksum:i5DDFF76540827ED6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 30NNRQ → KQPS in AAB47972 (PubMed:9182560).Curated4
Sequence conflicti48 – 49SE → RQ in AAB47972 (PubMed:9182560).Curated2
Sequence conflicti55L → M in AAB47972 (PubMed:9182560).Curated1
Sequence conflicti107R → A in AAB47972 (PubMed:9182560).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96709 Genomic DNA. Translation: CAA65471.1.
U47639 Genomic DNA. Translation: AAB47972.1.
AP009048 Genomic DNA. Translation: BAA16140.1.
U00096 Genomic DNA. Translation: AAC75363.1.
PIRiE65002.
RefSeqiNP_416806.1. NC_000913.3.
WP_000068457.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75363; AAC75363; b2303.
BAA16140; BAA16140; BAA16140.
GeneIDi946781.
KEGGiecj:JW2300.
eco:b2303.
PATRICi32119977. VBIEscCol129921_2398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96709 Genomic DNA. Translation: CAA65471.1.
U47639 Genomic DNA. Translation: AAB47972.1.
AP009048 Genomic DNA. Translation: BAA16140.1.
U00096 Genomic DNA. Translation: AAC75363.1.
PIRiE65002.
RefSeqiNP_416806.1. NC_000913.3.
WP_000068457.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B9LX-ray2.90A/B/C/D/E/F/G/H1-120[»]
ProteinModelPortaliP0AC19.
SMRiP0AC19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260518. 7 interactors.
DIPiDIP-9679N.
IntActiP0AC19. 3 interactors.
STRINGi511145.b2303.

Proteomic databases

EPDiP0AC19.
PaxDbiP0AC19.
PRIDEiP0AC19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75363; AAC75363; b2303.
BAA16140; BAA16140; BAA16140.
GeneIDi946781.
KEGGiecj:JW2300.
eco:b2303.
PATRICi32119977. VBIEscCol129921_2398.

Organism-specific databases

EchoBASEiEB4011.
EcoGeneiEG14263. folX.

Phylogenomic databases

eggNOGiENOG4105KKP. Bacteria.
COG1539. LUCA.
HOGENOMiHOG000217626.
InParanoidiP0AC19.
KOiK07589.
OMAiTKAIIAH.
PhylomeDBiP0AC19.

Enzyme and pathway databases

BioCyciEcoCyc:H2NTPEPIM-MONOMER.
ECOL316407:JW2300-MONOMER.
MetaCyc:H2NTPEPIM-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AC19.
PROiP0AC19.

Family and domain databases

CDDicd00534. DHNA_DHNTPE. 1 hit.
InterProiIPR006157. FolB_dom.
[Graphical view]
PfamiPF02152. FolB. 1 hit.
[Graphical view]
SMARTiSM00905. FolB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00526. folB_dom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFOLX_ECOLI
AccessioniPrimary (citable) accession number: P0AC19
Secondary accession number(s): P77796, P80449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.