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P0AC11

- DHP2_ECOLX

UniProt

P0AC11 - DHP2_ECOLX

Protein

Dihydropteroate synthase type-2

Gene

sulII

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.

    Catalytic activityi

    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121MagnesiumBy similarity
    Binding sitei20 – 201SubstrateBy similarity
    Binding sitei85 – 851SubstrateBy similarity
    Binding sitei104 – 1041SubstrateBy similarity
    Binding sitei174 – 1741SubstrateBy similarity
    Binding sitei213 – 2131SubstrateBy similarity
    Binding sitei247 – 2471SubstrateBy similarity
    Binding sitei249 – 2491SubstrateBy similarity

    GO - Molecular functioni

    1. dihydropteroate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. response to antibiotic Source: UniProtKB-KW
    3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance, Folate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropteroate synthase type-2 (EC:2.5.1.15)
    Alternative name(s):
    Dihydropteroate pyrophosphorylase type II
    Dihydropteroate synthase type II
    Short name:
    DHPS
    Gene namesi
    Name:sulII
    Encoded oniPlasmid pGS051 Publication
    Plasmid pGS03B1 Publication
    Plasmid IncQ RSF10101 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Dihydropteroate synthase type-2PRO_0000168203Add
    BLAST

    Proteomic databases

    PRIDEiP0AC11.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC11.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 259259Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 532Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    InterProiIPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AC11-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKSLIIFGI VNITSDSFSD GGRYLAPDAA IAQARKLMAE GADVIDLGPA    50
    SSNPDAAPVS SDTEIARIAP VLDALKADGI PVSLDSYQPA TQAYALSRGV 100
    AYLNDIRGFP DAAFYPQLAK SSAKLVVMHS VQDGQADRRE APAGDIMDHI 150
    AAFFDARIAA LTGAGIKRNR LVLDPGMGFF LGAAPETSLS VLARFDELRL 200
    RFDLPVLLSV SRKSFLRALT GRGPGDVGAA TLAAELAAAA GGADFIRTHE 250
    PRPLRDGLAV LAALKETARI R 271
    Length:271
    Mass (Da):28,470
    Last modified:November 8, 2005 - v1
    Checksum:i4B00E71A3F7970FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36657 Genomic DNA. Translation: AAA24936.1.
    PIRiA34950.
    RefSeqiNP_957531.1. NC_005324.1.
    YP_002455756.1. NC_011795.1.
    YP_002891091.1. NC_012690.1.
    YP_002894395.1. NC_012692.1.
    YP_002995721.1. NC_012886.1.
    YP_006940094.1. NC_018995.1.
    YP_006952281.1. NC_019060.1.
    YP_006952747.1. NC_019065.1.
    YP_006952891.1. NC_019066.1.
    YP_006953258.1. NC_019070.1.
    YP_006953602.1. NC_019080.1.
    YP_007349479.1. NC_020086.1.
    YP_008574999.1. NC_022377.1.
    YP_794145.1. NC_008490.1.
    YP_891140.1. NC_008597.1.

    Genome annotation databases

    GeneIDi13877054.
    13905222.
    13905650.
    13905795.
    13906179.
    13906535.
    15152799.
    17035774.
    2716515.
    4397525.
    4525479.
    7205328.
    7872496.
    7872620.
    8094934.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36657 Genomic DNA. Translation: AAA24936.1 .
    PIRi A34950.
    RefSeqi NP_957531.1. NC_005324.1.
    YP_002455756.1. NC_011795.1.
    YP_002891091.1. NC_012690.1.
    YP_002894395.1. NC_012692.1.
    YP_002995721.1. NC_012886.1.
    YP_006940094.1. NC_018995.1.
    YP_006952281.1. NC_019060.1.
    YP_006952747.1. NC_019065.1.
    YP_006952891.1. NC_019066.1.
    YP_006953258.1. NC_019070.1.
    YP_006953602.1. NC_019080.1.
    YP_007349479.1. NC_020086.1.
    YP_008574999.1. NC_022377.1.
    YP_794145.1. NC_008490.1.
    YP_891140.1. NC_008597.1.

    3D structure databases

    ProteinModelPortali P0AC11.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0AC11.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13877054.
    13905222.
    13905650.
    13905795.
    13906179.
    13906535.
    15152799.
    17035774.
    2716515.
    4397525.
    4525479.
    7205328.
    7872496.
    7872620.
    8094934.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00156 .

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    InterProi IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 1 hit.
    TIGRFAMsi TIGR01496. DHPS. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase."
      Raedstroem P., Swedberg G.
      Antimicrob. Agents Chemother. 32:1684-1692(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncQ RSF1010 pGS03B pGS05

    Entry informationi

    Entry nameiDHP2_ECOLX
    AccessioniPrimary (citable) accession number: P0AC11
    Secondary accession number(s): P19539
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sulII gene is located on various IncQ (Broad-host-range) plasmids and other small non-conjugative resistance plasmids.

    Keywords - Technical termi

    Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3