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Protein

Dihydropteroate synthase type-2

Gene

sulII

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives (By similarity). It is implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.By similarity

Miscellaneous

The sulII gene is located on various IncQ (Broad-host-range) plasmids and other small non-conjugative resistance plasmids.

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase (folP), Dihydropteroate synthase (sul1), Dihydropteroate synthase (sul3), Dihydropteroate synthase, Dihydropteroate synthase (folP), Dihydropteroate synthase (CWS33_26340), Dihydropteroate synthase (folP), Dihydropteroate synthase, Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (AM333_01790), Dihydropteroate synthase (folP), Dihydropteroate synthase (SulI), Dihydropteroate synthase (BK383_15895), Dihydropteroate synthase (CRT55_03920), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (AW059_15240), Dihydropteroate synthase (BSR05_04210), Dihydropteroate synthase (folP), Dihydropteroate synthase type-1 (sulI), Dihydropteroate synthase (CRM83_20785), Dihydropteroate synthase, Dihydropteroate synthase (folP), Dihydropteroate synthase (sul2), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (C2U48_30365), Dihydropteroate synthase (BK375_28220), Dihydropteroate synthase (BMT49_25665), Dihydropteroate synthase (sul1), Dihydropteroate synthase (sul2), Dihydropteroate synthase (SM09_03452), Dihydropteroate synthase (folP_3), Dihydropteroate synthase (folP), Dihydropteroate synthase (APU18_05315), Dihydropteroate synthase (C1I39_27460), Dihydropteroate synthase (sul3), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul3), Dihydropteroate synthase (A9819_18240), Dihydropteroate synthase (sulI), Dihydropteroate synthase (ACN81_18515), Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul1), Dihydropteroate synthase (BK400_32640), Dihydropteroate synthase (C2U48_30480), Dihydropteroate synthase type-2 (sulII), Dihydropteroate synthase (B634_00023), Dihydropteroate synthase (CR534_03110), Dihydropteroate synthase (BXT93_15530), Dihydropteroate synthase (BMR14_26270), Dihydropteroate synthase (sul1), Dihydropteroate synthase (sulII), Dihydropteroate synthase, Dihydropteroate synthase (BMR22_19505), Dihydropteroate synthase (sulII), Dihydropteroate synthase (sul2)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12MagnesiumBy similarity1
Binding sitei856-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1046-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1746-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2136-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic resistance, Folate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-2 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type II
Dihydropteroate synthase type II
Short name:
DHPS
Gene namesi
Name:sulII
Encoded oniPlasmid pGS051 Publication
Plasmid pGS03B1 Publication
Plasmid IncQ RSF10101 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682031 – 271Dihydropteroate synthase type-2Add BLAST271

Interactioni

Subunit structurei

Homodimer.Curated

Structurei

3D structure databases

ProteinModelPortaliP0AC11
SMRiP0AC11
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 259Pterin-bindingPROSITE-ProRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 2496-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the DHPS family.Curated

Family and domain databases

CDDicd00739 DHPS, 1 hit
Gene3Di3.20.20.20, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF00809 Pterin_bind, 1 hit
SUPFAMiSSF51717 SSF51717, 1 hit
TIGRFAMsiTIGR01496 DHPS, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKSLIIFGI VNITSDSFSD GGRYLAPDAA IAQARKLMAE GADVIDLGPA
60 70 80 90 100
SSNPDAAPVS SDTEIARIAP VLDALKADGI PVSLDSYQPA TQAYALSRGV
110 120 130 140 150
AYLNDIRGFP DAAFYPQLAK SSAKLVVMHS VQDGQADRRE APAGDIMDHI
160 170 180 190 200
AAFFDARIAA LTGAGIKRNR LVLDPGMGFF LGAAPETSLS VLARFDELRL
210 220 230 240 250
RFDLPVLLSV SRKSFLRALT GRGPGDVGAA TLAAELAAAA GGADFIRTHE
260 270
PRPLRDGLAV LAALKETARI R
Length:271
Mass (Da):28,470
Last modified:November 8, 2005 - v1
Checksum:i4B00E71A3F7970FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36657 Genomic DNA Translation: AAA24936.1
PIRiA34950
RefSeqiNP_957531.1, NC_005324.1
WP_001043260.1, NZ_PJIY01000076.1
YP_002455756.1, NC_011795.1
YP_002891091.1, NC_012690.1
YP_002894395.1, NC_012692.1
YP_002995721.1, NC_012886.1
YP_006940094.1, NC_018995.1
YP_006952281.1, NC_019060.1
YP_006952747.1, NC_019065.1
YP_006952891.1, NC_019066.1
YP_006953258.1, NC_019070.1
YP_006953602.1, NC_019080.1
YP_007349479.1, NC_020086.1
YP_008574999.1, NC_022377.1
YP_009061414.1, NC_024978.1
YP_009061550.1, NC_024979.1
YP_009061670.1, NC_024980.1
YP_009068735.1, NC_025142.1
YP_009068851.1, NC_025143.1
YP_009069120.1, NC_025144.1
YP_009070858.1, NC_025176.1
YP_794145.1, NC_008490.1
YP_891140.1, NC_008597.1

Genome annotation databases

GeneIDi13877054
13905222
13905650
13905795
13906179
13906535
15152799
17035774
20468440
20468576
20468698
20491868
20491985
20492255
20492927
2716515
34760448
4397525
4525479
7205328
7872496
7872620
8094934

Similar proteinsi

Entry informationi

Entry nameiDHP2_ECOLX
AccessioniPrimary (citable) accession number: P0AC11
Secondary accession number(s): P19539
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid
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Main funding by: National Institutes of Health