Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Outer membrane protein assembly factor BamD

Gene

bamD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamA, the core component of the assembly machinery. Probably involved in transient protein interactions.UniRule annotation5 Publications

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
  • protein insertion into membrane Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7352-MONOMER.
ECOL316407:JW2577-MONOMER.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein assembly factor BamDUniRule annotation
Gene namesi
Name:bamDUniRule annotation
Synonyms:yfiO
Ordered Locus Names:b2595, JW2577
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14222. bamD.

Subcellular locationi

GO - Cellular componenti

  • Bam protein complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Depletion decreases the density of outer membrane proteins, but does not significantly affect transport of lipopolysaccharides to the outer membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919UniRule annotationAdd
BLAST
Chaini20 – 245226Outer membrane protein assembly factor BamDPRO_0000036226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteineCurated
Lipidationi20 – 201S-diacylglycerol cysteineCurated

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0AC02.
PaxDbiP0AC02.

Interactioni

Subunit structurei

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Forms a subcomplex with BamC and BamE. Interacts directly with BamA.UniRule annotation8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
atoAP764592EBI-1128087,EBI-1128061
bamAP0A94013EBI-1128087,EBI-907371
bamCP0A9034EBI-1128087,EBI-1129043

Protein-protein interaction databases

BioGridi4260615. 263 interactions.
851422. 1 interaction.
DIPiDIP-51120N.
IntActiP0AC02. 6 interactions.
MINTiMINT-8141303.
STRINGi511145.b2595.

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4514Combined sources
Helixi48 – 6114Combined sources
Helixi68 – 8114Combined sources
Helixi85 – 9814Combined sources
Beta strandi100 – 1023Combined sources
Helixi105 – 12016Combined sources
Turni126 – 1294Combined sources
Beta strandi133 – 1353Combined sources
Helixi138 – 15114Combined sources
Helixi160 – 18829Combined sources
Helixi191 – 20414Combined sources
Helixi209 – 22416Combined sources
Helixi228 – 24013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YHCX-ray1.80A29-245[»]
3Q5MX-ray2.60A23-245[»]
3TGOX-ray2.90A/B21-245[»]
5AYWX-ray3.56D20-245[»]
5D0OX-ray2.90D1-245[»]
5D0QX-ray3.50D/H1-245[»]
5EKQX-ray3.39D20-245[»]
ProteinModelPortaliP0AC02.
SMRiP0AC02. Positions 26-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal may interact with various proteins as a chaperone to assist in the folding and insertion of proteins into the outer membrane. The C-terminal region may serve as the link between BamA, BamC and BamE.1 Publication

Sequence similaritiesi

Belongs to the BamD family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106SNF. Bacteria.
COG4105. LUCA.
HOGENOMiHOG000260923.
InParanoidiP0AC02.
KOiK05807.
OMAiIHVADYY.
PhylomeDBiP0AC02.

Family and domain databases

CDDicd15830. BamD. 1 hit.
Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_00922. OM_assembly_BamD. 1 hit.
InterProiIPR017689. BamD.
IPR011990. TPR-like_helical_dom.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR03302. OM_YfiO. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AC02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRMKYLVAA ATLSLFLAGC SGSKEEVPDN PPNEIYATAQ QKLQDGNWRQ
60 70 80 90 100
AITQLEALDN RYPFGPYSQQ VQLDLIYAYY KNADLPLAQA AIDRFIRLNP
110 120 130 140 150
THPNIDYVMY MRGLTNMALD DSALQGFFGV DRSDRDPQHA RAAFSDFSKL
160 170 180 190 200
VRGYPNSQYT TDATKRLVFL KDRLAKYEYS VAEYYTERGA WVAVVNRVEG
210 220 230 240
MLRDYPDTQA TRDALPLMEN AYRQMQMNAQ AEKVAKIIAA NSSNT
Length:245
Mass (Da):27,829
Last modified:November 8, 2005 - v1
Checksum:i91D0A25E69248C49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591D → Y in CAA94434 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75644.1.
AP009048 Genomic DNA. Translation: BAA16480.1.
Z70523 Genomic DNA. Translation: CAA94434.1.
PIRiF65037.
RefSeqiNP_417086.1. NC_000913.3.
WP_000197686.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75644; AAC75644; b2595.
BAA16480; BAA16480; BAA16480.
GeneIDi947086.
KEGGiecj:JW2577.
eco:b2595.
PATRICi32120593. VBIEscCol129921_2695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75644.1.
AP009048 Genomic DNA. Translation: BAA16480.1.
Z70523 Genomic DNA. Translation: CAA94434.1.
PIRiF65037.
RefSeqiNP_417086.1. NC_000913.3.
WP_000197686.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YHCX-ray1.80A29-245[»]
3Q5MX-ray2.60A23-245[»]
3TGOX-ray2.90A/B21-245[»]
5AYWX-ray3.56D20-245[»]
5D0OX-ray2.90D1-245[»]
5D0QX-ray3.50D/H1-245[»]
5EKQX-ray3.39D20-245[»]
ProteinModelPortaliP0AC02.
SMRiP0AC02. Positions 26-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260615. 263 interactions.
851422. 1 interaction.
DIPiDIP-51120N.
IntActiP0AC02. 6 interactions.
MINTiMINT-8141303.
STRINGi511145.b2595.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Proteomic databases

EPDiP0AC02.
PaxDbiP0AC02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75644; AAC75644; b2595.
BAA16480; BAA16480; BAA16480.
GeneIDi947086.
KEGGiecj:JW2577.
eco:b2595.
PATRICi32120593. VBIEscCol129921_2695.

Organism-specific databases

EchoBASEiEB3974.
EcoGeneiEG14222. bamD.

Phylogenomic databases

eggNOGiENOG4106SNF. Bacteria.
COG4105. LUCA.
HOGENOMiHOG000260923.
InParanoidiP0AC02.
KOiK05807.
OMAiIHVADYY.
PhylomeDBiP0AC02.

Enzyme and pathway databases

BioCyciEcoCyc:G7352-MONOMER.
ECOL316407:JW2577-MONOMER.

Miscellaneous databases

PROiP0AC02.

Family and domain databases

CDDicd15830. BamD. 1 hit.
Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_00922. OM_assembly_BamD. 1 hit.
InterProiIPR017689. BamD.
IPR011990. TPR-like_helical_dom.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR03302. OM_YfiO. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAMD_ECOLI
AccessioniPrimary (citable) accession number: P0AC02
Secondary accession number(s): P77146, Q47344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.