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Protein

Fructoselysine 6-phosphate deglycase

Gene

frlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of fructoselysine 6-phosphate to glucose 6-phosphate and lysine.1 Publication

Catalytic activityi

Fructoselysine 6-phosphate + H2O = glucose 6-phosphate + L-lysine.

Pathwayi: fructoselysine degradation

This protein is involved in step 2 of the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Fructoselysine kinase (frlD)
  2. Fructoselysine 6-phosphate deglycase (frlB)
This subpathway is part of the pathway fructoselysine degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine, the pathway fructoselysine degradation and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G7723-MONOMER.
ECOL316407:JW5700-MONOMER.
MetaCyc:G7723-MONOMER.
UniPathwayiUPA00784; UER00770.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructoselysine 6-phosphate deglycase (EC:3.5.-.-)
Gene namesi
Name:frlB
Synonyms:yhfN
Ordered Locus Names:b3371, JW5700
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12909. frlB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Fructoselysine 6-phosphate deglycasePRO_0000136593Add
BLAST

Proteomic databases

PaxDbiP0AC00.

Interactioni

Subunit structurei

Homododecamer.Curated

Protein-protein interaction databases

BioGridi4262480. 197 interactions.
STRINGi511145.b3371.

Structurei

3D structure databases

ProteinModelPortaliP0AC00.
SMRiP0AC00. Positions 15-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 169135SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini201 – 331131SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107R1S. Bacteria.
COG2222. LUCA.
HOGENOMiHOG000005028.
InParanoidiP0AC00.
KOiK10708.
OMAiSKSIHAA.
OrthoDBiEOG6677NF.
PhylomeDBiP0AC00.

Family and domain databases

InterProiIPR001347. SIS.
[Graphical view]
PfamiPF01380. SIS. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDIDKSTVD FLVTENMVQE VEKVLSHDVP LVHAIVEEMV KRDIDRIYFV
60 70 80 90 100
ACGSPLNAAQ TAKHLADRFS DLQVYAISGW EFCDNTPYRL DDRCAVIGVS
110 120 130 140 150
DYGKTEEVIK ALELGRACGA LTAAFTKRAD SPITSAAEFS IDYQADCIWE
160 170 180 190 200
IHLLLCYSVV LEMITRLAPN AEIGKIKNDL KQLPNALGHL VRTWEEKGRQ
210 220 230 240 250
LGELASQWPM IYTVAAGPLR PLGYKEGIVT LMEFTWTHGC VIESGEFRHG
260 270 280 290 300
PLEIVEPGVP FLFLLGNDES RHTTERAINF VKQRTDNVIV IDYAEISQGL
310 320 330 340
HPWLAPFLMF VPMEWLCYYL SIYKDHNPDE RRYYGGLVEY
Length:340
Mass (Da):38,569
Last modified:November 8, 2005 - v1
Checksum:i503497DD301942C6
GO

Sequence cautioni

The sequence AAA58168.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58168.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76396.2.
AP009048 Genomic DNA. Translation: BAE77919.1.
PIRiF65131.
RefSeqiNP_417830.4. NC_000913.3.
WP_001295163.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76396; AAC76396; b3371.
BAE77919; BAE77919; BAE77919.
GeneIDi947875.
KEGGiecj:JW5700.
eco:b3371.
PATRICi32122176. VBIEscCol129921_3465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58168.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76396.2.
AP009048 Genomic DNA. Translation: BAE77919.1.
PIRiF65131.
RefSeqiNP_417830.4. NC_000913.3.
WP_001295163.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC00.
SMRiP0AC00. Positions 15-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262480. 197 interactions.
STRINGi511145.b3371.

Proteomic databases

PaxDbiP0AC00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76396; AAC76396; b3371.
BAE77919; BAE77919; BAE77919.
GeneIDi947875.
KEGGiecj:JW5700.
eco:b3371.
PATRICi32122176. VBIEscCol129921_3465.

Organism-specific databases

EchoBASEiEB2746.
EcoGeneiEG12909. frlB.

Phylogenomic databases

eggNOGiENOG4107R1S. Bacteria.
COG2222. LUCA.
HOGENOMiHOG000005028.
InParanoidiP0AC00.
KOiK10708.
OMAiSKSIHAA.
OrthoDBiEOG6677NF.
PhylomeDBiP0AC00.

Enzyme and pathway databases

UniPathwayiUPA00784; UER00770.
BioCyciEcoCyc:G7723-MONOMER.
ECOL316407:JW5700-MONOMER.
MetaCyc:G7723-MONOMER.

Miscellaneous databases

PROiP0AC00.

Family and domain databases

InterProiIPR001347. SIS.
[Graphical view]
PfamiPF01380. SIS. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Identification of a pathway for the utilization of the Amadori product fructoselysine in Escherichia coli."
    Wiame E., Delpierre G., Collard F., Van Schaftingen E.
    J. Biol. Chem. 277:42523-42529(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiFRLB_ECOLI
AccessioniPrimary (citable) accession number: P0AC00
Secondary accession number(s): P45540, Q2M737
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: January 20, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.