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Protein

Fructoselysine 6-phosphate deglycase

Gene

frlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible conversion of fructoselysine 6-phosphate to glucose 6-phosphate and lysine (PubMed:12147680). Functions in a fructoselysine degradation pathway that allows E.coli to grow on fructoselysine or psicoselysine (PubMed:14641112).2 Publications

Catalytic activityi

Fructoselysine 6-phosphate + H2O = glucose 6-phosphate + L-lysine.1 Publication

Enzyme regulationi

Strongly inhibited by ZnCl2.1 Publication

Kineticsi

  1. KM=0.4 mM for fructoselysine 6-phosphate1 Publication

    Pathwayi: fructoselysine degradation

    This protein is involved in step 2 of the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine.1 Publication1 Publication
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Fructoselysine 6-kinase (frlD)
    2. Fructoselysine 6-phosphate deglycase (frlB)
    This subpathway is part of the pathway fructoselysine degradation, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine, the pathway fructoselysine degradation and in Carbohydrate metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7723-MONOMER.
    MetaCyc:G7723-MONOMER.
    UniPathwayiUPA00784; UER00770.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructoselysine 6-phosphate deglycase1 Publication (EC:3.5.-.-1 Publication)
    Gene namesi
    Name:frlB1 Publication
    Synonyms:yhfN
    Ordered Locus Names:b3371, JW5700
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12909. frlB.

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are unable to grow on fructoselysine or psicoselysine, where they do grow on glucose.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001365931 – 340Fructoselysine 6-phosphate deglycaseAdd BLAST340

    Proteomic databases

    PaxDbiP0AC00.
    PRIDEiP0AC00.

    Expressioni

    Inductioni

    Induced by fructoselysine and psicoselysine. Makes part of the frl operon with FrlA, FrlC, FrlD and FrlR.2 Publications

    Interactioni

    Subunit structurei

    Homododecamer.1 Publication

    Protein-protein interaction databases

    BioGridi4262480. 197 interactors.
    STRINGi316385.ECDH10B_3547.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AC00.
    SMRiP0AC00.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini35 – 169SIS 1PROSITE-ProRule annotationAdd BLAST135
    Domaini201 – 331SIS 2PROSITE-ProRule annotationAdd BLAST131

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiENOG4107R1S. Bacteria.
    COG2222. LUCA.
    HOGENOMiHOG000005028.
    InParanoidiP0AC00.
    KOiK10708.
    PhylomeDBiP0AC00.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001347. SIS.
    PfamiView protein in Pfam
    PF01380. SIS. 1 hit.
    PROSITEiView protein in PROSITE
    PS51464. SIS. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P0AC00-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLDIDKSTVD FLVTENMVQE VEKVLSHDVP LVHAIVEEMV KRDIDRIYFV
    60 70 80 90 100
    ACGSPLNAAQ TAKHLADRFS DLQVYAISGW EFCDNTPYRL DDRCAVIGVS
    110 120 130 140 150
    DYGKTEEVIK ALELGRACGA LTAAFTKRAD SPITSAAEFS IDYQADCIWE
    160 170 180 190 200
    IHLLLCYSVV LEMITRLAPN AEIGKIKNDL KQLPNALGHL VRTWEEKGRQ
    210 220 230 240 250
    LGELASQWPM IYTVAAGPLR PLGYKEGIVT LMEFTWTHGC VIESGEFRHG
    260 270 280 290 300
    PLEIVEPGVP FLFLLGNDES RHTTERAINF VKQRTDNVIV IDYAEISQGL
    310 320 330 340
    HPWLAPFLMF VPMEWLCYYL SIYKDHNPDE RRYYGGLVEY
    Length:340
    Mass (Da):38,569
    Last modified:November 8, 2005 - v1
    Checksum:i503497DD301942C6
    GO

    Sequence cautioni

    The sequence AAA58168 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18997 Genomic DNA. Translation: AAA58168.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76396.2.
    AP009048 Genomic DNA. Translation: BAE77919.1.
    PIRiF65131.
    RefSeqiNP_417830.4. NC_000913.3.
    WP_001295163.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76396; AAC76396; b3371.
    BAE77919; BAE77919; BAE77919.
    GeneIDi947875.
    KEGGiecj:JW5700.
    eco:b3371.
    PATRICifig|1411691.4.peg.3358.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiFRLB_ECOLI
    AccessioniPrimary (citable) accession number: P0AC00
    Secondary accession number(s): P45540, Q2M737
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: July 5, 2017
    This is version 85 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways