Reviewed, UniProtKB/Swiss-Prot P0ABZ4 (KDSC_ECOLI)

Names and origin

Protein names

Recommended name:
    3-deoxy-D-manno-octulosonate 8-phosphate phosphatase
    EC=3.1.3.45
Alternative name(s):
    KDO 8-P phosphatase

Gene names

Name:	kdsC
Synonyms:	yrbI, yrbJ
Ordered Locus Names:	b3198, JW3165

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of KDO 8-P to KDO and inorganic phosphate.
Catalytic activity	3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate.
Cofactor	Magnesium Probable.
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 3/3. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the kdsC family.
biophysicochemical properties	pH dependence: Optimum pH is 5.5.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-manno-octulosonate-8-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase		PRO_0000201696

Sequences

	Sequence		Length	Mass (Da)
	P0ABZ4-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 75AF512CF4E5E6E2	FASTA	188	19,997
	10 20 30 40 50 60 MSKAGASLAT CYGPVSADVI AKAENIRLLI LDVDGVLSDG LIYMGNNGEE LKAFNVRDGY 70 80 90 100 110 120 GIRCALTSDI EVAIITGRKA KLVEDRCATL GITHLYQGQS NKLIAFSDLL EKLAIAPENV 130 140 150 160 170 180 AYVGDDLIDW PVMEKVGLSV AVADAHPLLI PRADYVTRIA GGRGAVREVC DLLLLAQGKL DEAKGQSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Escherichia coli YrbI is 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase." Wu J., Woodard R.W. J. Biol. Chem. 278:18117-18123(2003) [PubMed: 12639950] [Abstract] Cited for: CHARACTERIZATION. Strain: B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA57999.1. Frameshift. U18997 Genomic DNA. Translation: AAA58000.1. Frameshift. U00096 Genomic DNA. Translation: AAC76230.1. AP009048 Genomic DNA. Translation: BAE77242.1.
PIR	H65110.
RefSeq	AP_003741.1. NP_417665.1.
3D structure databases
HSSP	HSSP built from PDB template 1K1E based on UniProtKB P45314.
ModBase	Search...
Genome annotation databases
GeneID	947717.
GenomeReviews	Gene locus JW3165 in contig AP009048_GR. Gene locus b3198 in contig U00096_GR.
KEGG	ecj:JW3165. eco:b3198.
Organism-specific databases
EchoBASE	EB2656.
EcoGene	EG12804. kdsC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0ABZ4.
OMA	P0ABZ4. KTFNTLD.
Enzyme and pathway databases
BioCyc	EcoCyc:G7663-MON. MetaCyc:G7663-MON.
Family and domain databases
InterPro	IPR013200. HAD-SF_hydro-like_3. IPR006549. HAD-SF_hydro_IIIA. IPR010023. Phosphatase_KdsC. [Graphical view]
Pfam	PF08282. Hydrolase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01670. YrbI-phosphatas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSC_ECOLI

Accession

Primary (citable) accession number: P0ABZ4
Secondary accession number(s): P45396, P45398, Q2M914

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)