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Protein

Flagellar motor switch protein FliG

Gene

fliG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei160 – 1601Part of the EHPQR-motif

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • motor activity Source: InterPro

GO - Biological processi

  • bacterial-type flagellum assembly Source: EcoCyc
  • bacterial-type flagellum-dependent cell motility Source: EcoCyc
  • chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:FLIG-FLAGELLAR-SWITCH-PROTEIN.
ECOL316407:JW1923-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar motor switch protein FliG
Gene namesi
Name:fliG
Synonyms:fla AII.2, fla BII
Ordered Locus Names:b1939, JW1923
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11654. fliG.

Subcellular locationi

GO - Cellular componenti

  • bacterial-type flagellum basal body Source: UniProtKB-SubCell
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi252 – 2521Q → W: Decreases binding to YcgR, significantly improves motility in a yhjH disruption mutant. 1 Publication
Mutagenesisi284 – 2841D → W: Slightly improves motility in a yhjH disruption mutant. 1 Publication
Mutagenesisi292 – 2921N → W: Decreases binding to YcgR, significantly improves motility in a yhjH disruption mutant. 1 Publication
Mutagenesisi295 – 2951P → W: Decreases binding to YcgR, significantly improves motility in a yhjH disruption mutant. 1 Publication
Mutagenesisi298 – 2981L → W: Slightly improves motility in a yhjH disruption mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Flagellar motor switch protein FliGPRO_0000184089Add
BLAST

Proteomic databases

PaxDbiP0ABZ1.

Interactioni

Subunit structurei

Forms dimers and other homooligomers. Interacts with MotA via residues in its C-terminus. Interacts with flagellar brake protein YcgR.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1126524,EBI-1126524
fliFP257984EBI-1126524,EBI-1126492
fliMP0697416EBI-1126524,EBI-560439
ycgRP760103EBI-1126524,EBI-554507

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260384. 10 interactions.
DIPiDIP-408N.
IntActiP0ABZ1. 26 interactions.
MINTiMINT-8213365.
STRINGi511145.b1939.

Structurei

3D structure databases

ProteinModelPortaliP0ABZ1.
SMRiP0ABZ1. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi125 – 1284Part of the EHPQR-motif

Sequence similaritiesi

Belongs to the FliG family.Curated

Phylogenomic databases

eggNOGiENOG4105CS3. Bacteria.
COG1536. LUCA.
HOGENOMiHOG000257647.
InParanoidiP0ABZ1.
KOiK02410.
OMAiRNMSKRM.
PhylomeDBiP0ABZ1.

Family and domain databases

Gene3Di1.10.220.30. 3 hits.
InterProiIPR000090. Flg_Motor_Flig.
IPR023087. Flg_Motor_Flig_C.
IPR011002. FliG_a-hlx.
IPR032779. FliG_M.
IPR028263. FliG_N.
[Graphical view]
PANTHERiPTHR30534. PTHR30534. 1 hit.
PfamiPF01706. FliG_C. 1 hit.
PF14841. FliG_M. 1 hit.
PF14842. FliG_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003161. FliG. 1 hit.
PRINTSiPR00954. FLGMOTORFLIG.
SUPFAMiSSF48029. SSF48029. 2 hits.
TIGRFAMsiTIGR00207. fliG. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLTGTDKS VILLMTIGED RAAEVFKHLS QREVQTLSAA MANVTQISNK
60 70 80 90 100
QLTDVLAEFE QEAEQFAALN INANDYLRSV LVKALGEERA ASLLEDILET
110 120 130 140 150
RDTASGIETL NFMEPQSAAD LIRDEHPQII ATILVHLKRA QAADILALFD
160 170 180 190 200
ERLRHDVMLR IATFGGVQPA ALAELTEVLN GLLDGQNLKR SKMGGVRTAA
210 220 230 240 250
EIINLMKTQQ EEAVITAVRE FDGELAQKII DEMFLFENLV DVDDRSIQRL
260 270 280 290 300
LQEVDSESLL IALKGAEQPL REKFLRNMSQ RAADILRDDL ANRGPVRLSQ
310 320 330
VENEQKAILL IVRRLAETGE MVIGSGEDTY V
Length:331
Mass (Da):36,776
Last modified:October 25, 2005 - v1
Checksum:iE5CB4C0415C6E178
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 17012LRIAT…GVQPA → FVSHLWRRAPS in AAA75241 (PubMed:8224881).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13243 Genomic DNA. Translation: AAA75241.1.
U46011 Genomic DNA. Translation: AAB60186.1.
U00096 Genomic DNA. Translation: AAC75006.1.
AP009048 Genomic DNA. Translation: BAA15764.1.
D89826 Genomic DNA. Translation: BAA14030.1.
PIRiH64957.
RefSeqiNP_416449.1. NC_000913.3.
WP_000067950.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75006; AAC75006; b1939.
BAA15764; BAA15764; BAA15764.
GeneIDi946451.
KEGGiecj:JW1923.
eco:b1939.
PATRICi32119207. VBIEscCol129921_2018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13243 Genomic DNA. Translation: AAA75241.1.
U46011 Genomic DNA. Translation: AAB60186.1.
U00096 Genomic DNA. Translation: AAC75006.1.
AP009048 Genomic DNA. Translation: BAA15764.1.
D89826 Genomic DNA. Translation: BAA14030.1.
PIRiH64957.
RefSeqiNP_416449.1. NC_000913.3.
WP_000067950.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABZ1.
SMRiP0ABZ1. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260384. 10 interactions.
DIPiDIP-408N.
IntActiP0ABZ1. 26 interactions.
MINTiMINT-8213365.
STRINGi511145.b1939.

Proteomic databases

PaxDbiP0ABZ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75006; AAC75006; b1939.
BAA15764; BAA15764; BAA15764.
GeneIDi946451.
KEGGiecj:JW1923.
eco:b1939.
PATRICi32119207. VBIEscCol129921_2018.

Organism-specific databases

EchoBASEiEB1607.
EcoGeneiEG11654. fliG.

Phylogenomic databases

eggNOGiENOG4105CS3. Bacteria.
COG1536. LUCA.
HOGENOMiHOG000257647.
InParanoidiP0ABZ1.
KOiK02410.
OMAiRNMSKRM.
PhylomeDBiP0ABZ1.

Enzyme and pathway databases

BioCyciEcoCyc:FLIG-FLAGELLAR-SWITCH-PROTEIN.
ECOL316407:JW1923-MONOMER.

Miscellaneous databases

PROiP0ABZ1.

Family and domain databases

Gene3Di1.10.220.30. 3 hits.
InterProiIPR000090. Flg_Motor_Flig.
IPR023087. Flg_Motor_Flig_C.
IPR011002. FliG_a-hlx.
IPR032779. FliG_M.
IPR028263. FliG_N.
[Graphical view]
PANTHERiPTHR30534. PTHR30534. 1 hit.
PfamiPF01706. FliG_C. 1 hit.
PF14841. FliG_M. 1 hit.
PF14842. FliG_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003161. FliG. 1 hit.
PRINTSiPR00954. FLGMOTORFLIG.
SUPFAMiSSF48029. SSF48029. 2 hits.
TIGRFAMsiTIGR00207. fliG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFLIG_ECOLI
AccessioniPrimary (citable) accession number: P0ABZ1
Secondary accession number(s): P31067, P76915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.