Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit

Gene

hcaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively. This protein seems to be a 2Fe-2S ferredoxin.

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi44Iron-sulfur (2Fe-2S); via pros nitrogenSequence analysis1
Metal bindingi62Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi65Iron-sulfur (2Fe-2S); via pros nitrogenSequence analysis1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity Source: InterPro

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc

Keywordsi

Biological processAromatic hydrocarbons catabolism, Electron transport, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HCAC-MONOMER.
MetaCyc:HCAC-MONOMER.
BRENDAi3.7.1.14. 2026.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit
Gene namesi
Name:hcaC
Synonyms:hcaA3, phdB, yfhW
Ordered Locus Names:b2540, JW2524
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13458. hcaC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002016881 – 1063-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunitAdd BLAST106

Proteomic databases

PaxDbiP0ABW0.
PRIDEiP0ABW0.

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Protein-protein interaction databases

BioGridi4261575. 12 interactors.
IntActiP0ABW0. 4 interactors.
STRINGi316385.ECDH10B_2707.

Structurei

3D structure databases

ProteinModelPortaliP0ABW0.
SMRiP0ABW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 99RieskeAdd BLAST96

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105VQD. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128541.
InParanoidiP0ABW0.
KOiK05710.
PhylomeDBiP0ABW0.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01650. HcaC. 1 hit.
InterProiView protein in InterPro
IPR023739. HcaC.
IPR017941. Rieske_2Fe-2S.
PfamiView protein in Pfam
PF00355. Rieske. 1 hit.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiView protein in PROSITE
PS51296. RIESKE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIYACPVA DVPEGEALRI DTSPVIALFN VGGEFYAIND RCSHGNASMS
60 70 80 90 100
EGYLEDDATV ECPLHAASFC LKTGKALCLP ATDPLTTYPV HVEGGDIFID

LPEAQP
Length:106
Mass (Da):11,329
Last modified:October 25, 2005 - v1
Checksum:iB83149F4126BB72D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11070 Genomic DNA. Translation: CAA71950.1.
U00096 Genomic DNA. Translation: AAC75593.1.
AP009048 Genomic DNA. Translation: BAA16443.1.
PIRiC65031.
RefSeqiNP_417035.1. NC_000913.3.
WP_001080102.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75593; AAC75593; b2540.
BAA16443; BAA16443; BAA16443.
GeneIDi947015.
KEGGiecj:JW2524.
eco:b2540.
PATRICifig|1411691.4.peg.4194.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHCAC_ECOLI
AccessioniPrimary (citable) accession number: P0ABW0
Secondary accession number(s): O08099, P77266
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: July 5, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families