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Protein

3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit

Gene

hcaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively. This protein seems to be a 2Fe-2S ferredoxin.

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi44Iron-sulfur (2Fe-2S); via pros nitrogenSequence analysis1
Metal bindingi62Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi65Iron-sulfur (2Fe-2S); via pros nitrogenSequence analysis1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • 3-phenylpropionate dioxygenase activity Source: UniProtKB-HAMAP
  • iron-sulfur cluster binding Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc
  • aromatic compound catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HCAC-MONOMER.
ECOL316407:JW2524-MONOMER.
MetaCyc:HCAC-MONOMER.
BRENDAi3.7.1.14. 2026.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit
Gene namesi
Name:hcaC
Synonyms:hcaA3, phdB, yfhW
Ordered Locus Names:b2540, JW2524
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13458. hcaC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002016881 – 1063-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunitAdd BLAST106

Proteomic databases

PaxDbiP0ABW0.
PRIDEiP0ABW0.

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Protein-protein interaction databases

BioGridi4261575. 12 interactors.
IntActiP0ABW0. 4 interactors.
STRINGi511145.b2540.

Structurei

3D structure databases

ProteinModelPortaliP0ABW0.
SMRiP0ABW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 99RieskeAdd BLAST96

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Phylogenomic databases

eggNOGiENOG4105VQD. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128541.
InParanoidiP0ABW0.
KOiK05710.
OMAiCAIECWL.
PhylomeDBiP0ABW0.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01650. HcaC. 1 hit.
InterProiIPR023739. HcaC.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ABW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIYACPVA DVPEGEALRI DTSPVIALFN VGGEFYAIND RCSHGNASMS
60 70 80 90 100
EGYLEDDATV ECPLHAASFC LKTGKALCLP ATDPLTTYPV HVEGGDIFID

LPEAQP
Length:106
Mass (Da):11,329
Last modified:October 25, 2005 - v1
Checksum:iB83149F4126BB72D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11070 Genomic DNA. Translation: CAA71950.1.
U00096 Genomic DNA. Translation: AAC75593.1.
AP009048 Genomic DNA. Translation: BAA16443.1.
PIRiC65031.
RefSeqiNP_417035.1. NC_000913.3.
WP_001080102.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75593; AAC75593; b2540.
BAA16443; BAA16443; BAA16443.
GeneIDi947015.
KEGGiecj:JW2524.
eco:b2540.
PATRICi32120477. VBIEscCol129921_2641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11070 Genomic DNA. Translation: CAA71950.1.
U00096 Genomic DNA. Translation: AAC75593.1.
AP009048 Genomic DNA. Translation: BAA16443.1.
PIRiC65031.
RefSeqiNP_417035.1. NC_000913.3.
WP_001080102.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABW0.
SMRiP0ABW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261575. 12 interactors.
IntActiP0ABW0. 4 interactors.
STRINGi511145.b2540.

Proteomic databases

PaxDbiP0ABW0.
PRIDEiP0ABW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75593; AAC75593; b2540.
BAA16443; BAA16443; BAA16443.
GeneIDi947015.
KEGGiecj:JW2524.
eco:b2540.
PATRICi32120477. VBIEscCol129921_2641.

Organism-specific databases

EchoBASEiEB3231.
EcoGeneiEG13458. hcaC.

Phylogenomic databases

eggNOGiENOG4105VQD. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128541.
InParanoidiP0ABW0.
KOiK05710.
OMAiCAIECWL.
PhylomeDBiP0ABW0.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:HCAC-MONOMER.
ECOL316407:JW2524-MONOMER.
MetaCyc:HCAC-MONOMER.
BRENDAi3.7.1.14. 2026.

Miscellaneous databases

PROiP0ABW0.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01650. HcaC. 1 hit.
InterProiIPR023739. HcaC.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCAC_ECOLI
AccessioniPrimary (citable) accession number: P0ABW0
Secondary accession number(s): O08099, P77266
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.