Reviewed, UniProtKB/Swiss-Prot P0ABV2 (EXBD_ECOLI)

Names and origin

Protein names

Recommended name:
    Biopolymer transport protein exbD

Gene names

Name:	exbD
Ordered Locus Names:	b3005, JW2973

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the tonB-dependent energy-dependent transport of various receptor-bound substrates.
Subunit structure	The accessory proteins exbB and exbD seem to form a complex with tonB.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the exbD/tolR family.

Ontologies

Keywords
Biological process	Bacteriocin transport Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	bacteriocin transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 141

141

Biopolymer transport protein exbD

PRO_0000129117

Regions

Topological domain

1 – 22

22

Cytoplasmic Probable

Transmembrane

23 – 43

21

Signal-anchor for type II membrane protein Probable

Topological domain

44 – 141

98

Periplasmic Probable

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

141

Helix Strand Turn

Details...

Beta strand

65 – 69

5

Turn

70 – 72

3

Beta strand

73 – 76

4

Beta strand

79 – 81

3

Helix

86 – 93

8

Beta strand

94 – 96

3

Beta strand

102 – 106

5

Helix

112 – 124

13

Sequences

	Sequence		Length	Mass (Da)
	P0ABV2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 25A539A2FAEB9F6C	FASTA	141	15,527
	10 20 30 40 50 60 MAMHLNENLD DNGEMHDINV TPFIDVMLVL LIIFMVAAPL ATVDVKVNLP ASTSTPQPRP 70 80 90 100 110 120 EKPVYLSVKA DNSMFIGNDP VTDETMITAL NALTEGKKDT TIFFRADKTV DYETLMKVMD 130 140 TLHQAGYLKI GLVGEETAKA K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively." Eick-Helmerich K., Braun V. J. Bacteriol. 171:5117-5126(1989) [PubMed: 2670903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Membrane topology of the Escherichia coli ExbD protein." Kampfenkel K., Braun V. J. Bacteriol. 174:5485-5487(1992) [PubMed: 1644779] [Abstract] Cited for: TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M28819 Genomic DNA. Translation: AAA23733.1.
U28377 Genomic DNA. Translation: AAA69172.1.
U00096 Genomic DNA. Translation: AAC76041.1.
AP009048 Genomic DNA. Translation: BAE77063.1.

PIR

BVECED. JV0030.

RefSeq

AP_003557.1.
NP_417478.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PFU	NMR	-	A	43-141	[»]

ModBase

Search...

Protein family/group databases

TCDB

1.A.30.2.1. H+- or Na+-translocating bacterial flagellar motor 1ExbBD outer membrane transport energizer (Mot/Exb) superfamily.
2.C.1.1.1. TonB-ExbB-ExbD/TolA-TolQ-TolR (TonB) family of auxiliary proteins for energization of outer membrane receptor (OMR)-mediated active transport.

Genome annotation databases

GeneID

946345.

GenomeReviews

Gene locus JW2973 in contig AP009048_GR.
Gene locus b3005 in contig U00096_GR.

KEGG

ecj:JW2973.
eco:b3005.

Organism-specific databases

EchoBASE

EB0268.

EcoGene

EG10272. exbD.

CMR

Search...

Phylogenomic databases

HOGENOM

P0ABV2.

OMA

P0ABV2. DTRIFLR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10272-MON.

Family and domain databases

InterPro

IPR003400. ExbD.
IPR014170. TonB_ExbD_1.
[Graphical view]

Pfam

PF02472. ExbD. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02803. ExbD_1. 1 hit.

ProtoNet

Search...

Entry information

Entry name

EXBD_ECOLI

Accession

Primary (citable) accession number: P0ABV2
Secondary accession number(s): P18784, Q2M9J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)