P0ABU7 (EXBB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biopolymer transport protein exbB

Gene names

Name:	exbB
Ordered Locus Names:	b3006, JW2974

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the tonB-dependent energy-dependent transport of various receptor-bound substrates. Protects exbD from proteolytic degradation and functionally stabilizes tonB.
Subunit structure	The accessory proteins exbB and exbD seem to form a complex with tonB.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the exbB/tolQ family.

Ontologies

Keywords
Biological process	Bacteriocin transport Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	bacteriocin transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Biopolymer transport protein exbB		PRO_0000145799
Regions
	Topological domain	1 – 15	15	Periplasmic Probable
	Transmembrane	16 – 39	24	Helical; Probable
	Topological domain	40 – 127	88	Cytoplasmic Probable
	Transmembrane	128 – 155	28	Helical; Probable
	Topological domain	156 – 161	6	Periplasmic Probable
	Transmembrane	162 – 194	33	Helical; Probable
	Topological domain	195 – 244	50	Cytoplasmic Probable
Experimental info
	Sequence conflict	51	1	Q → L in AAA23732. Ref.1
	Sequence conflict	236	1	R → A in AAA23732. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0ABU7 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: CEB63893A80BA71F	FASTA	244	26,287
	10 20 30 40 50 60 MGNNLMQTDL SVWGMYQHAD IVVKCVMIGL ILASVVTWAI FFSKSVEFFN QKRRLKREQQ 70 80 90 100 110 120 LLAEARSLNQ ANDIAADFGS KSLSLHLLNE AQNELELSEG SDDNEGIKER TSFRLERRVA 130 140 150 160 170 180 AVGRQMGRGN GYLATIGAIS PFVGLFGTVW GIMNSFIGIA QTQTTNLAVV APGIAEALLA 190 200 210 220 230 240 TAIGLVAAIP AVVIYNVFAR QIGGFKAMLG DVAAQVLLLQ SRDLDLEASA AAHPVRVAQK LRAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively." Eick-Helmerich K., Braun V. J. Bacteriol. 171:5117-5126(1989) [PubMed: 2670903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli." Kampfenkel K., Braun V. J. Biol. Chem. 268:6050-6057(1993) [PubMed: 8449962] [Abstract] Cited for: TOPOLOGY.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28819 Genomic DNA. Translation: AAA23732.1. U28377 Genomic DNA. Translation: AAA69173.1. U00096 Genomic DNA. Translation: AAC76042.1. AP009048 Genomic DNA. Translation: BAE77064.1.
PIR	BVECXB. D65087.
RefSeq	NP_417479.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0ABU7.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47841N.
Protein family/group databases
TCDB	1.A.30.2.1. H+- or Na+-translocating bacterial flagellar motor/ExbBD outer membrane transport energizer (Mot/Exb) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003846; EBESCP00000003846; EBESCG00000003142. EBESCT00000018349; EBESCP00000017640; EBESCG00000017403.
GeneID	945420.
GenomeReviews	Gene locus JW2974 in contig AP009048_GR. Gene locus b3006 in contig U00096_GR.
KEGG	ecj:JW2974. eco:b3006.
PATRIC	32121428. VBIEscCol129921_3101.
Organism-specific databases
EchoBASE	EB0267.
EcoGene	EG10271. exbB.
Phylogenomic databases
eggNOG	COG0811.
GeneTree	EBGT00050000010126.
HOGENOM	HBG681852.
OMA	DDNNGIK.
PhylomeDB	P0ABU7.
ProtClustDB	PRK10414.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10271-MONOMER.
Gene expression databases
Genevestigator	P0ABU7.
Family and domain databases
InterPro	IPR002898. MotA_ExbB_proton_chnl. IPR014164. TonB_ExbB_1. [Graphical view]
KO	K03561.
Pfam	PF01618. MotA_ExbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR02797. ExbB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EXBB_ECOLI

Accession

Primary (citable) accession number: P0ABU7
Secondary accession number(s): P18783, Q2M9J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program