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Protein

Glyoxalase ElbB

Gene

elbB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays glyoxalase activity, catalyzing the conversion of glyoxal to glycolate (PubMed:26678554). However, this apparent glyoxalase activity may reflect a protein deglycase activity, which could be the primary function of this protein like other DJ-1 superfamily members such as PARK7, YajL, YhbO and HchA (Probable). Is not able to use methylglyoxal as subtrate (PubMed:26678554).Curated1 Publication

Catalytic activityi

Glycolate = glyoxal + H2O.1 Publication

Kineticsi

kcat is 0.48 min(-1) with glyoxal as substrate (at pH 7.4 and 37 degrees Celsius).1 Publication

  1. KM=1.21 mM for glyoxal (at pH 7.4 and 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei135 – 1351NucleophileBy similarity

    GO - Molecular functioni

    GO - Biological processi

    • isoprenoid biosynthetic process Source: EcoliWiki
    • positive regulation of isoprenoid metabolic process Source: EcoliWiki
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11383-MONOMER.
    ECOL316407:JW3176-MONOMER.

    Protein family/group databases

    MEROPSiC56.975.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyoxalase ElbB1 Publication (EC:4.2.1.-1 Publication)
    Alternative name(s):
    Sigma cross-reacting protein 27A1 Publication
    Short name:
    SCRP-27A1 Publication
    Gene namesi
    Name:elbB1 Publication
    Synonyms:elb21 Publication, yzzB
    Ordered Locus Names:b3209, JW3176
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11383. elbB.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217Glyoxalase ElbBPRO_0000201681Add
    BLAST

    Proteomic databases

    EPDiP0ABU5.
    PaxDbiP0ABU5.
    PRIDEiP0ABU5.

    2D gel databases

    SWISS-2DPAGEP0ABU5.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    BioGridi4262428. 8 interactions.
    DIPiDIP-48002N.
    IntActiP0ABU5. 6 interactions.
    STRINGi511145.b3209.

    Structurei

    Secondary structure

    1
    217
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75Combined sources
    Beta strandi10 – 123Combined sources
    Turni13 – 153Combined sources
    Helixi19 – 3113Combined sources
    Beta strandi35 – 406Combined sources
    Beta strandi42 – 443Combined sources
    Turni51 – 533Combined sources
    Helixi63 – 675Combined sources
    Turni68 – 736Combined sources
    Helixi78 – 803Combined sources
    Helixi83 – 853Combined sources
    Beta strandi87 – 915Combined sources
    Helixi96 – 994Combined sources
    Helixi104 – 1074Combined sources
    Helixi108 – 1103Combined sources
    Helixi115 – 12612Combined sources
    Beta strandi131 – 1344Combined sources
    Helixi137 – 1404Combined sources
    Helixi141 – 1444Combined sources
    Helixi157 – 1659Combined sources
    Beta strandi179 – 1813Combined sources
    Turni182 – 1854Combined sources
    Beta strandi186 – 1894Combined sources
    Helixi191 – 1933Combined sources
    Helixi198 – 21619Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OY1X-ray2.95A/B/C/D1-217[»]
    1VHQX-ray1.65A/B1-217[»]
    ProteinModelPortaliP0ABU5.
    SMRiP0ABU5. Positions 1-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ABU5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family.Curated

    Phylogenomic databases

    eggNOGiENOG4108556. Bacteria.
    COG3155. LUCA.
    HOGENOMiHOG000269874.
    InParanoidiP0ABU5.
    OMAiAQVQCFA.
    PhylomeDBiP0ABU5.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR026041. ElbB.
    [Graphical view]
    PIRSFiPIRSF006320. Elb2. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0ABU5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKIGVILSG CGVYDGSEIH EAVLTLLAIS RSGAQAVCFA PDKQQVDVIN
    60 70 80 90 100
    HLTGEAMTET RNVLIEAARI TRGEIRPLAQ ADAAELDALI VPGGFGAAKN
    110 120 130 140 150
    LSNFASLGSE CTVDRELKAL AQAMHQAGKP LGFMCIAPAM LPKIFDFPLR
    160 170 180 190 200
    LTIGTDIDTA EVLEEMGAEH VPCPVDDIVV DEDNKIVTTP AYMLAQNIAE
    210
    AASGIDKLVS RVLVLAE
    Length:217
    Mass (Da):22,982
    Last modified:October 25, 2005 - v1
    Checksum:i48A7957C29384DAC
    GO

    Sequence cautioni

    The sequence AAA58011 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111C → S in BAA02487 (Ref. 1) Curated
    Sequence conflicti195 – 21723AQNIA…LVLAE → RRTLQKRRAALISWFPACWF WLNE in BAA02487 (Ref. 1) CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D13188 Genomic DNA. Translation: BAA02487.1.
    U18997 Genomic DNA. Translation: AAA58011.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76241.2.
    AP009048 Genomic DNA. Translation: BAE77253.1.
    PIRiC65112.
    RefSeqiNP_417676.2. NC_000913.3.
    WP_001300411.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76241; AAC76241; b3209.
    BAE77253; BAE77253; BAE77253.
    GeneIDi947903.
    KEGGiecj:JW3176.
    eco:b3209.
    PATRICi32121838. VBIEscCol129921_3303.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D13188 Genomic DNA. Translation: BAA02487.1.
    U18997 Genomic DNA. Translation: AAA58011.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76241.2.
    AP009048 Genomic DNA. Translation: BAE77253.1.
    PIRiC65112.
    RefSeqiNP_417676.2. NC_000913.3.
    WP_001300411.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OY1X-ray2.95A/B/C/D1-217[»]
    1VHQX-ray1.65A/B1-217[»]
    ProteinModelPortaliP0ABU5.
    SMRiP0ABU5. Positions 1-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262428. 8 interactions.
    DIPiDIP-48002N.
    IntActiP0ABU5. 6 interactions.
    STRINGi511145.b3209.

    Protein family/group databases

    MEROPSiC56.975.

    2D gel databases

    SWISS-2DPAGEP0ABU5.

    Proteomic databases

    EPDiP0ABU5.
    PaxDbiP0ABU5.
    PRIDEiP0ABU5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76241; AAC76241; b3209.
    BAE77253; BAE77253; BAE77253.
    GeneIDi947903.
    KEGGiecj:JW3176.
    eco:b3209.
    PATRICi32121838. VBIEscCol129921_3303.

    Organism-specific databases

    EchoBASEiEB1356.
    EcoGeneiEG11383. elbB.

    Phylogenomic databases

    eggNOGiENOG4108556. Bacteria.
    COG3155. LUCA.
    HOGENOMiHOG000269874.
    InParanoidiP0ABU5.
    OMAiAQVQCFA.
    PhylomeDBiP0ABU5.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11383-MONOMER.
    ECOL316407:JW3176-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP0ABU5.
    PROiP0ABU5.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR026041. ElbB.
    [Graphical view]
    PIRSFiPIRSF006320. Elb2. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiELBB_ECOLI
    AccessioniPrimary (citable) accession number: P0ABU5
    Secondary accession number(s): P26428, P76673, Q2M903
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 25, 2005
    Last modified: September 7, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought (PubMed:9603997) to be involved in the early steps of isoprenoid biosynthesis.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.