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Protein

Ribosome-binding ATPase YchF

Gene

ychF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. Does not hydrolyze GTP.UniRule annotation1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

ATPase activity is stimulated by potassium and other monovalent cations. Stimulation is maximal in the presence of K+, followed by NH4+, Rb+, Na+ and Cs+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumBy similarity1
Metal bindingi36MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17ATPUniRule annotation6

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • enzyme inhibitor activity Source: EcoCyc
  • GTP binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • ribosomal large subunit binding Source: EcoCyc
  • ribosome binding Source: EcoCyc

GO - Biological processi

  • response to oxidative stress Source: EcoCyc

Keywordsi

LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11404-MONOMER.
MetaCyc:EG11404-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-binding ATPase YchFUniRule annotation
Gene namesi
Name:ychFUniRule annotation
Synonyms:engD, gtp1
Ordered Locus Names:b1203, JW1194
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11404. ychF.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12N → A: Shows similar hydrolysis activities in the presence of K(+) or Na(+). 1 Publication1
Mutagenesisi78K → A: Shows similar hydrolysis activities in the presence of K(+) or Na(+). 1 Publication1
Mutagenesisi78K → R: Retains K(+)-dependent stimulation of ATPase activity and differentiates between K(+) and Na(+). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002016752 – 363Ribosome-binding ATPase YchFAdd BLAST362

Proteomic databases

PaxDbiP0ABU2.
PRIDEiP0ABU2.

2D gel databases

SWISS-2DPAGEiP0ABU2.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4260106. 22 interactors.
IntActiP0ABU2. 6 interactors.
STRINGi316385.ECDH10B_1256.

Structurei

3D structure databases

ProteinModelPortaliP0ABU2.
SMRiP0ABU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 256OBG-type GAdd BLAST254

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3G. Bacteria.
COG0012. LUCA.
HOGENOMiHOG000087629.
InParanoidiP0ABU2.
KOiK06942.
PhylomeDBiP0ABU2.

Family and domain databases

Gene3Di1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
HAMAPiMF_00944. YchF_OLA1_ATPase. 1 hit.
InterProiView protein in InterPro
IPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR031167. G_OBG.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
PfamiView protein in Pfam
PF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
PIRSFiPIRSF006641. CHP00092. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00092. TIGR00092. 1 hit.
PROSITEiView protein in PROSITE
PS51710. G_OBG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NFPFCTIEPN TGVVPMPDPR
60 70 80 90 100
LDQLAEIVKP QRTLPTTMEF VDIAGLVKGA SKGEGLGNQF LTNIRETEAI
110 120 130 140 150
GHVVRCFEND NIIHVSGKVN PADDIEVINT ELALADLDTC ERAIHRVQKK
160 170 180 190 200
AKGGDKDAKA ELAVLEKCLP QLENAGMLRA LDLSAEEKAA IRYLSFLTLK
210 220 230 240 250
PTMYIANVNE DGFENNPYLD QVREIAAKEG SVVVPVCAAV EADIAELDDE
260 270 280 290 300
ERDEFMQELG LEEPGLNRVI RAGYKLLNLQ TYFTAGVKEV RAWTIPVGAT
310 320 330 340 350
APQAAGKIHT DFEKGFIRAQ TISFEDFITY KGEQGAKEAG KMRAEGKDYI
360
VKDGDVMNFL FNV
Length:363
Mass (Da):39,667
Last modified:January 23, 2007 - v2
Checksum:i9C2DAFFAC7586F53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74287.1.
AP009048 Genomic DNA. Translation: BAA36061.1.
X61941 Genomic DNA. Translation: CAA43946.1.
U02423 Genomic DNA. Translation: AAA67557.1.
PIRiH64866.
RefSeqiNP_415721.1. NC_000913.3.
WP_000505866.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74287; AAC74287; b1203.
BAA36061; BAA36061; BAA36061.
GeneIDi945769.
KEGGiecj:JW1194.
eco:b1203.
PATRICifig|1411691.4.peg.1081.

Similar proteinsi

Entry informationi

Entry nameiYCHF_ECOLI
AccessioniPrimary (citable) accession number: P0ABU2
Secondary accession number(s): P31216, P76018
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families