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Reviewed, UniProtKB/Swiss-Prot P0ABT5 (DUSB_ECOLI)

Last modified June 16, 2009. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    tRNA-dihydrouridine synthase B
    EC=1.-.-.-
Gene names
Name: dusB
Synonyms: yhdG
Ordered Locus Names: b3260, JW3228
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Ref.8

Cofactor

FAD By similarity.

Sequence similarities

Belongs to the dus family. DusB subfamily.

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Ontologies

Keywords
   Biological processtRNA processing
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionFAD binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction. Source: IntAct

tRNA dihydrouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-1124821,EBI-543750

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321tRNA-dihydrouridine synthase B
PRO_0000162084

Experimental info

Sequence conflict1311A → R in CAA44270. Ref.1
Sequence conflict2671H → D in CAA44270. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P0ABT5-1 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: B9A31AB459825DE5

FASTA32135,866
        10         20         30         40         50         60 
MRIGQYQLRN RLIAAPMAGI TDRPFRTLCY EMGAGLTVSE MMSSNPQVWE SDKSRLRMVH 

        70         80         90        100        110        120 
IDEPGIRTVQ IAGSDPKEMA DAARINVESG AQIIDINMGC PAKKVNRKLA GSALLQYPDV 

       130        140        150        160        170        180 
VKSILTEVVN AVDVPVTLKI RTGWAPEHRN CEEIAQLAED CGIQALTIHG RTRACLFNGE 

       190        200        210        220        230        240 
AEYDSIRAVK QKVSIPVIAN GDITDPLKAR AVLDYTGADA LMIGRAAQGR PWIFREIQHY 

       250        260        270        280        290        300 
LDTGELLPPL PLAEVKRLLC AHVRELHDFY GPAKGYRIAR KHVSWYLQEH APNDQFRRTF 

       310        320 
NAIEDASEQL EALEAYFENF A

« Hide

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References

« Hide 'large scale' references
[1]"The E.coli fis promoter is subject to stringent control and autoregulation."
Ninnemann O., Koch C., Kahmann R.
EMBO J. 11:1075-1083(1992) [PubMed: 1547773] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CSH50.
[2]"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
J. Bacteriol. 174:8043-8056(1992) [PubMed: 1459953] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12."
Vanet A., Plumbridge J.A., Alix J.-H.
J. Bacteriol. 175:7178-7188(1993) [PubMed: 8226664] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
[6]"Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene."
Koch C., Vanderkerckhove J., Kahmann R.
Proc. Natl. Acad. Sci. U.S.A. 85:4237-4241(1988) [PubMed: 2837762] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-321.
[7]"Isolation of the gene encoding the Hin recombinational enhancer binding protein."
Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed: 2835774] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 312-321.
Strain: K12.
[8]"Identification of the tRNA-dihydrouridine synthase family."
Bishop A.C., Xu J., Johnson R.C., Schimmel P., de Crecy-Lagard V.
J. Biol. Chem. 277:25090-25095(2002) [PubMed: 11983710] [Abstract]
Cited for: FUNCTION.
Strain: K12.

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Cross-references

Sequence databases

X62399 Genomic DNA. Translation: CAA44270.1.
M95784 Genomic DNA. Translation: AAA23782.1.
U18997 Genomic DNA. Translation: AAA58064.1.
U00096 Genomic DNA. Translation: AAC76292.1.
AP009048 Genomic DNA. Translation: BAE77301.1.
S67010 Genomic DNA. Translation: AAB28770.2.
J03816 Genomic DNA. No translation available.
J03245 Genomic DNA. Translation: AAA83855.1.
PIRB47043.
RefSeqAP_003800.1.
NP_417726.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP0ABT5. 4 interactions.

Genome annotation databases

GeneID947707.
GenomeReviewsGene locus JW3228 in contig AP009048_GR.
Gene locus b3260 in contig U00096_GR.
KEGGecj:JW3228.
eco:b3260.

Organism-specific databases

EchoBASEEB1287.
EcoGeneEG11311. dusB.
CMRSearch...

Phylogenomic databases

HOGENOMP0ABT5.
OMAP0ABT5. QLAGCEP.

Enzyme and pathway databases

BioCycEcoCyc:EG11311-MON.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR11082. Du_synth. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PIRSFPIRSF006621. Dus. 1 hit.
TIGRFAMsTIGR00737. nifR3_yhdG. 1 hit.
PROSITEPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDUSB_ECOLI
AccessionPrimary (citable) accession number: P0ABT5
Secondary accession number(s): P25717, Q2M8V5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents