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Protein

DNA polymerase III subunit theta

Gene

holE

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity (By similarity).By similarity
The exact function of the theta subunit is unknown.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciECOO157:HOLE-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit theta (EC:2.7.7.7)
Gene namesi
Name:holE
Ordered Locus Names:Z2891, ECs2552
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001055231 – 76DNA polymerase III subunit thetaAdd BLAST76

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1223775.
STRINGi155864.Z2891.

Structurei

3D structure databases

ProteinModelPortaliP0ABT0.
SMRiP0ABT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105MPK. Bacteria.
ENOG4111UZC. LUCA.
HOGENOMiHOG000219272.
KOiK02345.
OMAiFRERYNM.

Family and domain databases

Gene3Di1.10.1110.10. 1 hit.
InterProiIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamiPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46575. SSF46575. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS
60 70
WFRERLIAHR LASVNLSRLP YEPKLK
Length:76
Mass (Da):8,846
Last modified:October 25, 2005 - v1
Checksum:i3667D87327E96556
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56832.1.
BA000007 Genomic DNA. Translation: BAB35975.1.
PIRiD85796.
H90947.
RefSeqiNP_310579.1. NC_002695.1.
WP_000916763.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56832; AAG56832; Z2891.
BAB35975; BAB35975; BAB35975.
GeneIDi913059.
KEGGiece:Z2891.
ecs:ECs2552.
PATRICi18354474. VBIEscCol44059_2448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56832.1.
BA000007 Genomic DNA. Translation: BAB35975.1.
PIRiD85796.
H90947.
RefSeqiNP_310579.1. NC_002695.1.
WP_000916763.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0ABT0.
SMRiP0ABT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1223775.
STRINGi155864.Z2891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56832; AAG56832; Z2891.
BAB35975; BAB35975; BAB35975.
GeneIDi913059.
KEGGiece:Z2891.
ecs:ECs2552.
PATRICi18354474. VBIEscCol44059_2448.

Phylogenomic databases

eggNOGiENOG4105MPK. Bacteria.
ENOG4111UZC. LUCA.
HOGENOMiHOG000219272.
KOiK02345.
OMAiFRERYNM.

Enzyme and pathway databases

BioCyciECOO157:HOLE-MONOMER.

Family and domain databases

Gene3Di1.10.1110.10. 1 hit.
InterProiIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamiPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46575. SSF46575. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHOLE_ECO57
AccessioniPrimary (citable) accession number: P0ABT0
Secondary accession number(s): P28689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.