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P0ABS8

- HOLE_ECOLI

UniProt

P0ABS8 - HOLE_ECOLI

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Protein

DNA polymerase III subunit theta

Gene
holE, b1842, JW1831
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
The exact function of the theta subunit is unknown.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. protein binding Source: IntAct
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit theta (EC:2.7.7.7)
Gene namesi
Name:holE
Ordered Locus Names:b1842, JW1831
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11505. holE.

Subcellular locationi

GO - Cellular componenti

  1. DNA polymerase III, core complex Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676DNA polymerase III subunit thetaPRO_0000105522Add
BLAST

Proteomic databases

PaxDbiP0ABS8.
PRIDEiP0ABS8.

Expressioni

Gene expression databases

GenevestigatoriP0ABS8.

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaQP0300711EBI-549182,EBI-549131

Protein-protein interaction databases

DIPiDIP-39982N.
IntActiP0ABS8. 28 interactions.
MINTiMINT-1223798.
STRINGi511145.b1842.

Structurei

Secondary structure

1
76
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 84
Helixi11 – 2111
Beta strandi23 – 253
Helixi27 – 304
Helixi38 – 436
Helixi47 – 537
Helixi54 – 563
Beta strandi64 – 663
Beta strandi67 – 693

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DU2NMR-A1-76[»]
2AE9NMR-A1-76[»]
2AXDNMR-S1-76[»]
2XY8NMR-B1-76[»]
ProteinModelPortaliP0ABS8.
SMRiP0ABS8. Positions 1-76.

Miscellaneous databases

EvolutionaryTraceiP0ABS8.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG07474.
HOGENOMiHOG000219272.
KOiK02345.
OMAiDEMDKVN.
OrthoDBiEOG6BW53G.

Family and domain databases

Gene3Di1.10.1110.10. 1 hit.
InterProiIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamiPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46575. SSF46575. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABS8-1 [UniParc]FASTAAdd to Basket

« Hide

MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS   50
WFRERLIAHR LASVNLSRLP YEPKLK 76
Length:76
Mass (Da):8,846
Last modified:October 25, 2005 - v1
Checksum:i3667D87327E96556
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04572 Genomic DNA. Translation: AAA23697.1.
L05381 Genomic DNA. Translation: AAA23982.1.
U00096 Genomic DNA. Translation: AAC74912.1.
AP009048 Genomic DNA. Translation: BAA15650.1.
PIRiS34951.
RefSeqiNP_416356.1. NC_000913.3.
YP_490104.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74912; AAC74912; b1842.
BAA15650; BAA15650; BAA15650.
GeneIDi12931367.
947471.
KEGGiecj:Y75_p1818.
eco:b1842.
PATRICi32119005. VBIEscCol129921_1920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04572 Genomic DNA. Translation: AAA23697.1 .
L05381 Genomic DNA. Translation: AAA23982.1 .
U00096 Genomic DNA. Translation: AAC74912.1 .
AP009048 Genomic DNA. Translation: BAA15650.1 .
PIRi S34951.
RefSeqi NP_416356.1. NC_000913.3.
YP_490104.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DU2 NMR - A 1-76 [» ]
2AE9 NMR - A 1-76 [» ]
2AXD NMR - S 1-76 [» ]
2XY8 NMR - B 1-76 [» ]
ProteinModelPortali P0ABS8.
SMRi P0ABS8. Positions 1-76.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-39982N.
IntActi P0ABS8. 28 interactions.
MINTi MINT-1223798.
STRINGi 511145.b1842.

Proteomic databases

PaxDbi P0ABS8.
PRIDEi P0ABS8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74912 ; AAC74912 ; b1842 .
BAA15650 ; BAA15650 ; BAA15650 .
GeneIDi 12931367.
947471.
KEGGi ecj:Y75_p1818.
eco:b1842.
PATRICi 32119005. VBIEscCol129921_1920.

Organism-specific databases

EchoBASEi EB1468.
EcoGenei EG11505. holE.

Phylogenomic databases

eggNOGi NOG07474.
HOGENOMi HOG000219272.
KOi K02345.
OMAi DEMDKVN.
OrthoDBi EOG6BW53G.

Enzyme and pathway databases

BioCyci EcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ABS8.
PROi P0ABS8.

Gene expression databases

Genevestigatori P0ABS8.

Family and domain databases

Gene3Di 1.10.1110.10. 1 hit.
InterProi IPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view ]
Pfami PF06440. DNA_pol3_theta. 1 hit.
[Graphical view ]
ProDomi PD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF46575. SSF46575. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA polymerase III accessory proteins. V. Theta encoded by holE."
    Studwell-Vaughan P.S., O'Donnell M.
    J. Biol. Chem. 268:11785-11791(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme."
    Carter J.R., Franden M.A., Aebersold R.H., Kim D.R., McHenry C.S.
    Nucleic Acids Res. 21:3281-3286(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23; 44-49 AND 69-73.
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
    O'Donnell M.
    Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiHOLE_ECOLI
AccessioniPrimary (citable) accession number: P0ABS8
Secondary accession number(s): P28689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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