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P0ABS8 (HOLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit theta

EC=2.7.7.7
Gene names
Name:holE
Ordered Locus Names:b1842, JW1831
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length76 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

The exact function of the theta subunit is unknown.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactions

With

Entry

#Exp.

IntAct

Notes

dnaQP0300711EBI-549182,EBI-549131

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7676DNA polymerase III subunit theta
PRO_0000105522

Secondary structure

................. 76
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ABS8 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 3667D87327E96556

FASTA768,846
        10         20         30         40         50         60 
MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS WFRERLIAHR 

        70 
LASVNLSRLP YEPKLK 

« Hide

References

« Hide 'large scale' references
[1]"DNA polymerase III accessory proteins. V. Theta encoded by holE."
Studwell-Vaughan P.S., O'Donnell M.
J. Biol. Chem. 268:11785-11791(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme."
Carter J.R., Franden M.A., Aebersold R.H., Kim D.R., McHenry C.S.
Nucleic Acids Res. 21:3281-3286(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23; 44-49 AND 69-73.
Strain: K12 / MG1655 / ATCC 47076.
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
O'Donnell M.
Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04572 Genomic DNA. Translation: AAA23697.1.
L05381 Genomic DNA. Translation: AAA23982.1.
U00096 Genomic DNA. Translation: AAC74912.1.
AP009048 Genomic DNA. Translation: BAA15650.1.
PIRS34951.
RefSeqNP_416356.1. NC_000913.3.
YP_490104.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DU2NMR-A1-76[»]
2AE9NMR-A1-76[»]
2AXDNMR-S1-76[»]
2XY8NMR-B1-76[»]
ProteinModelPortalP0ABS8.
SMRP0ABS8. Positions 1-76.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-39982N.
IntActP0ABS8. 28 interactions.
MINTMINT-1223798.
STRING511145.b1842.

Proteomic databases

PaxDbP0ABS8.
PRIDEP0ABS8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74912; AAC74912; b1842.
BAA15650; BAA15650; BAA15650.
GeneID12931367.
947471.
KEGGecj:Y75_p1818.
eco:b1842.
PATRIC32119005. VBIEscCol129921_1920.

Organism-specific databases

EchoBASEEB1468.
EcoGeneEG11505. holE.

Phylogenomic databases

eggNOGNOG07474.
HOGENOMHOG000219272.
KOK02345.
OMADEMDKVN.
OrthoDBEOG6BW53G.

Enzyme and pathway databases

BioCycEcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.

Gene expression databases

GenevestigatorP0ABS8.

Family and domain databases

Gene3D1.10.1110.10. 1 hit.
InterProIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF46575. SSF46575. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0ABS8.
PROP0ABS8.

Entry information

Entry nameHOLE_ECOLI
AccessionPrimary (citable) accession number: P0ABS8
Secondary accession number(s): P28689
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene