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P0ABS8

- HOLE_ECOLI

UniProt

P0ABS8 - HOLE_ECOLI

Protein

DNA polymerase III subunit theta

Gene

holE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
    The exact function of the theta subunit is unknown.

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. protein binding Source: IntAct

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11505-MONOMER.
    ECOL316407:JW1831-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit theta (EC:2.7.7.7)
    Gene namesi
    Name:holE
    Ordered Locus Names:b1842, JW1831
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11505. holE.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA polymerase III, core complex Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676DNA polymerase III subunit thetaPRO_0000105522Add
    BLAST

    Proteomic databases

    PaxDbiP0ABS8.
    PRIDEiP0ABS8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ABS8.

    Interactioni

    Subunit structurei

    The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    dnaQP0300711EBI-549182,EBI-549131

    Protein-protein interaction databases

    DIPiDIP-39982N.
    IntActiP0ABS8. 28 interactions.
    MINTiMINT-1223798.
    STRINGi511145.b1842.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84
    Helixi11 – 2111
    Beta strandi23 – 253
    Helixi27 – 304
    Helixi38 – 436
    Helixi47 – 537
    Helixi54 – 563
    Beta strandi64 – 663
    Beta strandi67 – 693

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DU2NMR-A1-76[»]
    2AE9NMR-A1-76[»]
    2AXDNMR-S1-76[»]
    2XY8NMR-B1-76[»]
    ProteinModelPortaliP0ABS8.
    SMRiP0ABS8. Positions 1-76.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ABS8.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG07474.
    HOGENOMiHOG000219272.
    KOiK02345.
    OMAiDEMDKVN.
    OrthoDBiEOG6BW53G.

    Family and domain databases

    Gene3Di1.10.1110.10. 1 hit.
    InterProiIPR009052. DNA_pol_III_theta_bac.
    IPR011325. DNA_pol_III_theta_core_bac.
    [Graphical view]
    PfamiPF06440. DNA_pol3_theta. 1 hit.
    [Graphical view]
    ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF46575. SSF46575. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0ABS8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS   50
    WFRERLIAHR LASVNLSRLP YEPKLK 76
    Length:76
    Mass (Da):8,846
    Last modified:October 25, 2005 - v1
    Checksum:i3667D87327E96556
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04572 Genomic DNA. Translation: AAA23697.1.
    L05381 Genomic DNA. Translation: AAA23982.1.
    U00096 Genomic DNA. Translation: AAC74912.1.
    AP009048 Genomic DNA. Translation: BAA15650.1.
    PIRiS34951.
    RefSeqiNP_416356.1. NC_000913.3.
    YP_490104.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74912; AAC74912; b1842.
    BAA15650; BAA15650; BAA15650.
    GeneIDi12931367.
    947471.
    KEGGiecj:Y75_p1818.
    eco:b1842.
    PATRICi32119005. VBIEscCol129921_1920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04572 Genomic DNA. Translation: AAA23697.1 .
    L05381 Genomic DNA. Translation: AAA23982.1 .
    U00096 Genomic DNA. Translation: AAC74912.1 .
    AP009048 Genomic DNA. Translation: BAA15650.1 .
    PIRi S34951.
    RefSeqi NP_416356.1. NC_000913.3.
    YP_490104.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DU2 NMR - A 1-76 [» ]
    2AE9 NMR - A 1-76 [» ]
    2AXD NMR - S 1-76 [» ]
    2XY8 NMR - B 1-76 [» ]
    ProteinModelPortali P0ABS8.
    SMRi P0ABS8. Positions 1-76.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-39982N.
    IntActi P0ABS8. 28 interactions.
    MINTi MINT-1223798.
    STRINGi 511145.b1842.

    Proteomic databases

    PaxDbi P0ABS8.
    PRIDEi P0ABS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74912 ; AAC74912 ; b1842 .
    BAA15650 ; BAA15650 ; BAA15650 .
    GeneIDi 12931367.
    947471.
    KEGGi ecj:Y75_p1818.
    eco:b1842.
    PATRICi 32119005. VBIEscCol129921_1920.

    Organism-specific databases

    EchoBASEi EB1468.
    EcoGenei EG11505. holE.

    Phylogenomic databases

    eggNOGi NOG07474.
    HOGENOMi HOG000219272.
    KOi K02345.
    OMAi DEMDKVN.
    OrthoDBi EOG6BW53G.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11505-MONOMER.
    ECOL316407:JW1831-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0ABS8.
    PROi P0ABS8.

    Gene expression databases

    Genevestigatori P0ABS8.

    Family and domain databases

    Gene3Di 1.10.1110.10. 1 hit.
    InterProi IPR009052. DNA_pol_III_theta_bac.
    IPR011325. DNA_pol_III_theta_core_bac.
    [Graphical view ]
    Pfami PF06440. DNA_pol3_theta. 1 hit.
    [Graphical view ]
    ProDomi PD057609. DNA_pol_III_theta_bac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF46575. SSF46575. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "DNA polymerase III accessory proteins. V. Theta encoded by holE."
      Studwell-Vaughan P.S., O'Donnell M.
      J. Biol. Chem. 268:11785-11791(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme."
      Carter J.R., Franden M.A., Aebersold R.H., Kim D.R., McHenry C.S.
      Nucleic Acids Res. 21:3281-3286(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23; 44-49 AND 69-73.
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
      O'Donnell M.
      Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiHOLE_ECOLI
    AccessioniPrimary (citable) accession number: P0ABS8
    Secondary accession number(s): P28689
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 25, 2005
    Last modified: June 11, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3