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Protein

DNA polymerase III subunit theta

Gene

holE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
The exact function of the theta subunit is unknown.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.
MetaCyc:EG11505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit theta (EC:2.7.7.7)
Gene namesi
Name:holE
Ordered Locus Names:b1842, JW1831
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11505. holE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • DNA polymerase III, core complex Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001055221 – 76DNA polymerase III subunit thetaAdd BLAST76

Proteomic databases

PaxDbiP0ABS8.
PRIDEiP0ABS8.

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaQP0300711EBI-549182,EBI-549131

Protein-protein interaction databases

BioGridi4260361. 41 interactors.
DIPiDIP-39982N.
IntActiP0ABS8. 28 interactors.
MINTiMINT-1223798.
STRINGi511145.b1842.

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Helixi11 – 21Combined sources11
Beta strandi23 – 25Combined sources3
Helixi27 – 30Combined sources4
Helixi38 – 43Combined sources6
Helixi47 – 53Combined sources7
Helixi54 – 56Combined sources3
Beta strandi64 – 66Combined sources3
Beta strandi67 – 69Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DU2NMR-A1-76[»]
2AE9NMR-A1-76[»]
2AXDNMR-S1-76[»]
2XY8NMR-B1-76[»]
ProteinModelPortaliP0ABS8.
SMRiP0ABS8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABS8.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105MPK. Bacteria.
ENOG4111UZC. LUCA.
HOGENOMiHOG000219272.
KOiK02345.
OMAiFRERYNM.

Family and domain databases

Gene3Di1.10.1110.10. 1 hit.
InterProiIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamiPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46575. SSF46575. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS
60 70
WFRERLIAHR LASVNLSRLP YEPKLK
Length:76
Mass (Da):8,846
Last modified:October 25, 2005 - v1
Checksum:i3667D87327E96556
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04572 Genomic DNA. Translation: AAA23697.1.
L05381 Genomic DNA. Translation: AAA23982.1.
U00096 Genomic DNA. Translation: AAC74912.1.
AP009048 Genomic DNA. Translation: BAA15650.1.
PIRiS34951.
RefSeqiNP_416356.1. NC_000913.3.
WP_000916763.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74912; AAC74912; b1842.
BAA15650; BAA15650; BAA15650.
GeneIDi947471.
KEGGiecj:JW1831.
eco:b1842.
PATRICi32119005. VBIEscCol129921_1920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04572 Genomic DNA. Translation: AAA23697.1.
L05381 Genomic DNA. Translation: AAA23982.1.
U00096 Genomic DNA. Translation: AAC74912.1.
AP009048 Genomic DNA. Translation: BAA15650.1.
PIRiS34951.
RefSeqiNP_416356.1. NC_000913.3.
WP_000916763.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DU2NMR-A1-76[»]
2AE9NMR-A1-76[»]
2AXDNMR-S1-76[»]
2XY8NMR-B1-76[»]
ProteinModelPortaliP0ABS8.
SMRiP0ABS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260361. 41 interactors.
DIPiDIP-39982N.
IntActiP0ABS8. 28 interactors.
MINTiMINT-1223798.
STRINGi511145.b1842.

Proteomic databases

PaxDbiP0ABS8.
PRIDEiP0ABS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74912; AAC74912; b1842.
BAA15650; BAA15650; BAA15650.
GeneIDi947471.
KEGGiecj:JW1831.
eco:b1842.
PATRICi32119005. VBIEscCol129921_1920.

Organism-specific databases

EchoBASEiEB1468.
EcoGeneiEG11505. holE.

Phylogenomic databases

eggNOGiENOG4105MPK. Bacteria.
ENOG4111UZC. LUCA.
HOGENOMiHOG000219272.
KOiK02345.
OMAiFRERYNM.

Enzyme and pathway databases

BioCyciEcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.
MetaCyc:EG11505-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ABS8.
PROiP0ABS8.

Family and domain databases

Gene3Di1.10.1110.10. 1 hit.
InterProiIPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]
PfamiPF06440. DNA_pol3_theta. 1 hit.
[Graphical view]
ProDomiPD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46575. SSF46575. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHOLE_ECOLI
AccessioniPrimary (citable) accession number: P0ABS8
Secondary accession number(s): P28689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.