DNA polymerase III subunit theta - Escherichia coli (strain K12)

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P0ABS8 (HOLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit theta
EC=2.7.7.7

Gene names

Name:	holE
Ordered Locus Names:	b1842, JW1831

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

The exact function of the theta subunit is unknown.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Ontologies

Keywords
Biological process	DNA replication
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaQ	P03007	6	EBI-549182,EBI-549131

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 76

DNA polymerase III subunit theta

PRO_0000105522

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ABS8 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 3667D87327E96556
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FASTA

8,846

        10         20         30         40         50         60 
MLKNLAKLDQ TEMDKVNVDL AAAGVAFKER YNMPVIAEAV EREQPEHLRS WFRERLIAHR 

        70 
LASVNLSRLP YEPKLK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA polymerase III accessory proteins. V. Theta encoded by holE." Studwell-Vaughan P.S., O'Donnell M. J. Biol. Chem. 268:11785-11791(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme." Carter J.R., Franden M.A., Aebersold R.H., Kim D.R., McHenry C.S. Nucleic Acids Res. 21:3281-3286(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23; 44-49 AND 69-73. Strain: K12 / MG1655 / ATCC 47076.
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Accessory protein function in the DNA polymerase III holoenzyme from E. coli." O'Donnell M. Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L04572 Genomic DNA. Translation: AAA23697.1.
L05381 Genomic DNA. Translation: AAA23982.1.
U00096 Genomic DNA. Translation: AAC74912.1.
AP009048 Genomic DNA. Translation: BAA15650.1.

PIR

S34951.

RefSeq

NP_416356.1. NC_000913.2.
YP_490104.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DU2	NMR	-	A	1-76	[»]
2AE9	NMR	-	A	1-76	[»]
2AXD	NMR	-	S	1-76	[»]
2XY8	NMR	-	B	1-76	[»]

ProteinModelPortal

P0ABS8.

SMR

P0ABS8. Positions 1-76.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-39982N.

IntAct

P0ABS8. 28 interactions.

MINT

MINT-1223798.

STRING

511145.b1842.

Proteomic databases

PaxDb

P0ABS8.

PRIDE

P0ABS8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74912; AAC74912; b1842.
BAA15650; BAA15650; BAA15650.

GeneID

12931367.
947471.

KEGG

ecj:Y75_p1818.
eco:b1842.

PATRIC

32119005. VBIEscCol129921_1920.

Organism-specific databases

EchoBASE

EB1468.

EcoGene

EG11505. holE.

Phylogenomic databases

eggNOG

NOG07474.

HOGENOM

HOG000219272.

K02345.

OMA

LPYEPKS.

ProtClustDB

PRK10969.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11505-MONOMER.
ECOL316407:JW1831-MONOMER.

Gene expression databases

Genevestigator

P0ABS8.

Family and domain databases

Gene3D

1.10.1110.10. 1 hit.

InterPro

IPR009052. DNA_pol_III_theta_bac.
IPR011325. DNA_pol_III_theta_core_bac.
[Graphical view]

Pfam

PF06440. DNA_pol3_theta. 1 hit.
[Graphical view]

ProDom

PD057609. DNA_pol_III_theta_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF46575. DNA_pol_II_beta. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0ABS8.

Entry information

Entry name

HOLE_ECOLI

Accession

Primary (citable) accession number: P0ABS8
Secondary accession number(s): P28689

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents