Reviewed, UniProtKB/Swiss-Prot P0ABS1 (DKSA_ECOLI)

Names and origin

Protein names

Recommended name:
    DnaK suppressor protein

Gene names

Name:	dksA
Ordered Locus Names:	b0145, JW0141

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed.
Sequence similarities	Contains 1 dksA C4-type zinc finger.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: UniProtKB
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 151

151

DnaK suppressor protein

PRO_0000187536

Regions

Zinc finger

114 – 138

25

dksA C4-type

Experimental info

Sequence conflict

4

1

G → Q AA sequence Ref.6

Sequence conflict

94 – 95

2

KL → NV in AAA23687. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

151

Helix Strand Turn

Details...

Helix

13 – 17

5

Helix

34 – 67

34

Helix

72 – 74

3

Helix

75 – 107

33

Beta strand

115 – 118

4

Helix

123 – 128

6

Helix

136 – 150

15

Sequences

	Sequence		Length	Mass (Da)
	P0ABS1-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 620842DB15E066A9	FASTA	151	17,528
	10 20 30 40 50 60 MQEGQNRKTS SLSILAIAGV EPYQEKPGEE YMNEAQLAHF RRILEAWRNQ LRDEVDRTVT 70 80 90 100 110 120 HMQDEAANFP DPVDRAAQEE EFSLELRNRD RERKLIKKIE KTLKKVEDED FGYCESCGVE 130 140 150 IGIRRLEARP TADLCIDCKT LAEIREKQMA G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation." Kang P.J., Craig E.A. J. Bacteriol. 172:2055-2064(1990) [PubMed: 2180916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli." Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T. J. Bacteriol. 173:2644-2648(1991) [PubMed: 2013578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 1-8. Strain: K12 / EMG2.
[7]	"Protein identification with N and C-terminal sequence tags in proteome projects." Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F. J. Mol. Biol. 278:599-608(1998) [PubMed: 9600841] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-4. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M34945 Genomic DNA. Translation: AAA23687.1.
U00096 Genomic DNA. Translation: AAC73256.1.
AP009048 Genomic DNA. Translation: BAB96722.1.
M60726 Genomic DNA. Translation: AAA72978.1.

PIR

S45214.

RefSeq

AP_000806.1.
NP_414687.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TJL	X-ray	2.00	A/B/C/D/E/F/G/H/I/J	1-151	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0ABS1. 9 interactions.

2-D gel databases

SWISS-2DPAGE

P0ABS1.

Genome annotation databases

GeneID

944850.

GenomeReviews

Gene locus JW0141 in contig AP009048_GR.
Gene locus b0145 in contig U00096_GR.

KEGG

ecj:JW0141.
eco:b0145.

Organism-specific databases

EchoBASE

EB0226.

EcoGene

EG10230. dksA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0ABS1.

OMA

P0ABS1. GEEYMND.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10230-MON.

Family and domain databases

InterPro

IPR012784. DksA_RNA_pol_bd.
IPR000962. Znf_DskA_TraR.
[Graphical view]

Pfam

PF01258. zf-dskA_traR. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02420. dksA. 1 hit.

PROSITE

PS01102. ZF_DKSA_1. 1 hit.
PS51128. ZF_DKSA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DKSA_ECOLI

Accession

Primary (citable) accession number: P0ABS1
Secondary accession number(s): P18274

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)