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Protein

Carnitine monooxygenase oxygenase subunit

Gene

yeaW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts carnitine to trimethylamine and malic semialdehyde. Can also use gamma-butyrobetaine, choline and betaine as substrates.1 Publication

Catalytic activityi

L-carnitine + NAD(P)H + H+ + O2 = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)+ + H2O.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.UniRule annotation
  • Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi91Iron-sulfur (2Fe-2S); via pros nitrogenUniRule annotation1
Metal bindingi109Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi112Iron-sulfur (2Fe-2S); via pros nitrogenUniRule annotation1
Metal bindingi211Iron; via tele nitrogenUniRule annotation1
Metal bindingi216Iron; via tele nitrogenUniRule annotation1
Metal bindingi325IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6988-MONOMER
MetaCyc:G6988-MONOMER
UniPathwayiUPA00117

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine monooxygenase oxygenase subunitUniRule annotationCurated (EC:1.14.13.-UniRule annotation1 Publication)
Alternative name(s):
Carnitine monooxygenase alpha subunitUniRule annotationCurated
Gene namesi
Name:yeaW
Ordered Locus Names:b1802, JW5294
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13509 yeaW

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850631 – 374Carnitine monooxygenase oxygenase subunitAdd BLAST374

Proteomic databases

PaxDbiP0ABR7
PRIDEiP0ABR7

Interactioni

Subunit structurei

Composed of an oxygenase subunit (yeaW) and a reductase subunit (yeaX).By similarity

Protein-protein interaction databases

BioGridi4259149, 7 interactors
STRINGi316385.ECDH10B_1940

Structurei

3D structure databases

ProteinModelPortaliP0ABR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 155RieskeUniRule annotationAdd BLAST109

Sequence similaritiesi

Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. CntA subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105DVW Bacteria
COG4638 LUCA
HOGENOMiHOG000248945
InParanoidiP0ABR7
KOiK22443
OMAiTNVVEFY
PhylomeDBiP0ABR7

Family and domain databases

Gene3Di2.102.10.10, 1 hit
HAMAPiMF_02097 Carnitine_monoox_A, 1 hit
InterProiView protein in InterPro
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR015881 Ring-hydroxy_dOase_2Fe2S_BS
IPR015879 Ring_hydroxy_dOase_asu_C_dom
IPR001663 Rng_hydr_dOase-A
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF00848 Ring_hydroxyl_A, 1 hit
PRINTSiPR00090 RNGDIOXGNASE
SUPFAMiSSF50022 SSF50022, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit
PS00570 RING_HYDROXYL_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLSPDFVL PENFCANPQE AWTIPARFYT DQNAFEHEKE NVFAKSWICV
60 70 80 90 100
AHSSELANAN DYVTREIIGE SIVLVRGRDK VLRAFYNVCP HRGHQLLSGE
110 120 130 140 150
GKAKNVITCP YHAWAFKLDG NLAHARNCEN VANFDSDKAQ LVPVRLEEYA
160 170 180 190 200
GFVFINMDPN ATSVEDQLPG LGAKVLEACP EVHDLKLAAR FTTRTPANWK
210 220 230 240 250
NIVDNYLECY HCGPAHPGFS DSVQVDRYWH TMHGNWTLQY GFAKPSEQSF
260 270 280 290 300
KFEEGTDAAF HGFWLWPCTM LNVTPIKGMM TVIYEFPVDS ETTLQNYDIY
310 320 330 340 350
FTNEELTDEQ KSLIEWYRDV FRPEDLRLVE SVQKGLKSRG YRGQGRIMAD
360 370
SSGSGISEHG IAHFHNLLAQ VFKD
Length:374
Mass (Da):42,561
Last modified:November 8, 2005 - v1
Checksum:iBB5386ACA9585606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74872.1
AP009048 Genomic DNA Translation: BAA15597.2
PIRiB64941
RefSeqiNP_416316.1, NC_000913.3
WP_000067822.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74872; AAC74872; b1802
BAA15597; BAA15597; BAA15597
GeneIDi946325
KEGGiecj:JW5294
eco:b1802
PATRICifig|1411691.4.peg.451

Similar proteinsi

Entry informationi

Entry nameiCNTA_ECOLI
AccessioniPrimary (citable) accession number: P0ABR7
Secondary accession number(s): P76253
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health