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Protein

3-phenylpropionate/cinnamic acid dioxygenase subunit alpha

Gene

hcaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.1 Publication

Catalytic activityi

3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+.
(2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi87Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1
Metal bindingi105Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi108Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1
Metal bindingi213IronBy similarity1
Metal bindingi218IronBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc
  • aromatic compound catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENYLPRODIOXY-MONOMER.
ECOL316407:JW2522-MONOMER.
MetaCyc:PHENYLPRODIOXY-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (EC:1.14.12.19)
Gene namesi
Name:hcaE
Synonyms:digA, hcaA, hcaA1, phdC1, yfhU
Ordered Locus Names:b2538, JW2522
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13456. hcaE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850611 – 4533-phenylpropionate/cinnamic acid dioxygenase subunit alphaAdd BLAST453

Proteomic databases

PaxDbiP0ABR5.
PRIDEiP0ABR5.

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Protein-protein interaction databases

BioGridi4261577. 12 interactors.
IntActiP0ABR5. 5 interactors.
STRINGi511145.b2538.

Structurei

3D structure databases

ProteinModelPortaliP0ABR5.
SMRiP0ABR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 142RieskeAdd BLAST99

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Phylogenomic databases

eggNOGiENOG4105FUY. Bacteria.
COG4638. LUCA.
HOGENOMiHOG000105925.
InParanoidiP0ABR5.
KOiK05708.
OMAiKANGEHK.
PhylomeDBiP0ABR5.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
HAMAPiMF_01648. HcaE. 1 hit.
InterProiIPR020875. HcaE.
IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ABR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES
60 70 80 90 100
QIPKPGDFFN TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT
110 120 130 140 150
RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG
160 170 180 190 200
LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW
210 220 230 240 250
KFPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK
260 270 280 290 300
DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA
310 320 330 340 350
LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK
360 370 380 390 400
AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ
410 420 430 440 450
EKRRDDGIPG ITNYIFSETA ARGMYQRWAD LLSSESWQEV LDKTAAYQQE

VMK
Length:453
Mass (Da):51,109
Last modified:November 8, 2005 - v1
Checksum:i02535BF5F47643FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20V → A in CAA86018 (Ref. 1) Curated1
Sequence conflicti384 – 453GHRAR…QEVMK → ATAPATANCVWKWGLVRKSA ATTAFLALLTISFQKLPLVE CTNAGPIF in CAA86018 (Ref. 1) CuratedAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86018.1.
U00096 Genomic DNA. Translation: AAC75591.1.
AP009048 Genomic DNA. Translation: BAA16441.1.
PIRiA65031.
RefSeqiNP_417033.1. NC_000913.3.
WP_000211172.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75591; AAC75591; b2538.
BAA16441; BAA16441; BAA16441.
GeneIDi946998.
KEGGiecj:JW2522.
eco:b2538.
PATRICi32120473. VBIEscCol129921_2639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86018.1.
U00096 Genomic DNA. Translation: AAC75591.1.
AP009048 Genomic DNA. Translation: BAA16441.1.
PIRiA65031.
RefSeqiNP_417033.1. NC_000913.3.
WP_000211172.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABR5.
SMRiP0ABR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261577. 12 interactors.
IntActiP0ABR5. 5 interactors.
STRINGi511145.b2538.

Proteomic databases

PaxDbiP0ABR5.
PRIDEiP0ABR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75591; AAC75591; b2538.
BAA16441; BAA16441; BAA16441.
GeneIDi946998.
KEGGiecj:JW2522.
eco:b2538.
PATRICi32120473. VBIEscCol129921_2639.

Organism-specific databases

EchoBASEiEB3229.
EcoGeneiEG13456. hcaE.

Phylogenomic databases

eggNOGiENOG4105FUY. Bacteria.
COG4638. LUCA.
HOGENOMiHOG000105925.
InParanoidiP0ABR5.
KOiK05708.
OMAiKANGEHK.
PhylomeDBiP0ABR5.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:PHENYLPRODIOXY-MONOMER.
ECOL316407:JW2522-MONOMER.
MetaCyc:PHENYLPRODIOXY-MONOMER.

Miscellaneous databases

PROiP0ABR5.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
HAMAPiMF_01648. HcaE. 1 hit.
InterProiIPR020875. HcaE.
IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCAE_ECOLI
AccessioniPrimary (citable) accession number: P0ABR5
Secondary accession number(s): P77590, P78203, Q47139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.