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Protein

3-phenylpropionate/cinnamic acid dioxygenase subunit alpha

Gene

hcaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.1 Publication

Catalytic activityi

3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+.
(2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi87Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1
Metal bindingi105Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi108Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1
Metal bindingi213IronBy similarity1
Metal bindingi218IronBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENYLPRODIOXY-MONOMER
MetaCyc:PHENYLPRODIOXY-MONOMER
UniPathwayiUPA00714

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (EC:1.14.12.19)
Gene namesi
Name:hcaE
Synonyms:digA, hcaA, hcaA1, phdC1, yfhU
Ordered Locus Names:b2538, JW2522
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13456 hcaE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850611 – 4533-phenylpropionate/cinnamic acid dioxygenase subunit alphaAdd BLAST453

Proteomic databases

PaxDbiP0ABR5
PRIDEiP0ABR5

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Protein-protein interaction databases

BioGridi4261577, 13 interactors
IntActiP0ABR5, 5 interactors
STRINGi316385.ECDH10B_2705

Structurei

3D structure databases

ProteinModelPortaliP0ABR5
SMRiP0ABR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 142RieskeAdd BLAST99

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105FUY Bacteria
COG4638 LUCA
HOGENOMiHOG000105925
InParanoidiP0ABR5
KOiK05708
OMAiGEYCEDF
PhylomeDBiP0ABR5

Family and domain databases

Gene3Di2.102.10.10, 1 hit
HAMAPiMF_01648 HcaE, 1 hit
InterProiView protein in InterPro
IPR020875 HcaE
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR015881 Ring-hydroxy_dOase_2Fe2S_BS
IPR015879 Ring_hydroxy_dOase_asu_C_dom
IPR001663 Rng_hydr_dOase-A
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF00848 Ring_hydroxyl_A, 1 hit
PRINTSiPR00090 RNGDIOXGNASE
SUPFAMiSSF50022 SSF50022, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit
PS00570 RING_HYDROXYL_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES
60 70 80 90 100
QIPKPGDFFN TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT
110 120 130 140 150
RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG
160 170 180 190 200
LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW
210 220 230 240 250
KFPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK
260 270 280 290 300
DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA
310 320 330 340 350
LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK
360 370 380 390 400
AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ
410 420 430 440 450
EKRRDDGIPG ITNYIFSETA ARGMYQRWAD LLSSESWQEV LDKTAAYQQE

VMK
Length:453
Mass (Da):51,109
Last modified:November 8, 2005 - v1
Checksum:i02535BF5F47643FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20V → A in CAA86018 (Ref. 1) Curated1
Sequence conflicti384 – 453GHRAR…QEVMK → ATAPATANCVWKWGLVRKSA ATTAFLALLTISFQKLPLVE CTNAGPIF in CAA86018 (Ref. 1) CuratedAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA Translation: CAA86018.1
U00096 Genomic DNA Translation: AAC75591.1
AP009048 Genomic DNA Translation: BAA16441.1
PIRiA65031
RefSeqiNP_417033.1, NC_000913.3
WP_000211172.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75591; AAC75591; b2538
BAA16441; BAA16441; BAA16441
GeneIDi946998
KEGGiecj:JW2522
eco:b2538
PATRICifig|1411691.4.peg.4196

Similar proteinsi

Entry informationi

Entry nameiHCAE_ECOLI
AccessioniPrimary (citable) accession number: P0ABR5
Secondary accession number(s): P77590, P78203, Q47139
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health