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P0ABQ5

- DYR_ECOL6

UniProt

P0ABQ5 - DYR_ECOL6

Protein

Dihydrofolate reductase

Gene

folA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei5 – 51Substrate; via carbonyl oxygenBy similarity
    Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei27 – 271SubstrateBy similarity
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei76 – 761NADP; via carbonyl oxygenBy similarity
    Binding sitei113 – 1131SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 197NADPBy similarity
    Nucleotide bindingi45 – 462NADPBy similarity
    Nucleotide bindingi63 – 642NADPBy similarity
    Nucleotide bindingi95 – 1028NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:folA
    Ordered Locus Names:c0058
    OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
    Taxonomic identifieri199310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001410: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159Dihydrofolate reductasePRO_0000186388Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1239592.
    STRINGi199310.c0058.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ABQ5.
    SMRiP0ABQ5. Positions 1-159.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 158158DHFRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000040233.
    KOiK00287.
    OMAiCITHVEA.
    OrthoDBiEOG6KT2V2.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000194. DHFR. 1 hit.
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ABQ5-1 [UniParc]FASTAAdd to Basket

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    MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV IMGRHTWESI    50
    GRPLPGRKNI ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY 100
    EQFLPKAQKL YLTHIDAEVE GDTHFPDYEP DDWESVFSEF HDADAQNSHS 150
    YCFEILERR 159
    Length:159
    Mass (Da):17,999
    Last modified:July 21, 1986 - v1
    Checksum:i6A03CDCD7F5F8562
    GO

    Sequence cautioni

    The sequence AAN78554.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN78554.1. Different initiation.
    RefSeqiNP_752010.1. NC_004431.1.

    Genome annotation databases

    EnsemblBacteriaiAAN78554; AAN78554; c0058.
    GeneIDi1035973.
    KEGGiecc:c0058.
    PATRICi18278199. VBIEscCol75197_0055.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN78554.1 . Different initiation.
    RefSeqi NP_752010.1. NC_004431.1.

    3D structure databases

    ProteinModelPortali P0ABQ5.
    SMRi P0ABQ5. Positions 1-159.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1239592.
    STRINGi 199310.c0058.

    Chemistry

    BindingDBi P0ABQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN78554 ; AAN78554 ; c0058 .
    GeneIDi 1035973.
    KEGGi ecc:c0058.
    PATRICi 18278199. VBIEscCol75197_0055.

    Phylogenomic databases

    HOGENOMi HOG000040233.
    KOi K00287.
    OMAi CITHVEA.
    OrthoDBi EOG6KT2V2.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000194. DHFR. 1 hit.
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFT073 / ATCC 700928 / UPEC.

    Entry informationi

    Entry nameiDYR_ECOL6
    AccessioniPrimary (citable) accession number: P0ABQ5
    Secondary accession number(s): P00379
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3