Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0ABQ5 (DYR_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Ordered Locus Names:c0058
OrganismEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier199310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence caution

The sequence AAN78554.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Dihydrofolate reductase
PRO_0000186388

Regions

Domain1 – 158158DHFR
Nucleotide binding13 – 197NADP By similarity
Nucleotide binding45 – 462NADP By similarity
Nucleotide binding63 – 642NADP By similarity
Nucleotide binding95 – 1028NADP By similarity

Sites

Binding site51Substrate; via carbonyl oxygen By similarity
Binding site71NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site271Substrate By similarity
Binding site521Substrate By similarity
Binding site571Substrate By similarity
Binding site761NADP; via carbonyl oxygen By similarity
Binding site1131Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0ABQ5 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 6A03CDCD7F5F8562

FASTA15917,999
        10         20         30         40         50         60 
MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV IMGRHTWESI GRPLPGRKNI 

        70         80         90        100        110        120 
ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE 

       130        140        150 
GDTHFPDYEP DDWESVFSEF HDADAQNSHS YCFEILERR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN78554.1. Different initiation.
RefSeqNP_752010.1. NC_004431.1.

3D structure databases

ProteinModelPortalP0ABQ5.
SMRP0ABQ5. Positions 1-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1239592.
STRING199310.c0058.

Chemistry

BindingDBP0ABQ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN78554; AAN78554; c0058.
GeneID1035973.
KEGGecc:c0058.
PATRIC18278199. VBIEscCol75197_0055.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000040233.
KOK00287.
OMACITHVEA.
OrthoDBEOG6KT2V2.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_ECOL6
AccessionPrimary (citable) accession number: P0ABQ5
Secondary accession number(s): P00379
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways