##gff-version 3
P0ABQ4	UniProtKB	Chain	1	159	.	.	.	ID=PRO_0000186387;Note=Dihydrofolate reductase	
P0ABQ4	UniProtKB	Domain	1	158	.	.	.	Note=DHFR;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00660	
P0ABQ4	UniProtKB	Binding site	5	5	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:9012674;Dbxref=PMID:9012674	
P0ABQ4	UniProtKB	Binding site	7	7	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	13	19	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	27	27	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:9012674;Dbxref=PMID:9012674	
P0ABQ4	UniProtKB	Binding site	45	46	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	52	52	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:9012674;Dbxref=PMID:9012674	
P0ABQ4	UniProtKB	Binding site	57	57	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:9012674;Dbxref=PMID:9012674	
P0ABQ4	UniProtKB	Binding site	63	64	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	76	76	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	95	102	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19374017;Dbxref=PMID:19374017	
P0ABQ4	UniProtKB	Binding site	113	113	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:9012674;Dbxref=PMID:9012674	
P0ABQ4	UniProtKB	Natural variant	28	28	.	.	.	Note=In strain: B[RT500] isozyme 2. L->R	
P0ABQ4	UniProtKB	Natural variant	30	30	.	.	.	Note=In strain: 1810. W->G	
P0ABQ4	UniProtKB	Natural variant	154	154	.	.	.	Note=In strain: B[MB1428]. E->K	
P0ABQ4	UniProtKB	Natural variant	154	154	.	.	.	Note=In strain: 1810. E->Q	
P0ABQ4	UniProtKB	Mutagenesis	16	16	.	.	.	Note=Increases catalytic rate about 2-fold. M->F%2CS;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	16	16	.	.	.	Note=Increases catalytic rate about 2-fold. Increases catalytic rate about 7-fold%3B when associated with L-20%3B Y-42%3B F-92%3B A-85 and S-152. M->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	20	20	.	.	.	Note=Increases catalytic rate 2-fold. M->I%2CV;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	20	20	.	.	.	Note=Increases catalytic rate 2.5-fold. Increases catalytic rate about 7-fold%3B when associated with N-16%3B Y-42%3B F-92%3B A-85 and S-152. M->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	42	42	.	.	.	Note=Increases catalytic rate almost 2-fold. M->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	42	42	.	.	.	Note=Increases catalytic rate almost 2-fold. Increases catalytic rate about 7-fold%3B when associated with N-16%3B L-20%3B A-85%3B F-92 and S-152. M->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	85	85	.	.	.	Note=Decreases catalytic rate by one third. Increases catalytic rate about 7-fold%3B when associated with N-16%3B L-20%3B Y-42%3B F-92 and S-152. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	92	92	.	.	.	Note=No effect. Increases catalytic rate about 7-fold%3B when associated with N-16%3B L-20%3B Y-42%3B A-85 and S-152. M->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	92	92	.	.	.	Note=No effect. M->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Mutagenesis	152	152	.	.	.	Note=Increases catalytic rate 1.5-fold. Increases catalytic rate about 7-fold%3B when associated with N-16%3B L-20%3B Y-42%3B A-85 and F-92. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16510443;Dbxref=PMID:16510443	
P0ABQ4	UniProtKB	Beta strand	2	8	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Helix	10	12	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	13	16	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:8DAI	
P0ABQ4	UniProtKB	Helix	17	19	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Turn	20	23	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5UIH	
P0ABQ4	UniProtKB	Helix	25	35	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	40	43	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Helix	44	50	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	55	57	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4X5J	
P0ABQ4	UniProtKB	Beta strand	59	62	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	72	77	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Helix	78	85	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	91	93	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Helix	97	103	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Helix	104	106	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	107	115	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	122	124	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:8DAI	
P0ABQ4	UniProtKB	Helix	130	132	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	133	141	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7	
P0ABQ4	UniProtKB	Beta strand	147	149	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4KJJ	
P0ABQ4	UniProtKB	Beta strand	151	158	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6CW7