Reviewed, UniProtKB/Swiss-Prot P0ABQ3 (GARR_ECOL6)

Names and origin

Protein names

Recommended name:
    2-hydroxy-3-oxopropionate reductase
    EC=1.1.1.60
Alternative name(s):
    Tartronate semialdehyde reductase
      Short name=TSAR

Gene names

Name:	garR
Ordered Locus Names:	c3880

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-glycerate + NAD(P)+ = 2-hydroxy-3-oxopropanoate + NAD(P)H.
Pathway	Carbohydrate acid metabolism; D-galactarate degradation; D-glycerate from D-galactarate: step 3/3.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-hydroxy-3-oxopropionate reductase activity Inferred from electronic annotation. Source: EC 3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro coenzyme binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	2-hydroxy-3-oxopropionate reductase		PRO_0000173060
Regions
	Nucleotide binding	4 – 18	15	NAD By similarity
Sites
	Active site	170	1	By similarity
	Binding site	95	1	NAD By similarity
	Binding site	238	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABQ3-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 17DA392C2253278C	FASTA	294	30,427
	10 20 30 40 50 60 MKVGFIGLGI MGKPMSKNLL KAGYSLVVAD RNPEAIADVI AAGAETASTA KAIAEQCDVI 70 80 90 100 110 120 ITMLPNSPHV KEVALGENGI IEGAKPGTVL IDMSSIAPLA SREISEALKA KGIDMLDAPV 130 140 150 160 170 180 SGGEPKAIDG TLSVMVGGDK AIFDKYYDLM KAMAGSVVHT GEIGAGNVTK LANQVIVALN 190 200 210 220 230 240 IAAMSEALTL ATKAGVNPDL VYQAIRGGLA GSTVLDAKAP MVMDRNFKPG FRIDLHIKDL 250 260 270 280 290 ANALDTSHGV GAQLPLTAAV MEMMQALRAD GLGTADHSAL ACYYEKLAKV EVTR

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN82321.1. Different initiation.
RefSeq	NP_755747.2.
3D structure databases
SMR	P0ABQ3. Positions 1-294.
ModBase	Search...
Genome annotation databases
GeneID	1040122.
GenomeReviews	Gene locus c3880 in contig AE014075_GR.
KEGG	ecc:c3880.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729179.
OMA	WSRRRES.
Enzyme and pathway databases
BRENDA	1.1.1.60. 292881.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR008927. 6-PGluconate_DH_C-like. IPR006183. 6-phosphogluconate_DH. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006398. Tartro_sem_red. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00076. 6PGDHDRGNASE.
TIGRFAMs	TIGR01505. tartro_sem_red. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GARR_ECOL6

Accession

Primary (citable) accession number: P0ABQ3
Secondary accession number(s): P23523

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)