ID   GARR_ECOLI              Reviewed;         294 AA.
AC   P0ABQ2; P23523; Q2M984;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=2-hydroxy-3-oxopropionate reductase;
DE            EC=1.1.1.60;
DE   AltName: Full=Tartronate semialdehyde reductase;
DE            Short=TSAR;
GN   Name=garR; Synonyms=yhaE; OrderedLocusNames=b3125, JW5526;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   MEDLINE=91154140; PubMed=1705543;
RA   Komine Y., Inokuchi H.;
RT   "Precise mapping of the rnpB gene encoding the RNA component of RNase
RT   P in Escherichia coli K-12.";
RL   J. Bacteriol. 173:1813-1816(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   CHARACTERIZATION.
RX   MEDLINE=98447507; PubMed=9772162; DOI=10.1021/bi981124f;
RA   Hubbard B.K., Koch M., Palmer D.R., Babbitt P.C., Gerlt J.A.;
RT   "Evolution of enzymatic activities in the enolase superfamily:
RT   characterization of the (D)-glucarate/galactarate catabolic pathway in
RT   Escherichia coli.";
RL   Biochemistry 37:14369-14375(1998).
RN   [5]
RP   GENE NAME.
RX   MEDLINE=20225875; PubMed=10762278;
RX   DOI=10.1128/JB.182.9.2672-2674.2000;
RA   Monterrubio R., Baldoma L., Obradors N., Aguilar J., Badia J.;
RT   "A common regulator for the operons encoding the enzymes involved in
RT   D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia
RT   coli.";
RL   J. Bacteriol. 182:2672-2674(2000).
CC   -!- CATALYTIC ACTIVITY: (R)-glycerate + NAD(P)(+) = 2-hydroxy-3-
CC       oxopropanoate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate acid metabolism; D-galactaric acid
CC       degradation; D-glyceric acid from D-galactaric acid: step 3/3.
CC   -!- SIMILARITY: Belongs to the 3-hydroxyisobutyrate dehydrogenase
CC       family.
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DR   EMBL; D90212; BAA14238.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U18997; AAA57928.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC76159.3; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77172.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; AP_003671.1; -.
DR   RefSeq; NP_417594.3; -.
DR   SMR; P0ABQ2; 1-294.
DR   GeneID; 947631; -.
DR   GenomeReviews; AP009048_GR; JW5526.
DR   GenomeReviews; U00096_GR; b3125.
DR   KEGG; ecj:JW5526; -.
DR   KEGG; eco:b3125; -.
DR   EchoBASE; EB1163; -.
DR   EcoGene; EG11176; garR.
DR   HOGENOM; P0ABQ2; -.
DR   OMA; P0ABQ2; HTGAIGA.
DR   BioCyc; EcoCyc:TSA-REDUCT-MON; -.
DR   BioCyc; MetaCyc:TSA-REDUCT-MON; -.
DR   GO; GO:0008679; F:2-hydroxy-3-oxopropionate reductase activity; IEA:EC.
DR   GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004616; F:phosphogluconate dehydrogenase (decarboxyla...; IEA:InterPro.
DR   GO; GO:0046487; P:glyoxylate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
DR   GO; GO:0006573; P:valine metabolic process; IEA:InterPro.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR015815; 3hydroxyacid_DH/Rdtase.
DR   InterPro; IPR006183; 6-phosphogluconate_DH.
DR   InterPro; IPR006115; 6PGDH_NAD-bd.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006398; Tartro_sem_red.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR22981; 3hydroxy_acid_DH; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PRINTS; PR00076; 6PGDHDRGNASE.
DR   TIGRFAMs; TIGR01505; tartro_sem_red; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; NAD; Oxidoreductase.
FT   CHAIN         1    294       2-hydroxy-3-oxopropionate reductase.
FT                                /FTId=PRO_0000173059.
FT   ACT_SITE    170    170       By similarity.
SQ   SEQUENCE   294 AA;  30427 MW;  17DA392C2253278C CRC64;
     MKVGFIGLGI MGKPMSKNLL KAGYSLVVAD RNPEAIADVI AAGAETASTA KAIAEQCDVI
     ITMLPNSPHV KEVALGENGI IEGAKPGTVL IDMSSIAPLA SREISEALKA KGIDMLDAPV
     SGGEPKAIDG TLSVMVGGDK AIFDKYYDLM KAMAGSVVHT GEIGAGNVTK LANQVIVALN
     IAAMSEALTL ATKAGVNPDL VYQAIRGGLA GSTVLDAKAP MVMDRNFKPG FRIDLHIKDL
     ANALDTSHGV GAQLPLTAAV MEMMQALRAD GLGTADHSAL ACYYEKLAKV EVTR
//