Reviewed, UniProtKB/Swiss-Prot P0ABQ2 (GARR_ECOLI)

Names and origin

Protein names

Recommended name:
    2-hydroxy-3-oxopropionate reductase
    EC=1.1.1.60
Alternative name(s):
    Tartronate semialdehyde reductase
      Short name=TSAR

Gene names

Name:	garR
Synonyms:	yhaE
Ordered Locus Names:	b3125, JW5526

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-glycerate + NAD(P)+ = 2-hydroxy-3-oxopropanoate + NAD(P)H.
Pathway	Carbohydrate acid metabolism; D-galactaric acid degradation; D-glyceric acid from D-galactaric acid: step 3/3.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-hydroxy-3-oxopropionate reductase activity Inferred from electronic annotation. Source: EC 3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro coenzyme binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	2-hydroxy-3-oxopropionate reductase		PRO_0000173059
Sites
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABQ2-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 17DA392C2253278C	FASTA	294	30,427
	10 20 30 40 50 60 MKVGFIGLGI MGKPMSKNLL KAGYSLVVAD RNPEAIADVI AAGAETASTA KAIAEQCDVI 70 80 90 100 110 120 ITMLPNSPHV KEVALGENGI IEGAKPGTVL IDMSSIAPLA SREISEALKA KGIDMLDAPV 130 140 150 160 170 180 SGGEPKAIDG TLSVMVGGDK AIFDKYYDLM KAMAGSVVHT GEIGAGNVTK LANQVIVALN 190 200 210 220 230 240 IAAMSEALTL ATKAGVNPDL VYQAIRGGLA GSTVLDAKAP MVMDRNFKPG FRIDLHIKDL 250 260 270 280 290 ANALDTSHGV GAQLPLTAAV MEMMQALRAD GLGTADHSAL ACYYEKLAKV EVTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Precise mapping of the rnpB gene encoding the RNA component of RNase P in Escherichia coli K-12." Komine Y., Inokuchi H. J. Bacteriol. 173:1813-1816(1991) [PubMed: 1705543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Hubbard B.K., Koch M., Palmer D.R., Babbitt P.C., Gerlt J.A. Biochemistry 37:14369-14375(1998) [PubMed: 9772162] [Abstract] Cited for: CHARACTERIZATION.
[5]	"A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." Monterrubio R., Baldoma L., Obradors N., Aguilar J., Badia J. J. Bacteriol. 182:2672-2674(2000) [PubMed: 10762278] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90212 Genomic DNA. Translation: BAA14238.1. Different initiation. U18997 Genomic DNA. Translation: AAA57928.1. Different initiation. U00096 Genomic DNA. Translation: AAC76159.3. AP009048 Genomic DNA. Translation: BAE77172.1. Different initiation.
RefSeq	AP_003671.1. NP_417594.3.
3D structure databases
SMR	P0ABQ2. Positions 1-294.
ModBase	Search...
Genome annotation databases
GeneID	947631.
GenomeReviews	Gene locus JW5526 in contig AP009048_GR. Gene locus b3125 in contig U00096_GR.
KEGG	ecj:JW5526. eco:b3125.
Organism-specific databases
EchoBASE	EB1163.
EcoGene	EG11176. garR.
CMR	Search...
Phylogenomic databases
HOGENOM	P0ABQ2.
OMA	P0ABQ2. HTGAIGA.
Enzyme and pathway databases
BioCyc	EcoCyc:TSA-REDUCT-MON. MetaCyc:TSA-REDUCT-MON.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR006183. 6-phosphogluconate_DH. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006398. Tartro_sem_red. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00076. 6PGDHDRGNASE.
TIGRFAMs	TIGR01505. tartro_sem_red. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GARR_ECOLI

Accession

Primary (citable) accession number: P0ABQ2
Secondary accession number(s): P23523, Q2M984

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)