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P0ABP9

- DEOD_ECO57

UniProt

P0ABP9 - DEOD_ECO57

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Protein
Purine nucleoside phosphorylase DeoD-type
Gene
deoD, Z5986, ECs5343
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules By similarity.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.UniRule annotation

GO - Molecular functioni

  1. purine-nucleoside phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. purine nucleoside metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL386585:GJFA-5355-MONOMER.
ECOO157:DEOD-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase DeoD-type (EC:2.4.2.1)
Short name:
PNP
Gene namesi
Name:deoD
Ordered Locus Names:Z5986, ECs5343
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 239238Purine nucleoside phosphorylase DeoD-typeUniRule annotation
PRO_0000063131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP0ABP9.

Interactioni

Subunit structurei

Homohexamer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z5986.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195
Helixi24 – 3310
Beta strandi35 – 417
Helixi43 – 453
Beta strandi48 – 536
Beta strandi56 – 616
Helixi67 – 8014
Beta strandi85 – 939
Beta strandi104 – 11310
Helixi116 – 1205
Turni121 – 1233
Helixi132 – 14413
Beta strandi149 – 1568
Helixi166 – 1738
Beta strandi178 – 1825
Helixi183 – 19210
Beta strandi196 – 20510
Helixi215 – 23723

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PK7X-ray2.50A/B/C2-238[»]
1PK9X-ray1.90A/B/C2-238[»]
1PKEX-ray2.30A/B/C2-238[»]
1PR0X-ray2.20A/B/C1-239[»]
1PR1X-ray2.30A/B/C1-239[»]
1PR2X-ray2.30A/B/C1-239[»]
1PR4X-ray2.40A/B/C1-239[»]
1PR5X-ray2.50A/B/C1-239[»]
1PR6X-ray2.10A/B/C1-239[»]
1PW7X-ray2.00A/B/C1-239[»]
ProteinModelPortaliP0ABP9.
SMRiP0ABP9. Positions 2-238.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0813.
HOGENOMiHOG000274896.
KOiK03784.
OMAiRQTTFSE.
OrthoDBiEOG6BKJC5.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01627. Pur_nucleosid_phosp.
InterProiIPR004402. DeoD-type.
IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR00107. deoD. 1 hit.
PROSITEiPS01232. PNP_UDP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABP9-1 [UniParc]FASTAAdd to Basket

« Hide

MATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT    50
GTYKGRKISV MGHGMGIPSC SIYTKELITD FGVKKIIRVG SCGAVLPHVK 100
LRDVVIGMGA CTDSKVNRIR FKDHDFAAIA DFDMVRNAVD AAKALGIDAR 150
VGNLFSADLF YSPDGEMFDV MEKYGILGVE MEAAGIYGVA AEFGAKALTI 200
CTVSDHIRTH EQTTAAERQT TFNDMIKIAL ESVLLGDKE 239
Length:239
Mass (Da):25,950
Last modified:January 23, 2007 - v2
Checksum:i71D3DFAA5A176970
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG59565.1.
BA000007 Genomic DNA. Translation: BAB38766.1.
PIRiA86138.
G91296.
RefSeqiNP_290998.1. NC_002655.2.
NP_313370.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG59565; AAG59565; Z5986.
BAB38766; BAB38766; BAB38766.
GeneIDi913500.
959670.
KEGGiece:Z5986.
ecs:ECs5343.
PATRICi18360344. VBIEscCol44059_5302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG59565.1 .
BA000007 Genomic DNA. Translation: BAB38766.1 .
PIRi A86138.
G91296.
RefSeqi NP_290998.1. NC_002655.2.
NP_313370.1. NC_002695.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PK7 X-ray 2.50 A/B/C 2-238 [» ]
1PK9 X-ray 1.90 A/B/C 2-238 [» ]
1PKE X-ray 2.30 A/B/C 2-238 [» ]
1PR0 X-ray 2.20 A/B/C 1-239 [» ]
1PR1 X-ray 2.30 A/B/C 1-239 [» ]
1PR2 X-ray 2.30 A/B/C 1-239 [» ]
1PR4 X-ray 2.40 A/B/C 1-239 [» ]
1PR5 X-ray 2.50 A/B/C 1-239 [» ]
1PR6 X-ray 2.10 A/B/C 1-239 [» ]
1PW7 X-ray 2.00 A/B/C 1-239 [» ]
ProteinModelPortali P0ABP9.
SMRi P0ABP9. Positions 2-238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z5986.

Proteomic databases

PRIDEi P0ABP9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG59565 ; AAG59565 ; Z5986 .
BAB38766 ; BAB38766 ; BAB38766 .
GeneIDi 913500.
959670.
KEGGi ece:Z5986.
ecs:ECs5343.
PATRICi 18360344. VBIEscCol44059_5302.

Phylogenomic databases

eggNOGi COG0813.
HOGENOMi HOG000274896.
KOi K03784.
OMAi RQTTFSE.
OrthoDBi EOG6BKJC5.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-5355-MONOMER.
ECOO157:DEOD-MONOMER.

Miscellaneous databases

PROi P0ABP9.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01627. Pur_nucleosid_phosp.
InterProi IPR004402. DeoD-type.
IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view ]
PANTHERi PTHR21234. PTHR21234. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR00107. deoD. 1 hit.
PROSITEi PS01232. PNP_UDP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
  3. "Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase."
    Bennett E.M., Li C., Allan P.W., Parker W.B., Ealick S.E.
    J. Biol. Chem. 278:47110-47118(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH PURINE NUCLEOSIDES.

Entry informationi

Entry nameiDEOD_ECO57
AccessioniPrimary (citable) accession number: P0ABP9
Secondary accession number(s): P09743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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