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P0ABP9

- DEOD_ECO57

UniProt

P0ABP9 - DEOD_ECO57

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Protein

Purine nucleoside phosphorylase DeoD-type

Gene

deoD

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.By similarity

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.UniRule annotation

GO - Molecular functioni

  1. purine-nucleoside phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. purine nucleoside metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL386585:GJFA-5355-MONOMER.
ECOO157:DEOD-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase DeoD-typeUniRule annotation (EC:2.4.2.1UniRule annotation)
Short name:
PNPUniRule annotation
Gene namesi
Name:deoDUniRule annotation
Ordered Locus Names:Z5986, ECs5343
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 239238Purine nucleoside phosphorylase DeoD-typePRO_0000063131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271N6-acetyllysineUniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP0ABP9.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z5986.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Helixi24 – 3310Combined sources
Beta strandi35 – 417Combined sources
Helixi43 – 453Combined sources
Beta strandi48 – 536Combined sources
Beta strandi56 – 616Combined sources
Helixi67 – 8014Combined sources
Beta strandi85 – 939Combined sources
Beta strandi104 – 11310Combined sources
Helixi116 – 1205Combined sources
Turni121 – 1233Combined sources
Helixi132 – 14413Combined sources
Beta strandi149 – 1568Combined sources
Helixi166 – 1738Combined sources
Beta strandi178 – 1825Combined sources
Helixi183 – 19210Combined sources
Beta strandi196 – 20510Combined sources
Helixi215 – 23723Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PK7X-ray2.50A/B/C2-238[»]
1PK9X-ray1.90A/B/C2-238[»]
1PKEX-ray2.30A/B/C2-238[»]
1PR0X-ray2.20A/B/C1-239[»]
1PR1X-ray2.30A/B/C1-239[»]
1PR2X-ray2.30A/B/C1-239[»]
1PR4X-ray2.40A/B/C1-239[»]
1PR5X-ray2.50A/B/C1-239[»]
1PR6X-ray2.10A/B/C1-239[»]
1PW7X-ray2.00A/B/C1-239[»]
ProteinModelPortaliP0ABP9.
SMRiP0ABP9. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0813.
HOGENOMiHOG000274896.
KOiK03784.
OMAiRQTTFSE.
OrthoDBiEOG6BKJC5.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01627. Pur_nucleosid_phosp.
InterProiIPR004402. DeoD-type.
IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR00107. deoD. 1 hit.
PROSITEiPS01232. PNP_UDP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABP9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT
60 70 80 90 100
GTYKGRKISV MGHGMGIPSC SIYTKELITD FGVKKIIRVG SCGAVLPHVK
110 120 130 140 150
LRDVVIGMGA CTDSKVNRIR FKDHDFAAIA DFDMVRNAVD AAKALGIDAR
160 170 180 190 200
VGNLFSADLF YSPDGEMFDV MEKYGILGVE MEAAGIYGVA AEFGAKALTI
210 220 230
CTVSDHIRTH EQTTAAERQT TFNDMIKIAL ESVLLGDKE
Length:239
Mass (Da):25,950
Last modified:January 23, 2007 - v2
Checksum:i71D3DFAA5A176970
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59565.1.
BA000007 Genomic DNA. Translation: BAB38766.1.
PIRiA86138.
G91296.
RefSeqiNP_290998.1. NC_002655.2.
NP_313370.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG59565; AAG59565; Z5986.
BAB38766; BAB38766; BAB38766.
GeneIDi913500.
959670.
KEGGiece:Z5986.
ecs:ECs5343.
PATRICi18360344. VBIEscCol44059_5302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59565.1 .
BA000007 Genomic DNA. Translation: BAB38766.1 .
PIRi A86138.
G91296.
RefSeqi NP_290998.1. NC_002655.2.
NP_313370.1. NC_002695.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PK7 X-ray 2.50 A/B/C 2-238 [» ]
1PK9 X-ray 1.90 A/B/C 2-238 [» ]
1PKE X-ray 2.30 A/B/C 2-238 [» ]
1PR0 X-ray 2.20 A/B/C 1-239 [» ]
1PR1 X-ray 2.30 A/B/C 1-239 [» ]
1PR2 X-ray 2.30 A/B/C 1-239 [» ]
1PR4 X-ray 2.40 A/B/C 1-239 [» ]
1PR5 X-ray 2.50 A/B/C 1-239 [» ]
1PR6 X-ray 2.10 A/B/C 1-239 [» ]
1PW7 X-ray 2.00 A/B/C 1-239 [» ]
ProteinModelPortali P0ABP9.
SMRi P0ABP9. Positions 2-238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z5986.

Proteomic databases

PRIDEi P0ABP9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG59565 ; AAG59565 ; Z5986 .
BAB38766 ; BAB38766 ; BAB38766 .
GeneIDi 913500.
959670.
KEGGi ece:Z5986.
ecs:ECs5343.
PATRICi 18360344. VBIEscCol44059_5302.

Phylogenomic databases

eggNOGi COG0813.
HOGENOMi HOG000274896.
KOi K03784.
OMAi RQTTFSE.
OrthoDBi EOG6BKJC5.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-5355-MONOMER.
ECOO157:DEOD-MONOMER.

Miscellaneous databases

PROi P0ABP9.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01627. Pur_nucleosid_phosp.
InterProi IPR004402. DeoD-type.
IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view ]
PANTHERi PTHR21234. PTHR21234. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR00107. deoD. 1 hit.
PROSITEi PS01232. PNP_UDP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
  3. "Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase."
    Bennett E.M., Li C., Allan P.W., Parker W.B., Ealick S.E.
    J. Biol. Chem. 278:47110-47118(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH PURINE NUCLEOSIDES.

Entry informationi

Entry nameiDEOD_ECO57
AccessioniPrimary (citable) accession number: P0ABP9
Secondary accession number(s): P09743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3