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P0ABP9 (DEOD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purine nucleoside phosphorylase DeoD-type

Short name=PNP
EC=2.4.2.1
Gene names
Name:deoD
Ordered Locus Names:Z5986, ECs5343
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules By similarity. HAMAP-Rule MF_01627

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01627

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01627

Subunit structure

Homohexamer By similarity. HAMAP-Rule MF_01627

Sequence similarities

Belongs to the PNP/UDP phosphorylase family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processpurine nucleoside metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionpurine-nucleoside phosphorylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 239238Purine nucleoside phosphorylase DeoD-type HAMAP-Rule MF_01627
PRO_0000063131

Amino acid modifications

Modified residue271N6-acetyllysine By similarity

Secondary structure

................................... 239
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ABP9 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 71D3DFAA5A176970

FASTA23925,950
        10         20         30         40         50         60 
MATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT GTYKGRKISV 

        70         80         90        100        110        120 
MGHGMGIPSC SIYTKELITD FGVKKIIRVG SCGAVLPHVK LRDVVIGMGA CTDSKVNRIR 

       130        140        150        160        170        180 
FKDHDFAAIA DFDMVRNAVD AAKALGIDAR VGNLFSADLF YSPDGEMFDV MEKYGILGVE 

       190        200        210        220        230 
MEAAGIYGVA AEFGAKALTI CTVSDHIRTH EQTTAAERQT TFNDMIKIAL ESVLLGDKE 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
[3]"Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase."
Bennett E.M., Li C., Allan P.W., Parker W.B., Ealick S.E.
J. Biol. Chem. 278:47110-47118(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH PURINE NUCLEOSIDES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG59565.1.
BA000007 Genomic DNA. Translation: BAB38766.1.
PIRA86138.
G91296.
RefSeqNP_290998.1. NC_002655.2.
NP_313370.1. NC_002695.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PK7X-ray2.50A/B/C2-238[»]
1PK9X-ray1.90A/B/C2-238[»]
1PKEX-ray2.30A/B/C2-238[»]
1PR0X-ray2.20A/B/C1-239[»]
1PR1X-ray2.30A/B/C1-239[»]
1PR2X-ray2.30A/B/C1-239[»]
1PR4X-ray2.40A/B/C1-239[»]
1PR5X-ray2.50A/B/C1-239[»]
1PR6X-ray2.10A/B/C1-239[»]
1PW7X-ray2.00A/B/C1-239[»]
ProteinModelPortalP0ABP9.
SMRP0ABP9. Positions 2-238.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z5986.

Proteomic databases

PRIDEP0ABP9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG59565; AAG59565; Z5986.
BAB38766; BAB38766; BAB38766.
GeneID913500.
959670.
KEGGece:Z5986.
ecs:ECs5343.
PATRIC18360344. VBIEscCol44059_5302.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0813.
HOGENOMHOG000274896.
KOK03784.
OMARQTTFSE.
OrthoDBEOG6BKJC5.

Enzyme and pathway databases

BioCycECOL386585:GJFA-5355-MONOMER.
ECOO157:DEOD-MONOMER.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01627. Pur_nucleosid_phosp.
InterProIPR004402. DeoD-type.
IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERPTHR21234. PTHR21234. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR00107. deoD. 1 hit.
PROSITEPS01232. PNP_UDP_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0ABP9.

Entry information

Entry nameDEOD_ECO57
AccessionPrimary (citable) accession number: P0ABP9
Secondary accession number(s): P09743
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references