P0ABP9 (DEOD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine nucleoside phosphorylase deoD-type
Short name=PNP
EC=2.4.2.1

Gene names

Name:	deoD
Ordered Locus Names:	Z5986, ECs5343

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules By similarity. HAMAP MF_01627
Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP MF_01627
Subunit structure	Homohexamer By similarity. HAMAP MF_01627
Sequence similarities	Belongs to the PNP/UDP phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	purine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 239	238	Purine nucleoside phosphorylase deoD-type HAMAP MF_01627		PRO_0000063131
Amino acid modifications
	Modified residue	27	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABP9 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 71D3DFAA5A176970	FASTA	239	25,950
	10 20 30 40 50 60 MATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT GTYKGRKISV 70 80 90 100 110 120 MGHGMGIPSC SIYTKELITD FGVKKIIRVG SCGAVLPHVK LRDVVIGMGA CTDSKVNRIR 130 140 150 160 170 180 FKDHDFAAIA DFDMVRNAVD AAKALGIDAR VGNLFSADLF YSPDGEMFDV MEKYGILGVE 190 200 210 220 230 MEAAGIYGVA AEFGAKALTI CTVSDHIRTH EQTTAAERQT TFNDMIKIAL ESVLLGDKE

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG59565.1. BA000007 Genomic DNA. Translation: BAB38766.1.
PIR	A86138. G91296.
RefSeq	NP_290998.1. NC_002655.2. NP_313370.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0ABP9.
SMR	P0ABP9. Positions 2-238.
ModBase	Search...
Proteomic databases
PRIDE	P0ABP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026262; EBESCP00000025155; EBESCG00000025315. EBESCT00000056540; EBESCP00000054368; EBESCG00000055588.
GeneID	913500. 959670.
GenomeReviews	Gene locus Z5986 in contig AE005174_GR. Gene locus ECs5343 in contig BA000007_GR.
KEGG	ece:Z5986. ecs:ECs5343.
PATRIC	18360344. VBIEscCol44059_5302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009056.
HOGENOM	HBG617197.
OMA	SFETHAF.
ProtClustDB	PRK05819.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS5343-MONOMER.
Family and domain databases
HAMAP	MF_01627. Pur_nucleosid_phosp. [Tree]
InterPro	IPR004402. DeoD. IPR018017. Nucleoside_phosphorylase. IPR018016. Nucleoside_phosphorylase_CS. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K03784.
PANTHER	PTHR21234. PNP_UDP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00107. DeoD. 1 hit.
PROSITE	PS01232. PNP_UDP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEOD_ECO57

Accession

Primary (citable) accession number: P0ABP9
Secondary accession number(s): P09743

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program