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Protein

Anaerobic C4-dicarboxylate transporter DcuA

Gene

dcuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane.

GO - Molecular functioni

GO - Biological processi

  • C4-dicarboxylate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:DCUA-MONOMER.
ECOL316407:JW5735-MONOMER.
MetaCyc:DCUA-MONOMER.

Protein family/group databases

TCDBi2.A.13.1.1. the c4-dicarboxylate uptake (dcu) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic C4-dicarboxylate transporter DcuA
Gene namesi
Name:dcuA
Synonyms:genA
Ordered Locus Names:b4138, JW5735
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11225. dcuA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 1818HelicalAdd
BLAST
Topological domaini19 – 191Cytoplasmic
Transmembranei20 – 3718HelicalAdd
BLAST
Topological domaini38 – 5316PeriplasmicAdd
BLAST
Transmembranei54 – 7118HelicalAdd
BLAST
Topological domaini72 – 8514CytoplasmicAdd
BLAST
Transmembranei86 – 10318HelicalAdd
BLAST
Topological domaini104 – 13229PeriplasmicAdd
BLAST
Transmembranei133 – 14715HelicalAdd
BLAST
Topological domaini148 – 22881CytoplasmicAdd
BLAST
Transmembranei229 – 24618HelicalAdd
BLAST
Topological domaini247 – 26418PeriplasmicAdd
BLAST
Transmembranei265 – 28218HelicalAdd
BLAST
Topological domaini283 – 29210Cytoplasmic
Transmembranei293 – 31018HelicalAdd
BLAST
Topological domaini311 – 33222PeriplasmicAdd
BLAST
Transmembranei333 – 35018HelicalAdd
BLAST
Topological domaini351 – 3555Cytoplasmic
Transmembranei356 – 37318HelicalAdd
BLAST
Topological domaini374 – 43360PeriplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Anaerobic C4-dicarboxylate transporter DcuAPRO_0000170347Add
BLAST

Proteomic databases

EPDiP0ABN5.
PaxDbiP0ABN5.
PRIDEiP0ABN5.

Interactioni

Protein-protein interaction databases

BioGridi4261707. 7 interactions.
STRINGi511145.b4138.

Structurei

3D structure databases

ProteinModelPortaliP0ABN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DS2. Bacteria.
COG2704. LUCA.
HOGENOMiHOG000272417.
KOiK07791.
OMAiPKQINYL.
OrthoDBiEOG6FNHP5.
PhylomeDBiP0ABN5.

Family and domain databases

InterProiIPR004668. Anaer_Dcu_memb_transpt.
[Graphical view]
PfamiPF03605. DcuA_DcuB. 1 hit.
[Graphical view]
PIRSFiPIRSF004539. C4-dicrbxl_trns. 1 hit.
TIGRFAMsiTIGR00770. Dcu. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVVELIIVL LAIFLGARLG GIGIGFAGGL GVLVLAAIGV KPGNIPFDVI
60 70 80 90 100
SIIMAVIAAI SAMQVAGGLD YLVHQTEKLL RRNPKYITIL APIVTYFLTI
110 120 130 140 150
FAGTGNISLA TLPVIAEVAK EQGVKPCRPL STAVVSAQIA ITASPISAAV
160 170 180 190 200
VYMSSVMEGH GISYLHLLSV VIPSTLLAVL VMSFLVTMLF NSKLSDDPIY
210 220 230 240 250
RKRLEEGLVE LRGEKQIEIK SGAKTSVWLF LLGVVGVVIY AIINSPSMGL
260 270 280 290 300
VEKPLMNTTN AILIIMLSVA TLTTVICKVD TDNILNSSTF KAGMSACICI
310 320 330 340 350
LGVAWLGDTF VSNNIDWIKD TAGEVIQGHP WLLAVIFFFA SALLYSQAAT
360 370 380 390 400
AKALMPMALA LNVSPLTAVA SFAAVSGLFI LPTYPTLVAA VQMDDTGTTR
410 420 430
IGKFVFNHPF FIPGTLGVAL AVCFGFVLGS FML
Length:433
Mass (Da):45,751
Last modified:November 8, 2005 - v1
Checksum:i049C8D186C83C9AD
GO

Sequence cautioni

The sequence BAE78140.1 differs from that shown. Reason: Frameshift at position 60. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti355 – 3562MP → IA in CAA26176 (PubMed:2987841).Curated
Sequence conflicti365 – 3673PLT → RXD (PubMed:2987841).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79887 Genomic DNA. Translation: CAA56259.1.
U14003 Genomic DNA. Translation: AAA97037.1.
U00096 Genomic DNA. Translation: AAC77098.1.
AP009048 Genomic DNA. Translation: BAE78140.1. Frameshift.
X02307 Genomic DNA. Translation: CAA26176.1. Sequence problems.
X04066 Genomic DNA. Translation: CAA27702.1.
PIRiS56366. QQEC94.
RefSeqiNP_418561.1. NC_000913.3.
WP_000961959.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77098; AAC77098; b4138.
BAE78140; BAE78140; BAE78140.
GeneIDi948659.
KEGGiecj:JW5735.
eco:b4138.
PATRICi32123843. VBIEscCol129921_4269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79887 Genomic DNA. Translation: CAA56259.1.
U14003 Genomic DNA. Translation: AAA97037.1.
U00096 Genomic DNA. Translation: AAC77098.1.
AP009048 Genomic DNA. Translation: BAE78140.1. Frameshift.
X02307 Genomic DNA. Translation: CAA26176.1. Sequence problems.
X04066 Genomic DNA. Translation: CAA27702.1.
PIRiS56366. QQEC94.
RefSeqiNP_418561.1. NC_000913.3.
WP_000961959.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261707. 7 interactions.
STRINGi511145.b4138.

Protein family/group databases

TCDBi2.A.13.1.1. the c4-dicarboxylate uptake (dcu) family.

Proteomic databases

EPDiP0ABN5.
PaxDbiP0ABN5.
PRIDEiP0ABN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77098; AAC77098; b4138.
BAE78140; BAE78140; BAE78140.
GeneIDi948659.
KEGGiecj:JW5735.
eco:b4138.
PATRICi32123843. VBIEscCol129921_4269.

Organism-specific databases

EchoBASEiEB1207.
EcoGeneiEG11225. dcuA.

Phylogenomic databases

eggNOGiENOG4105DS2. Bacteria.
COG2704. LUCA.
HOGENOMiHOG000272417.
KOiK07791.
OMAiPKQINYL.
OrthoDBiEOG6FNHP5.
PhylomeDBiP0ABN5.

Enzyme and pathway databases

BioCyciEcoCyc:DCUA-MONOMER.
ECOL316407:JW5735-MONOMER.
MetaCyc:DCUA-MONOMER.

Miscellaneous databases

PROiP0ABN5.

Family and domain databases

InterProiIPR004668. Anaer_Dcu_memb_transpt.
[Graphical view]
PfamiPF03605. DcuA_DcuB. 1 hit.
[Graphical view]
PIRSFiPIRSF004539. C4-dicrbxl_trns. 1 hit.
TIGRFAMsiTIGR00770. Dcu. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction and properties of Escherichia coli mutants defective in two genes encoding homologous membrane proteins with putative roles in anaerobic C4-dicarboxylic acid transport."
    Six S., Andrews S.C., Roberts R.E., Unden G., Guest J.R.
    Biochem. Soc. Trans. 21:342S-342S(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct)."
    Six S., Andrews S.C., Unden G., Guest J.R.
    J. Bacteriol. 176:6470-6478(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W."
    Takagi J.S., Ida N., Tokushige M., Sakamoto H., Shimura Y.
    Nucleic Acids Res. 13:2063-2074(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-412.
    Strain: W / ATCC 11105 / DSM 1900.
  7. "Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12."
    Woods S.A., Miles J.S., Roberts R.E., Guest J.R.
    Biochem. J. 237:547-557(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-264.
    Strain: K12.
  8. "Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12."
    Bell P.J., Andrews S.C., Sivak M.N., Guest J.R.
    J. Bacteriol. 171:3494-3503(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  9. "Topological analysis of DcuA, an anaerobic C4-dicarboxylate transporter of Escherichia coli."
    Golby P., Kelly D.J., Guest J.R., Andrews S.C.
    J. Bacteriol. 180:4821-4827(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  10. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiDCUA_ECOLI
AccessioniPrimary (citable) accession number: P0ABN5
Secondary accession number(s): P04539, Q2M6G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 8, 2005
Last modified: March 16, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.