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Protein

Anaerobic C4-dicarboxylate transporter DcuA

Gene

dcuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane.

GO - Molecular functioni

GO - Biological processi

  • C4-dicarboxylate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:DCUA-MONOMER.
ECOL316407:JW5735-MONOMER.
MetaCyc:DCUA-MONOMER.

Protein family/group databases

TCDBi2.A.13.1.1. the c4-dicarboxylate uptake (dcu) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic C4-dicarboxylate transporter DcuA
Gene namesi
Name:dcuA
Synonyms:genA
Ordered Locus Names:b4138, JW5735
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11225. dcuA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 18HelicalAdd BLAST18
Topological domaini19Cytoplasmic1
Transmembranei20 – 37HelicalAdd BLAST18
Topological domaini38 – 53PeriplasmicAdd BLAST16
Transmembranei54 – 71HelicalAdd BLAST18
Topological domaini72 – 85CytoplasmicAdd BLAST14
Transmembranei86 – 103HelicalAdd BLAST18
Topological domaini104 – 132PeriplasmicAdd BLAST29
Transmembranei133 – 147HelicalAdd BLAST15
Topological domaini148 – 228CytoplasmicAdd BLAST81
Transmembranei229 – 246HelicalAdd BLAST18
Topological domaini247 – 264PeriplasmicAdd BLAST18
Transmembranei265 – 282HelicalAdd BLAST18
Topological domaini283 – 292Cytoplasmic10
Transmembranei293 – 310HelicalAdd BLAST18
Topological domaini311 – 332PeriplasmicAdd BLAST22
Transmembranei333 – 350HelicalAdd BLAST18
Topological domaini351 – 355Cytoplasmic5
Transmembranei356 – 373HelicalAdd BLAST18
Topological domaini374 – 433PeriplasmicAdd BLAST60

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001703471 – 433Anaerobic C4-dicarboxylate transporter DcuAAdd BLAST433

Proteomic databases

PaxDbiP0ABN5.
PRIDEiP0ABN5.

Interactioni

Protein-protein interaction databases

BioGridi4261707. 7 interactors.
STRINGi511145.b4138.

Structurei

3D structure databases

ProteinModelPortaliP0ABN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DS2. Bacteria.
COG2704. LUCA.
HOGENOMiHOG000272417.
KOiK07791.
OMAiPKQINYL.
PhylomeDBiP0ABN5.

Family and domain databases

InterProiIPR004668. Anaer_Dcu_memb_transpt.
[Graphical view]
PfamiPF03605. DcuA_DcuB. 1 hit.
[Graphical view]
PIRSFiPIRSF004539. C4-dicrbxl_trns. 1 hit.
TIGRFAMsiTIGR00770. Dcu. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVVELIIVL LAIFLGARLG GIGIGFAGGL GVLVLAAIGV KPGNIPFDVI
60 70 80 90 100
SIIMAVIAAI SAMQVAGGLD YLVHQTEKLL RRNPKYITIL APIVTYFLTI
110 120 130 140 150
FAGTGNISLA TLPVIAEVAK EQGVKPCRPL STAVVSAQIA ITASPISAAV
160 170 180 190 200
VYMSSVMEGH GISYLHLLSV VIPSTLLAVL VMSFLVTMLF NSKLSDDPIY
210 220 230 240 250
RKRLEEGLVE LRGEKQIEIK SGAKTSVWLF LLGVVGVVIY AIINSPSMGL
260 270 280 290 300
VEKPLMNTTN AILIIMLSVA TLTTVICKVD TDNILNSSTF KAGMSACICI
310 320 330 340 350
LGVAWLGDTF VSNNIDWIKD TAGEVIQGHP WLLAVIFFFA SALLYSQAAT
360 370 380 390 400
AKALMPMALA LNVSPLTAVA SFAAVSGLFI LPTYPTLVAA VQMDDTGTTR
410 420 430
IGKFVFNHPF FIPGTLGVAL AVCFGFVLGS FML
Length:433
Mass (Da):45,751
Last modified:November 8, 2005 - v1
Checksum:i049C8D186C83C9AD
GO

Sequence cautioni

The sequence BAE78140 differs from that shown. Reason: Frameshift at position 60.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti355 – 356MP → IA in CAA26176 (PubMed:2987841).Curated2
Sequence conflicti365 – 367PLT → RXD (PubMed:2987841).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79887 Genomic DNA. Translation: CAA56259.1.
U14003 Genomic DNA. Translation: AAA97037.1.
U00096 Genomic DNA. Translation: AAC77098.1.
AP009048 Genomic DNA. Translation: BAE78140.1. Frameshift.
X02307 Genomic DNA. Translation: CAA26176.1. Sequence problems.
X04066 Genomic DNA. Translation: CAA27702.1.
PIRiS56366. QQEC94.
RefSeqiNP_418561.1. NC_000913.3.
WP_000961959.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77098; AAC77098; b4138.
BAE78140; BAE78140; BAE78140.
GeneIDi948659.
KEGGiecj:JW5735.
eco:b4138.
PATRICi32123843. VBIEscCol129921_4269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79887 Genomic DNA. Translation: CAA56259.1.
U14003 Genomic DNA. Translation: AAA97037.1.
U00096 Genomic DNA. Translation: AAC77098.1.
AP009048 Genomic DNA. Translation: BAE78140.1. Frameshift.
X02307 Genomic DNA. Translation: CAA26176.1. Sequence problems.
X04066 Genomic DNA. Translation: CAA27702.1.
PIRiS56366. QQEC94.
RefSeqiNP_418561.1. NC_000913.3.
WP_000961959.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261707. 7 interactors.
STRINGi511145.b4138.

Protein family/group databases

TCDBi2.A.13.1.1. the c4-dicarboxylate uptake (dcu) family.

Proteomic databases

PaxDbiP0ABN5.
PRIDEiP0ABN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77098; AAC77098; b4138.
BAE78140; BAE78140; BAE78140.
GeneIDi948659.
KEGGiecj:JW5735.
eco:b4138.
PATRICi32123843. VBIEscCol129921_4269.

Organism-specific databases

EchoBASEiEB1207.
EcoGeneiEG11225. dcuA.

Phylogenomic databases

eggNOGiENOG4105DS2. Bacteria.
COG2704. LUCA.
HOGENOMiHOG000272417.
KOiK07791.
OMAiPKQINYL.
PhylomeDBiP0ABN5.

Enzyme and pathway databases

BioCyciEcoCyc:DCUA-MONOMER.
ECOL316407:JW5735-MONOMER.
MetaCyc:DCUA-MONOMER.

Miscellaneous databases

PROiP0ABN5.

Family and domain databases

InterProiIPR004668. Anaer_Dcu_memb_transpt.
[Graphical view]
PfamiPF03605. DcuA_DcuB. 1 hit.
[Graphical view]
PIRSFiPIRSF004539. C4-dicrbxl_trns. 1 hit.
TIGRFAMsiTIGR00770. Dcu. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCUA_ECOLI
AccessioniPrimary (citable) accession number: P0ABN5
Secondary accession number(s): P04539, Q2M6G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.