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Protein

Diacylglycerol kinase

Gene

dgkA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Recycling of diacylglycerol produced during the turnover of membrane phospholipid.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-5028-MONOMER.
ECOO157:DGKA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAGK
Alternative name(s):
Diglyceride kinase
Short name:
DGK
Gene namesi
Name:dgkA
Ordered Locus Names:Z5641, ECs5025
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3332CytoplasmicBy similarityAdd
BLAST
Transmembranei34 – 4916HelicalBy similarityAdd
BLAST
Topological domaini50 – 523PeriplasmicBy similarity
Transmembranei53 – 6917HelicalBy similarityAdd
BLAST
Topological domaini70 – 9526CytoplasmicBy similarityAdd
BLAST
Transmembranei96 – 11924HelicalBy similarityAdd
BLAST
Topological domaini120 – 1223PeriplasmicBy similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 122121Diacylglycerol kinasePRO_0000195261Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5641.

Structurei

3D structure databases

ProteinModelPortaliP0ABN3.
SMRiP0ABN3. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0818.
HOGENOMiHOG000014819.
KOiK00901.
OMAiIACFLDV.
OrthoDBiEOG60W7XM.

Family and domain databases

InterProiIPR000829. Diacylglycerol_kinase_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD
60 70 80 90 100
VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA
110 120
AVLIAIIVAV ITWCILLWSH FG
Length:122
Mass (Da):13,245
Last modified:January 23, 2007 - v2
Checksum:iD5B4F86925FA6A17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59241.1.
BA000007 Genomic DNA. Translation: BAB38448.1.
PIRiA91257.
E86097.
RefSeqiNP_290676.1. NC_002655.2.
NP_313052.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG59241; AAG59241; Z5641.
BAB38448; BAB38448; BAB38448.
GeneIDi914310.
960081.
KEGGiece:Z5641.
ecs:ECs5025.
PATRICi18359645. VBIEscCol44059_4959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59241.1.
BA000007 Genomic DNA. Translation: BAB38448.1.
PIRiA91257.
E86097.
RefSeqiNP_290676.1. NC_002655.2.
NP_313052.1. NC_002695.1.

3D structure databases

ProteinModelPortaliP0ABN3.
SMRiP0ABN3. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59241; AAG59241; Z5641.
BAB38448; BAB38448; BAB38448.
GeneIDi914310.
960081.
KEGGiece:Z5641.
ecs:ECs5025.
PATRICi18359645. VBIEscCol44059_4959.

Phylogenomic databases

eggNOGiCOG0818.
HOGENOMiHOG000014819.
KOiK00901.
OMAiIACFLDV.
OrthoDBiEOG60W7XM.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-5028-MONOMER.
ECOO157:DGKA-MONOMER.

Family and domain databases

InterProiIPR000829. Diacylglycerol_kinase_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiKDGL_ECO57
AccessioniPrimary (citable) accession number: P0ABN3
Secondary accession number(s): P00556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.