P0ABN3 (KDGL_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase
Short name=DAGK
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase
Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	Z5641, ECs5025

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 122	121	Diacylglycerol kinase		PRO_0000195261
Regions
	Topological domain	2 – 33	32	Cytoplasmic By similarity
	Transmembrane	34 – 49	16	Helical; By similarity
	Topological domain	50 – 52	3	Periplasmic By similarity
	Transmembrane	53 – 69	17	Helical; By similarity
	Topological domain	70 – 95	26	Cytoplasmic By similarity
	Transmembrane	96 – 119	24	Helical; By similarity
	Topological domain	120 – 122	3	Periplasmic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABN3 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D5B4F86925FA6A17	FASTA	122	13,245
	10 20 30 40 50 60 MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI 70 80 90 100 110 120 SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG59241.1. BA000007 Genomic DNA. Translation: BAB38448.1.
PIR	A91257. E86097.
RefSeq	NP_290676.1. NC_002655.2. NP_313052.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0ABN3.
SMR	P0ABN3. Positions 2-122.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z5641.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG59241; AAG59241; Z5641. BAB38448; BAB38448; BAB38448.
GeneID	914310. 960081.
KEGG	ece:Z5641. ecs:ECs5025.
PATRIC	18359645. VBIEscCol44059_4959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0818.
HOGENOM	HOG000014819.
KO	K00901.
OMA	ITNEWHE.
ProtClustDB	CLSK879806.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-5028-MONOMER.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGL_ECO57

Accession

Primary (citable) accession number: P0ABN3
Secondary accession number(s): P00556

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program