Reviewed, UniProtKB/Swiss-Prot P0ABN2 (KDGL_ECOL6)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase
      Short name=DAGK
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase
      Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	c5013

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 122	121	Diacylglycerol kinase		PRO_0000195262
Regions
	Topological domain	2 – 33	32	Cytoplasmic By similarity
	Transmembrane	34 – 49	16	By similarity
	Topological domain	50 – 52	3	Periplasmic By similarity
	Transmembrane	53 – 69	17	By similarity
	Topological domain	70 – 95	26	Cytoplasmic By similarity
	Transmembrane	96 – 119	24	By similarity
	Topological domain	120 – 122	3	Periplasmic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABN2-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D5B4F86925FA6A17	FASTA	122	13,245
	10 20 30 40 50 60 MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI 70 80 90 100 110 120 SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN83439.1.
RefSeq	NP_756865.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1039398.
GenomeReviews	Gene locus c5013 in contig AE014075_GR.
KEGG	ecc:c5013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0ABN2.
OMA	ITNEWHE.
Enzyme and pathway databases
BRENDA	2.7.1.107. 292881.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
ProDom	PD010722. DAGK_prokar. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGL_ECOL6

Accession

Primary (citable) accession number: P0ABN2
Secondary accession number(s): P00556

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)