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P0ABN1

- KDGL_ECOLI

UniProt

P0ABN1 - KDGL_ECOLI

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Protein
Diacylglycerol kinase
Gene
dgkA, b4042, JW4002
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recycling of diacylglycerol produced during the turnover of membrane phospholipid.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. phosphatidic acid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAGK
Alternative name(s):
Diglyceride kinase
Short name:
DGK
Gene namesi
Name:dgkA
Ordered Locus Names:b4042, JW4002
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10224. dgkA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3332Cytoplasmic Inferred
Add
BLAST
Transmembranei34 – 4916Helical; Inferred
Add
BLAST
Topological domaini50 – 523Periplasmic Inferred
Transmembranei53 – 6917Helical; Inferred
Add
BLAST
Topological domaini70 – 9526Cytoplasmic Inferred
Add
BLAST
Transmembranei96 – 11924Helical; Inferred
Add
BLAST
Topological domaini120 – 1223Periplasmic Inferred

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: EcoCyc
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 122121Diacylglycerol kinase
PRO_0000195260Add
BLAST

Proteomic databases

PaxDbiP0ABN1.
PRIDEiP0ABN1.

Expressioni

Gene expression databases

GenevestigatoriP0ABN1.

Interactioni

Protein-protein interaction databases

BioGridi852837. 2 interactions.
DIPiDIP-60228N.
STRINGi511145.b4042.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2821
Helixi30 – 4819
Helixi53 – 8331
Helixi89 – 12032

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KDCNMR-A/B/C2-122[»]
3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
3ZE4X-ray3.70A/B/C2-122[»]
3ZE5X-ray3.10A/B/C2-122[»]
4BPDX-ray3.30A/B/C/D/E/F2-122[»]
4BRBX-ray2.55A/B/C/D/E/F2-122[»]
4BRRX-ray2.44A/B/C/D/E/F2-122[»]
ProteinModelPortaliP0ABN1.

Miscellaneous databases

EvolutionaryTraceiP0ABN1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0818.
HOGENOMiHOG000014819.
KOiK00901.
OMAiFKNTSYA.
OrthoDBiEOG60W7XM.
PhylomeDBiP0ABN1.

Family and domain databases

InterProiIPR000829. Diacylglycerol_kinase_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABN1-1 [UniParc]FASTAAdd to Basket

« Hide

MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD    50
VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA 100
AVLIAIIVAV ITWCILLWSH FG 122
Length:122
Mass (Da):13,245
Last modified:January 23, 2007 - v2
Checksum:iD5B4F86925FA6A17
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.
PIRiA00667. KIECDG.
RefSeqiNP_418466.1. NC_000913.3.
YP_492185.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.
GeneIDi12932725.
948543.
KEGGiecj:Y75_p3929.
eco:b4042.
PATRICi32123621. VBIEscCol129921_4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1 .
U00006 Genomic DNA. Translation: AAC43136.1 .
U00096 Genomic DNA. Translation: AAC77012.1 .
AP009048 Genomic DNA. Translation: BAE78044.1 .
PIRi A00667. KIECDG.
RefSeqi NP_418466.1. NC_000913.3.
YP_492185.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KDC NMR - A/B/C 2-122 [» ]
3ZE3 X-ray 2.05 A/B/C/D/E/F 2-122 [» ]
3ZE4 X-ray 3.70 A/B/C 2-122 [» ]
3ZE5 X-ray 3.10 A/B/C 2-122 [» ]
4BPD X-ray 3.30 A/B/C/D/E/F 2-122 [» ]
4BRB X-ray 2.55 A/B/C/D/E/F 2-122 [» ]
4BRR X-ray 2.44 A/B/C/D/E/F 2-122 [» ]
ProteinModelPortali P0ABN1.
ModBasei Search...

Protein-protein interaction databases

BioGridi 852837. 2 interactions.
DIPi DIP-60228N.
STRINGi 511145.b4042.

Proteomic databases

PaxDbi P0ABN1.
PRIDEi P0ABN1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77012 ; AAC77012 ; b4042 .
BAE78044 ; BAE78044 ; BAE78044 .
GeneIDi 12932725.
948543.
KEGGi ecj:Y75_p3929.
eco:b4042.
PATRICi 32123621. VBIEscCol129921_4159.

Organism-specific databases

EchoBASEi EB0220.
EcoGenei EG10224. dgkA.

Phylogenomic databases

eggNOGi COG0818.
HOGENOMi HOG000014819.
KOi K00901.
OMAi FKNTSYA.
OrthoDBi EOG60W7XM.
PhylomeDBi P0ABN1.

Enzyme and pathway databases

BioCyci EcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ABN1.
PROi P0ABN1.

Gene expression databases

Genevestigatori P0ABN1.

Family and domain databases

InterProi IPR000829. Diacylglycerol_kinase_prok.
[Graphical view ]
Pfami PF01219. DAGK_prokar. 1 hit.
[Graphical view ]
PROSITEi PS01069. DAGK_PROKAR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequences encoding plsB and dgk loci of Escherichia coli."
    Lightner V.A., Bell R.M., Modrich P.
    J. Biol. Chem. 258:10856-10861(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis."
    Loomis C.R., Walsh J.P., Bell R.M.
    J. Biol. Chem. 260:4091-4097(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 2-3 AND 121.
  6. "Membrane topology of Escherichia coli diacylglycerol kinase."
    Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II
    J. Bacteriol. 176:5459-5465(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiKDGL_ECOLI
AccessioniPrimary (citable) accession number: P0ABN1
Secondary accession number(s): P00556, Q2M6R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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