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P0ABN1

- KDGL_ECOLI

UniProt

P0ABN1 - KDGL_ECOLI

Protein

Diacylglycerol kinase

Gene

dgkA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Recycling of diacylglycerol produced during the turnover of membrane phospholipid.

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: EcoCyc

    GO - Biological processi

    1. phosphatidic acid biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
    ECOL316407:JW4002-MONOMER.
    MetaCyc:DIACYLGLYKIN-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase (EC:2.7.1.107)
    Short name:
    DAGK
    Alternative name(s):
    Diglyceride kinase
    Short name:
    DGK
    Gene namesi
    Name:dgkA
    Ordered Locus Names:b4042, JW4002
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10224. dgkA.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: EcoCyc
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 122121Diacylglycerol kinasePRO_0000195260Add
    BLAST

    Proteomic databases

    PaxDbiP0ABN1.
    PRIDEiP0ABN1.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ABN1.

    Interactioni

    Protein-protein interaction databases

    BioGridi852837. 2 interactions.
    DIPiDIP-60228N.
    STRINGi511145.b4042.

    Structurei

    Secondary structure

    1
    122
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2821
    Helixi30 – 4819
    Helixi53 – 8331
    Helixi89 – 12032

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KDCNMR-A/B/C2-122[»]
    3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
    3ZE4X-ray3.70A/B/C2-122[»]
    3ZE5X-ray3.10A/B/C2-122[»]
    4BPDX-ray3.30A/B/C/D/E/F2-122[»]
    4BRBX-ray2.55A/B/C/D/E/F2-122[»]
    4BRRX-ray2.44A/B/C/D/E/F2-122[»]
    ProteinModelPortaliP0ABN1.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ABN1.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 3332CytoplasmicCuratedAdd
    BLAST
    Topological domaini50 – 523PeriplasmicCurated
    Topological domaini70 – 9526CytoplasmicCuratedAdd
    BLAST
    Topological domaini120 – 1223PeriplasmicCurated

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 4916HelicalCuratedAdd
    BLAST
    Transmembranei53 – 6917HelicalCuratedAdd
    BLAST
    Transmembranei96 – 11924HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0818.
    HOGENOMiHOG000014819.
    KOiK00901.
    OMAiFKNTSYA.
    OrthoDBiEOG60W7XM.
    PhylomeDBiP0ABN1.

    Family and domain databases

    InterProiIPR000829. Diacylglycerol_kinase_prok.
    [Graphical view]
    PfamiPF01219. DAGK_prokar. 1 hit.
    [Graphical view]
    PROSITEiPS01069. DAGK_PROKAR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0ABN1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD    50
    VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA 100
    AVLIAIIVAV ITWCILLWSH FG 122
    Length:122
    Mass (Da):13,245
    Last modified:January 23, 2007 - v2
    Checksum:iD5B4F86925FA6A17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00127 Genomic DNA. Translation: AAA24394.1.
    U00006 Genomic DNA. Translation: AAC43136.1.
    U00096 Genomic DNA. Translation: AAC77012.1.
    AP009048 Genomic DNA. Translation: BAE78044.1.
    PIRiA00667. KIECDG.
    RefSeqiNP_418466.1. NC_000913.3.
    YP_492185.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77012; AAC77012; b4042.
    BAE78044; BAE78044; BAE78044.
    GeneIDi12932725.
    948543.
    KEGGiecj:Y75_p3929.
    eco:b4042.
    PATRICi32123621. VBIEscCol129921_4159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00127 Genomic DNA. Translation: AAA24394.1 .
    U00006 Genomic DNA. Translation: AAC43136.1 .
    U00096 Genomic DNA. Translation: AAC77012.1 .
    AP009048 Genomic DNA. Translation: BAE78044.1 .
    PIRi A00667. KIECDG.
    RefSeqi NP_418466.1. NC_000913.3.
    YP_492185.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KDC NMR - A/B/C 2-122 [» ]
    3ZE3 X-ray 2.05 A/B/C/D/E/F 2-122 [» ]
    3ZE4 X-ray 3.70 A/B/C 2-122 [» ]
    3ZE5 X-ray 3.10 A/B/C 2-122 [» ]
    4BPD X-ray 3.30 A/B/C/D/E/F 2-122 [» ]
    4BRB X-ray 2.55 A/B/C/D/E/F 2-122 [» ]
    4BRR X-ray 2.44 A/B/C/D/E/F 2-122 [» ]
    ProteinModelPortali P0ABN1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 852837. 2 interactions.
    DIPi DIP-60228N.
    STRINGi 511145.b4042.

    Proteomic databases

    PaxDbi P0ABN1.
    PRIDEi P0ABN1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77012 ; AAC77012 ; b4042 .
    BAE78044 ; BAE78044 ; BAE78044 .
    GeneIDi 12932725.
    948543.
    KEGGi ecj:Y75_p3929.
    eco:b4042.
    PATRICi 32123621. VBIEscCol129921_4159.

    Organism-specific databases

    EchoBASEi EB0220.
    EcoGenei EG10224. dgkA.

    Phylogenomic databases

    eggNOGi COG0818.
    HOGENOMi HOG000014819.
    KOi K00901.
    OMAi FKNTSYA.
    OrthoDBi EOG60W7XM.
    PhylomeDBi P0ABN1.

    Enzyme and pathway databases

    BioCyci EcoCyc:DIACYLGLYKIN-MONOMER.
    ECOL316407:JW4002-MONOMER.
    MetaCyc:DIACYLGLYKIN-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0ABN1.
    PROi P0ABN1.

    Gene expression databases

    Genevestigatori P0ABN1.

    Family and domain databases

    InterProi IPR000829. Diacylglycerol_kinase_prok.
    [Graphical view ]
    Pfami PF01219. DAGK_prokar. 1 hit.
    [Graphical view ]
    PROSITEi PS01069. DAGK_PROKAR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequences encoding plsB and dgk loci of Escherichia coli."
      Lightner V.A., Bell R.M., Modrich P.
      J. Biol. Chem. 258:10856-10861(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
      Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
      Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis."
      Loomis C.R., Walsh J.P., Bell R.M.
      J. Biol. Chem. 260:4091-4097(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 2-3 AND 121.
    6. "Membrane topology of Escherichia coli diacylglycerol kinase."
      Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II
      J. Bacteriol. 176:5459-5465(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiKDGL_ECOLI
    AccessioniPrimary (citable) accession number: P0ABN1
    Secondary accession number(s): P00556, Q2M6R2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3