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Protein

Diacylglycerol kinase

Gene

dgkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recycling of diacylglycerol produced during the turnover of membrane phospholipid.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: EcoCyc

GO - Biological processi

  • phosphatidic acid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.
BRENDAi2.7.1.107. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAGK
Alternative name(s):
Diglyceride kinase
Short name:
DGK
Gene namesi
Name:dgkA
Ordered Locus Names:b4042, JW4002
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10224. dgkA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3332CytoplasmicCuratedAdd
BLAST
Transmembranei34 – 4916HelicalCuratedAdd
BLAST
Topological domaini50 – 523PeriplasmicCurated
Transmembranei53 – 6917HelicalCuratedAdd
BLAST
Topological domaini70 – 9526CytoplasmicCuratedAdd
BLAST
Transmembranei96 – 11924HelicalCuratedAdd
BLAST
Topological domaini120 – 1223PeriplasmicCurated

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 122121Diacylglycerol kinasePRO_0000195260Add
BLAST

Proteomic databases

PaxDbiP0ABN1.
PRIDEiP0ABN1.

Interactioni

Protein-protein interaction databases

BioGridi4261719. 272 interactions.
852837. 2 interactions.
DIPiDIP-60228N.
STRINGi511145.b4042.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2821Combined sources
Helixi30 – 4819Combined sources
Helixi53 – 8331Combined sources
Helixi89 – 12032Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KDCNMR-A/B/C2-122[»]
3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
3ZE4X-ray3.70A/B/C2-122[»]
3ZE5X-ray3.10A/B/C2-122[»]
4BPDX-ray3.30A/B/C/D/E/F2-122[»]
4BRBX-ray2.55A/B/C/D/E/F2-122[»]
4BRRX-ray2.44A/B/C/D/E/F2-122[»]
4CJZX-ray3.25A/B/C2-122[»]
4CK0X-ray2.92A/B/C2-122[»]
4D2EX-ray2.28A/B/C/D/E/F2-122[»]
4UP6X-ray3.80A/B/C2-122[»]
4UXWX-ray3.15A/B/C2-122[»]
4UXXX-ray2.70A/B/C2-122[»]
4UXZX-ray2.18A/B/C/D/E/F2-122[»]
4UYOX-ray2.18A/B/C/D/E/F2-122[»]
5D56X-ray2.80A/B/C/D/E/F2-122[»]
5D57X-ray2.80A/B/C/D/E/F2-122[»]
5DWKX-ray2.60A/B/C/D/E/F2-122[»]
ProteinModelPortaliP0ABN1.
SMRiP0ABN1. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABN1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41080IJ. Bacteria.
COG0818. LUCA.
HOGENOMiHOG000014819.
InParanoidiP0ABN1.
KOiK00901.
OMAiITNEWHE.
PhylomeDBiP0ABN1.

Family and domain databases

CDDicd14264. DAGK_IM. 1 hit.
InterProiIPR000829. DAGK.
IPR033718. DAGK_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD
60 70 80 90 100
VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA
110 120
AVLIAIIVAV ITWCILLWSH FG
Length:122
Mass (Da):13,245
Last modified:January 23, 2007 - v2
Checksum:iD5B4F86925FA6A17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.
PIRiA00667. KIECDG.
RefSeqiNP_418466.1. NC_000913.3.
WP_000002907.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.
GeneIDi948543.
KEGGiecj:JW4002.
eco:b4042.
PATRICi32123621. VBIEscCol129921_4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.
PIRiA00667. KIECDG.
RefSeqiNP_418466.1. NC_000913.3.
WP_000002907.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KDCNMR-A/B/C2-122[»]
3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
3ZE4X-ray3.70A/B/C2-122[»]
3ZE5X-ray3.10A/B/C2-122[»]
4BPDX-ray3.30A/B/C/D/E/F2-122[»]
4BRBX-ray2.55A/B/C/D/E/F2-122[»]
4BRRX-ray2.44A/B/C/D/E/F2-122[»]
4CJZX-ray3.25A/B/C2-122[»]
4CK0X-ray2.92A/B/C2-122[»]
4D2EX-ray2.28A/B/C/D/E/F2-122[»]
4UP6X-ray3.80A/B/C2-122[»]
4UXWX-ray3.15A/B/C2-122[»]
4UXXX-ray2.70A/B/C2-122[»]
4UXZX-ray2.18A/B/C/D/E/F2-122[»]
4UYOX-ray2.18A/B/C/D/E/F2-122[»]
5D56X-ray2.80A/B/C/D/E/F2-122[»]
5D57X-ray2.80A/B/C/D/E/F2-122[»]
5DWKX-ray2.60A/B/C/D/E/F2-122[»]
ProteinModelPortaliP0ABN1.
SMRiP0ABN1. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261719. 272 interactions.
852837. 2 interactions.
DIPiDIP-60228N.
STRINGi511145.b4042.

Proteomic databases

PaxDbiP0ABN1.
PRIDEiP0ABN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.
GeneIDi948543.
KEGGiecj:JW4002.
eco:b4042.
PATRICi32123621. VBIEscCol129921_4159.

Organism-specific databases

EchoBASEiEB0220.
EcoGeneiEG10224. dgkA.

Phylogenomic databases

eggNOGiENOG41080IJ. Bacteria.
COG0818. LUCA.
HOGENOMiHOG000014819.
InParanoidiP0ABN1.
KOiK00901.
OMAiITNEWHE.
PhylomeDBiP0ABN1.

Enzyme and pathway databases

BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.
BRENDAi2.7.1.107. 2026.

Miscellaneous databases

EvolutionaryTraceiP0ABN1.
PROiP0ABN1.

Family and domain databases

CDDicd14264. DAGK_IM. 1 hit.
InterProiIPR000829. DAGK.
IPR033718. DAGK_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKDGL_ECOLI
AccessioniPrimary (citable) accession number: P0ABN1
Secondary accession number(s): P00556, Q2M6R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.