Reviewed, UniProtKB/Swiss-Prot P0ABN1 (KDGL_ECOLI)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase
      Short name=DAGK
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase
      Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	b4042, JW4002

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 122	121	Diacylglycerol kinase		PRO_0000195260
Regions
	Topological domain	2 – 33	32	Cytoplasmic Probable
	Transmembrane	34 – 49	16	Probable
	Topological domain	50 – 52	3	Periplasmic Probable
	Transmembrane	53 – 69	17	Probable
	Topological domain	70 – 95	26	Cytoplasmic Probable
	Transmembrane	96 – 119	24	Probable
	Topological domain	120 – 122	3	Periplasmic Probable

Sequences

	Sequence		Length	Mass (Da)
	P0ABN1-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D5B4F86925FA6A17	FASTA	122	13,245
	10 20 30 40 50 60 MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI 70 80 90 100 110 120 SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequences encoding plsB and dgk loci of Escherichia coli." Lightner V.A., Bell R.M., Modrich P. J. Biol. Chem. 258:10856-10861(1983) [PubMed: 6309817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis." Loomis C.R., Walsh J.P., Bell R.M. J. Biol. Chem. 260:4091-4097(1985) [PubMed: 2984194] [Abstract] Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 2-3 AND 121.
[6]	"Membrane topology of Escherichia coli diacylglycerol kinase." Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II J. Bacteriol. 176:5459-5465(1994) [PubMed: 8071224] [Abstract] Cited for: TOPOLOGY.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.

PIR

KIECDG. A00667.

RefSeq

AP_004543.1.
NP_418466.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KDC	NMR	-	A/B/C	2-122	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0ABN1.

Genome annotation databases

GeneID

948543.

GenomeReviews

Gene locus JW4002 in contig AP009048_GR.
Gene locus b4042 in contig U00096_GR.

KEGG

ecj:JW4002.
eco:b4042.

Organism-specific databases

EchoBASE

EB0220.

EcoGene

EG10224. dgkA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0ABN1.

OMA

ITNEWHE.

Enzyme and pathway databases

BioCyc

EcoCyc:DIACYLGLYKIN-MON.
MetaCyc:DIACYLGLYKIN-MON.

Gene expression databases

Genevestigator

P0ABN1.

Family and domain databases

InterPro

IPR000829. Diacylglycerol_kinase_prok.
[Graphical view]

Pfam

PF01219. DAGK_prokar. 1 hit.
[Graphical view]

ProDom

PD010722. DAGK_prokar. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KDGL_ECOLI

Accession

Primary (citable) accession number: P0ABN1
Secondary accession number(s): P00556, Q2M6R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)