P0ABN1 (KDGL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase
Short name=DAGK
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase
Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	b4042, JW4002

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidic acid biosynthetic process Inferred from direct assay PubMed 2828054. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay Ref.5. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from direct assay PubMed 2828054. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 122

121

Diacylglycerol kinase

PRO_0000195260

Regions

Topological domain

2 – 33

32

Cytoplasmic Probable

Transmembrane

34 – 49

16

Helical; Probable

Topological domain

50 – 52

3

Periplasmic Probable

Transmembrane

53 – 69

17

Helical; Probable

Topological domain

70 – 95

26

Cytoplasmic Probable

Transmembrane

96 – 119

24

Helical; Probable

Topological domain

120 – 122

3

Periplasmic Probable

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

122

Helix Strand Turn

Details...

Helix

8 – 16

9

Helix

18 – 25

8

Turn

26 – 30

5

Helix

31 – 48

18

Helix

55 – 70

16

Helix

72 – 80

9

Helix

92 – 118

27

Sequences

	Sequence		Length	Mass (Da)
	P0ABN1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D5B4F86925FA6A17	FASTA	122	13,245
	10 20 30 40 50 60 MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI 70 80 90 100 110 120 SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequences encoding plsB and dgk loci of Escherichia coli." Lightner V.A., Bell R.M., Modrich P. J. Biol. Chem. 258:10856-10861(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis." Loomis C.R., Walsh J.P., Bell R.M. J. Biol. Chem. 260:4091-4097(1985) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 2-3 AND 121.
[6]	"Membrane topology of Escherichia coli diacylglycerol kinase." Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II J. Bacteriol. 176:5459-5465(1994) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.

PIR

KIECDG. A00667.

RefSeq

NP_418466.1. NC_000913.2.
YP_492185.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KDC	NMR	-	A/B/C	2-122	[»]

ProteinModelPortal

P0ABN1.

SMR

P0ABN1. Positions 2-122.

ModBase

Search...

Protein-protein interaction databases

STRING

511145.b4042.

Proteomic databases

PaxDb

P0ABN1.

PRIDE

P0ABN1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.

GeneID

12932725.
948543.

KEGG

ecj:Y75_p3929.
eco:b4042.

PATRIC

32123621. VBIEscCol129921_4159.

Organism-specific databases

EchoBASE

EB0220.

EcoGene

EG10224. dgkA.

Phylogenomic databases

eggNOG

COG0818.

HOGENOM

HOG000014819.

KO

K00901.

OMA

ITNEWHE.

ProtClustDB

CLSK879806.

Enzyme and pathway databases

BioCyc

EcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.

Gene expression databases

Genevestigator

P0ABN1.

Family and domain databases

InterPro

IPR000829. Diacylglycerol_kinase_prok.
[Graphical view]

Pfam

PF01219. DAGK_prokar. 1 hit.
[Graphical view]

PROSITE

PS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ABN1.

Entry information

Entry name

KDGL_ECOLI

Accession

Primary (citable) accession number: P0ABN1
Secondary accession number(s): P00556, Q2M6R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program