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Protein

Diacylglycerol kinase

Gene

dgkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recycling of diacylglycerol produced during the turnover of membrane phospholipid.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: EcoCyc

GO - Biological processi

  1. phosphatidic acid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.
BRENDAi2.7.1.107. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAGK
Alternative name(s):
Diglyceride kinase
Short name:
DGK
Gene namesi
Name:dgkA
Ordered Locus Names:b4042, JW4002
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10224. dgkA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3332CytoplasmicCuratedAdd
BLAST
Transmembranei34 – 4916HelicalCuratedAdd
BLAST
Topological domaini50 – 523PeriplasmicCurated
Transmembranei53 – 6917HelicalCuratedAdd
BLAST
Topological domaini70 – 9526CytoplasmicCuratedAdd
BLAST
Transmembranei96 – 11924HelicalCuratedAdd
BLAST
Topological domaini120 – 1223PeriplasmicCurated

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: EcoCyc
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 122121Diacylglycerol kinasePRO_0000195260Add
BLAST

Proteomic databases

PaxDbiP0ABN1.
PRIDEiP0ABN1.

Expressioni

Gene expression databases

GenevestigatoriP0ABN1.

Interactioni

Protein-protein interaction databases

BioGridi852837. 2 interactions.
DIPiDIP-60228N.
STRINGi511145.b4042.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2821Combined sources
Helixi30 – 4819Combined sources
Helixi53 – 8331Combined sources
Helixi89 – 12032Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KDCNMR-A/B/C2-122[»]
3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
3ZE4X-ray3.70A/B/C2-122[»]
3ZE5X-ray3.10A/B/C2-122[»]
4BPDX-ray3.30A/B/C/D/E/F2-122[»]
4BRBX-ray2.55A/B/C/D/E/F2-122[»]
4BRRX-ray2.44A/B/C/D/E/F2-122[»]
4CJZX-ray3.25A/B/C2-122[»]
4CK0X-ray2.92A/B/C2-122[»]
4D2EX-ray2.28A/B/C/D/E/F2-122[»]
4UP6X-ray3.80A/B/C2-122[»]
ProteinModelPortaliP0ABN1.
SMRiP0ABN1. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABN1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0818.
HOGENOMiHOG000014819.
InParanoidiP0ABN1.
KOiK00901.
OMAiIACFLDV.
OrthoDBiEOG60W7XM.
PhylomeDBiP0ABN1.

Family and domain databases

InterProiIPR000829. Diacylglycerol_kinase_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD
60 70 80 90 100
VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA
110 120
AVLIAIIVAV ITWCILLWSH FG
Length:122
Mass (Da):13,245
Last modified:January 23, 2007 - v2
Checksum:iD5B4F86925FA6A17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.
PIRiA00667. KIECDG.
RefSeqiNP_418466.1. NC_000913.3.
YP_492185.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.
GeneIDi12932725.
948543.
KEGGiecj:Y75_p3929.
eco:b4042.
PATRICi32123621. VBIEscCol129921_4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00127 Genomic DNA. Translation: AAA24394.1.
U00006 Genomic DNA. Translation: AAC43136.1.
U00096 Genomic DNA. Translation: AAC77012.1.
AP009048 Genomic DNA. Translation: BAE78044.1.
PIRiA00667. KIECDG.
RefSeqiNP_418466.1. NC_000913.3.
YP_492185.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KDCNMR-A/B/C2-122[»]
3ZE3X-ray2.05A/B/C/D/E/F2-122[»]
3ZE4X-ray3.70A/B/C2-122[»]
3ZE5X-ray3.10A/B/C2-122[»]
4BPDX-ray3.30A/B/C/D/E/F2-122[»]
4BRBX-ray2.55A/B/C/D/E/F2-122[»]
4BRRX-ray2.44A/B/C/D/E/F2-122[»]
4CJZX-ray3.25A/B/C2-122[»]
4CK0X-ray2.92A/B/C2-122[»]
4D2EX-ray2.28A/B/C/D/E/F2-122[»]
4UP6X-ray3.80A/B/C2-122[»]
ProteinModelPortaliP0ABN1.
SMRiP0ABN1. Positions 7-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi852837. 2 interactions.
DIPiDIP-60228N.
STRINGi511145.b4042.

Proteomic databases

PaxDbiP0ABN1.
PRIDEiP0ABN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77012; AAC77012; b4042.
BAE78044; BAE78044; BAE78044.
GeneIDi12932725.
948543.
KEGGiecj:Y75_p3929.
eco:b4042.
PATRICi32123621. VBIEscCol129921_4159.

Organism-specific databases

EchoBASEiEB0220.
EcoGeneiEG10224. dgkA.

Phylogenomic databases

eggNOGiCOG0818.
HOGENOMiHOG000014819.
InParanoidiP0ABN1.
KOiK00901.
OMAiIACFLDV.
OrthoDBiEOG60W7XM.
PhylomeDBiP0ABN1.

Enzyme and pathway databases

BioCyciEcoCyc:DIACYLGLYKIN-MONOMER.
ECOL316407:JW4002-MONOMER.
MetaCyc:DIACYLGLYKIN-MONOMER.
BRENDAi2.7.1.107. 2026.

Miscellaneous databases

EvolutionaryTraceiP0ABN1.
PROiP0ABN1.

Gene expression databases

GenevestigatoriP0ABN1.

Family and domain databases

InterProiIPR000829. Diacylglycerol_kinase_prok.
[Graphical view]
PfamiPF01219. DAGK_prokar. 1 hit.
[Graphical view]
PROSITEiPS01069. DAGK_PROKAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequences encoding plsB and dgk loci of Escherichia coli."
    Lightner V.A., Bell R.M., Modrich P.
    J. Biol. Chem. 258:10856-10861(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis."
    Loomis C.R., Walsh J.P., Bell R.M.
    J. Biol. Chem. 260:4091-4097(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 2-3 AND 121.
  6. "Membrane topology of Escherichia coli diacylglycerol kinase."
    Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II
    J. Bacteriol. 176:5459-5465(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiKDGL_ECOLI
AccessioniPrimary (citable) accession number: P0ABN1
Secondary accession number(s): P00556, Q2M6R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.