Reviewed, UniProtKB/Swiss-Prot P0ABM1 (CCMC_ECOLI)

Names and origin

Protein names

Recommended name:
    Heme exporter protein C
Alternative name(s):
    Cytochrome c-type biogenesis protein ccmC

Gene names

Name:	ccmC
Synonyms:	yejT, yejU
Ordered Locus Names:	b2199, JW2187

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ccmC/cycZ/helC family.
Sequence caution	The sequence AAA16391.1 differs from that shown. Reason: Frameshift at position 156.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW heme transport Inferred from electronic annotation. Source: InterPro respiratory chain complex IV assembly Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ccmE	P69490	1	EBI-2123469,EBI-1128007

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Heme exporter protein C		PRO_0000201551
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Potential
	Topological domain	42 – 63	22	Periplasmic Potential
	Transmembrane	64 – 84	21	Potential
	Topological domain	85 – 90	6	Cytoplasmic Potential
	Transmembrane	91 – 111	21	Potential
	Topological domain	112 – 128	17	Periplasmic Potential
	Transmembrane	129 – 149	21	Potential
	Topological domain	150 – 159	10	Cytoplasmic Potential
	Transmembrane	160 – 180	21	Potential
	Topological domain	181 – 205	25	Periplasmic Potential
	Transmembrane	206 – 226	21	Potential
	Topological domain	227 – 245	19	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ABM1-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: A98F2F754948EA60	FASTA	245	27,885
	10 20 30 40 50 60 MWKTLHQLAI PPRLYQICGW FIPWLAIASV VVLTVGWIWG FGFAPADYQQ GNSYRIIYLH 70 80 90 100 110 120 VPAAIWSMGI YASMAVAAFI GLVWQMKMAN LAVAAMAPIG AVFTFIALVT GSAWGKPMWG 130 140 150 160 170 180 TWWVWDARLT SELVLLFLYV GVIALWHAFD DRRLAGRAAG ILVLIGVVNL PIIHYSVEWW 190 200 210 220 230 240 NTLHQGSTRM QQSIDPAMRS PLRWSIFGFL LLSATLTLMR MRNLILLMEK RRPWVSELIL KRGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"Escherichia coli genes required for cytochrome c maturation." Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H. J. Bacteriol. 177:4321-4326(1995) [PubMed: 7635817] [Abstract] Cited for: SEQUENCE REVISION, CHARACTERIZATION, GENE NAME.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00008 Genomic DNA. Translation: AAA16391.1. Frameshift. U00096 Genomic DNA. Translation: AAC75259.1. AP009048 Genomic DNA. Translation: BAE76662.1.
PIR	E64989.
RefSeq	AP_002795.1. NP_416703.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ABM1. 1 interaction.
STRING	P0ABM1.
Protein family/group databases
TCDB	3.A.1.107.3. ATP-binding cassette (ABC) superfamily. 9.B.14.2.3. putative heme handling protein (HHP) family.
Genome annotation databases
GeneID	946703.
GenomeReviews	Gene locus JW2187 in contig AP009048_GR. Gene locus b2199 in contig U00096_GR.
KEGG	ecj:JW2187. eco:b2199.
Organism-specific databases
EchoBASE	EB1987.
EcoGene	EG12057. ccmC.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG589920.
OMA	TVRIMYV.
Enzyme and pathway databases
BioCyc	EcoCyc:CCMC-MON. ECOL168927:B2199-MON.
Gene expression databases
Genevestigator	P0ABM1.
Family and domain databases
InterPro	IPR002541. Cyt_c_assembly. IPR003557. Cyt_c_biogenesis_CcmC. [Graphical view]
Pfam	PF01578. Cytochrom_C_asm. 1 hit. [Graphical view]
PRINTS	PR01386. CCMCBIOGNSIS.
ProtoNet	Search...

Entry information

Entry name

CCMC_ECOLI

Accession

Primary (citable) accession number: P0ABM1
Secondary accession number(s): P33929, Q2MAP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	December 15, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)