ID   CCMB_ECOLI              Reviewed;         220 AA.
AC   P0ABL8; P33930; Q2MAP3;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Heme exporter protein B;
DE   AltName: Full=Cytochrome c-type biogenesis protein CcmB;
GN   Name=ccmB; Synonyms=yejV; OrderedLocusNames=b2200, JW2188;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / BHB2600;
RA   Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA   Church G.M.;
RT   "Automated multiplex sequencing of the E.coli genome.";
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   CHARACTERIZATION, AND GENE NAME.
RX   PubMed=7635817; DOI=10.1128/jb.177.15.4321-4326.1995;
RA   Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H.;
RT   "Escherichia coli genes required for cytochrome c maturation.";
RL   J. Bacteriol. 177:4321-4326(1995).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Required for the export of heme to the periplasm for the
CC       biogenesis of c-type cytochromes.
CC   -!- INTERACTION:
CC       P0ABL8; P33931: ccmA; NbExp=2; IntAct=EBI-546936, EBI-6402796;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the CcmB/CycW/HelB family. {ECO:0000305}.
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DR   EMBL; U00008; AAA16392.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75260.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76663.1; -; Genomic_DNA.
DR   PIR; F64989; F64989.
DR   RefSeq; NP_416704.1; NC_000913.3.
DR   RefSeq; WP_000971730.1; NZ_LN832404.1.
DR   PDB; 7F02; EM; 3.24 A; B/F=1-220.
DR   PDB; 7F03; EM; 3.29 A; B/F=1-220.
DR   PDB; 7F04; EM; 2.86 A; B/F=1-220.
DR   PDB; 7VFJ; EM; 3.98 A; B/F=1-220.
DR   PDB; 7VFP; EM; 4.03 A; B/F=1-220.
DR   PDB; 8CE1; EM; 3.47 A; B/b=1-220.
DR   PDB; 8CE5; EM; 3.62 A; B/b=1-220.
DR   PDB; 8CE8; EM; 3.81 A; B/b=1-220.
DR   PDB; 8CEA; EM; 3.94 A; B/b=1-220.
DR   PDBsum; 7F02; -.
DR   PDBsum; 7F03; -.
DR   PDBsum; 7F04; -.
DR   PDBsum; 7VFJ; -.
DR   PDBsum; 7VFP; -.
DR   PDBsum; 8CE1; -.
DR   PDBsum; 8CE5; -.
DR   PDBsum; 8CE8; -.
DR   PDBsum; 8CEA; -.
DR   AlphaFoldDB; P0ABL8; -.
DR   EMDB; EMD-16597; -.
DR   EMDB; EMD-16599; -.
DR   EMDB; EMD-16601; -.
DR   EMDB; EMD-16602; -.
DR   EMDB; EMD-31394; -.
DR   EMDB; EMD-31395; -.
DR   EMDB; EMD-31396; -.
DR   EMDB; EMD-31956; -.
DR   EMDB; EMD-31957; -.
DR   SMR; P0ABL8; -.
DR   BioGRID; 4260485; 8.
DR   ComplexPortal; CPX-3568; CcmABCDE system I cytochrome c biogenesis complex.
DR   DIP; DIP-48046N; -.
DR   IntAct; P0ABL8; 11.
DR   STRING; 511145.b2200; -.
DR   PaxDb; 511145-b2200; -.
DR   EnsemblBacteria; AAC75260; AAC75260; b2200.
DR   GeneID; 75206452; -.
DR   GeneID; 946692; -.
DR   KEGG; ecj:JW2188; -.
DR   KEGG; eco:b2200; -.
DR   PATRIC; fig|1411691.4.peg.36; -.
DR   EchoBASE; EB1988; -.
DR   eggNOG; COG2386; Bacteria.
DR   HOGENOM; CLU_079069_1_0_6; -.
DR   InParanoid; P0ABL8; -.
DR   OMA; IGPGILW; -.
DR   OrthoDB; 9799895at2; -.
DR   PhylomeDB; P0ABL8; -.
DR   BioCyc; EcoCyc:CCMB-MONOMER; -.
DR   BioCyc; MetaCyc:CCMB-MONOMER; -.
DR   PRO; PR:P0ABL8; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; NAS:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015232; F:heme transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:1903607; P:cytochrome c biosynthetic process; IDA:EcoCyc.
DR   GO; GO:0017004; P:cytochrome complex assembly; NAS:ComplexPortal.
DR   GO; GO:1904334; P:heme import across plasma membrane; NAS:ComplexPortal.
DR   InterPro; IPR003544; Cyt_c_biogenesis_CcmB.
DR   InterPro; IPR026031; Cyt_c_CcmB_bac.
DR   NCBIfam; TIGR01190; ccmB; 1.
DR   PANTHER; PTHR30070:SF1; CYTOCHROME C BIOGENESIS B-RELATED; 1.
DR   PANTHER; PTHR30070; HEME EXPORTER PROTEIN B; 1.
DR   Pfam; PF03379; CcmB; 1.
DR   PIRSF; PIRSF002764; CcmB; 1.
DR   PRINTS; PR01414; CCMBBIOGNSIS.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane;
KW   Cytochrome c-type biogenesis; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..220
FT                   /note="Heme exporter protein B"
FT                   /id="PRO_0000201539"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        42..44
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        66..100
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        122..127
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        149..158
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        180..192
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        193..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        214..220
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   220 AA;  23619 MW;  8DC1EEB8168401A3 CRC64;
     MMFWRIFRLE LRVAFRHSAE IANPLWFFLI VITLFPLSIG PEPQLLARIA PGIIWVAALL
     SSLLALERLF RDDLQDGSLE QLMLLPLPLP AVVLAKVMAH WMVTGLPLLI LSPLVAMLLG
     MDVYGWQVMA LTLLLGTPTL GFLGAPGVAL TVGLKRGGVL LSILVLPLTI PLLIFATAAM
     DAASMHLPVD GYLAILGALL AGTATLSPFA TAAALRISIQ
//