P0ABL8 (CCMB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme exporter protein B

Alternative name(s):
Cytochrome c-type biogenesis protein CcmB

Gene names

Name:	ccmB
Synonyms:	yejV
Ordered Locus Names:	b2200, JW2188

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CcmB/CycW/HelB family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from mutant phenotype PubMed 16824107. Source: EcoCyc integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme-transporting ATPase activity Inferred from mutant phenotype PubMed 17419738. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Heme exporter protein B		PRO_0000201539
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 44	3	Periplasmic Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Topological domain	66 – 100	35	Cytoplasmic Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Topological domain	122 – 127	6	Periplasmic Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Topological domain	149 – 158	10	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Topological domain	180 – 192	13	Periplasmic Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Topological domain	214 – 220	7	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ABL8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8DC1EEB8168401A3	FASTA	220	23,619
	10 20 30 40 50 60 MMFWRIFRLE LRVAFRHSAE IANPLWFFLI VITLFPLSIG PEPQLLARIA PGIIWVAALL 70 80 90 100 110 120 SSLLALERLF RDDLQDGSLE QLMLLPLPLP AVVLAKVMAH WMVTGLPLLI LSPLVAMLLG 130 140 150 160 170 180 MDVYGWQVMA LTLLLGTPTL GFLGAPGVAL TVGLKRGGVL LSILVLPLTI PLLIFATAAM 190 200 210 220 DAASMHLPVD GYLAILGALL AGTATLSPFA TAAALRISIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Escherichia coli genes required for cytochrome c maturation." Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H. J. Bacteriol. 177:4321-4326(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, GENE NAME.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00008 Genomic DNA. Translation: AAA16392.1. U00096 Genomic DNA. Translation: AAC75260.1. AP009048 Genomic DNA. Translation: BAE76663.1.
PIR	F64989.
RefSeq	NP_416704.1. NC_000913.2. YP_490438.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ABL8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48046N.
IntAct	P0ABL8. 9 interactions.
MINT	MINT-1250139.
STRING	511145.b2200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75260; AAC75260; b2200. BAE76663; BAE76663; BAE76663.
GeneID	12931491. 946692.
KEGG	ecj:Y75_p2161. eco:b2200.
PATRIC	32119759. VBIEscCol129921_2289.
Organism-specific databases
EchoBASE	EB1988.
EcoGene	EG12058. ccmB.
Phylogenomic databases
eggNOG	COG2386.
HOGENOM	HOG000274626.
KO	K02194.
OMA	WLVTGLP.
ProtClustDB	CLSK880332.
Enzyme and pathway databases
BioCyc	EcoCyc:CCMB-MONOMER. ECOL316407:JW2188-MONOMER.
Gene expression databases
Genevestigator	P0ABL8.
Family and domain databases
InterPro	IPR003544. Cyt_c_biogenesis_CcmB. IPR026031. Cyt_c_CcmB_bac. [Graphical view]
Pfam	PF03379. CcmB. 1 hit. [Graphical view]
PIRSF	PIRSF002764. CcmB. 1 hit.
PRINTS	PR01414. CCMBBIOGNSIS.
TIGRFAMs	TIGR01190. ccmB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCMB_ECOLI

Accession

Primary (citable) accession number: P0ABL8
Secondary accession number(s): P33930, Q2MAP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program