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Protein

Cytochrome c-type protein NrfB

Gene

nrfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in nitrite reduction.

Pathwayi: nitrogen metabolism

This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491Heme 1 (covalent)By similarity
Binding sitei52 – 521Heme 1 (covalent)By similarity
Metal bindingi53 – 531Iron (heme 1 axial ligand)By similarity
Binding sitei78 – 781Heme 2 (covalent)By similarity
Binding sitei81 – 811Heme 2 (covalent)By similarity
Metal bindingi82 – 821Iron (heme 2 axial ligand)By similarity
Binding sitei113 – 1131Heme 3 (covalent)By similarity
Binding sitei116 – 1161Heme 3 (covalent)By similarity
Metal bindingi117 – 1171Iron (heme 3 axial ligand)By similarity
Binding sitei138 – 1381Heme 4 (covalent)By similarity
Binding sitei141 – 1411Heme 4 (covalent)By similarity
Metal bindingi142 – 1421Iron (heme 4 axial ligand)By similarity
Binding sitei163 – 1631Heme 5 (covalent)By similarity
Binding sitei166 – 1661Heme 5 (covalent)By similarity
Metal bindingi167 – 1671Iron (heme 5 axial ligand)By similarity

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • heme binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • nitrite reductase (cytochrome, ammonia-forming) activity Source: EcoCyc

GO - Biological processi

  • anaerobic electron transport chain Source: EcoCyc
  • nitrogen compound metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYTOCHROMEC-MONOMER.
ECOL316407:JW4032-MONOMER.
MetaCyc:CYTOCHROMEC-MONOMER.
UniPathwayiUPA00045.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type protein NrfB
Gene namesi
Name:nrfB
Synonyms:yjcI
Ordered Locus Names:b4071, JW4032
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11945. nrfB.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence analysisAdd
BLAST
Chaini33 – 188156Cytochrome c-type protein NrfBPRO_0000006585Add
BLAST

Post-translational modificationi

Binds 5 heme groups per subunit.By similarity

Proteomic databases

PaxDbiP0ABL1.
PRIDEiP0ABL1.

Interactioni

Protein-protein interaction databases

BioGridi4259404. 7 interactions.
IntActiP0ABL1. 3 interactions.
STRINGi511145.b4071.

Structurei

Secondary structure

1
188
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi46 – 494Combined sources
Turni50 – 523Combined sources
Turni55 – 573Combined sources
Helixi63 – 653Combined sources
Turni70 – 723Combined sources
Beta strandi73 – 753Combined sources
Helixi78 – 825Combined sources
Helixi89 – 913Combined sources
Beta strandi94 – 963Combined sources
Beta strandi99 – 1013Combined sources
Helixi106 – 1138Combined sources
Turni114 – 1163Combined sources
Helixi119 – 1257Combined sources
Helixi129 – 1324Combined sources
Turni133 – 1353Combined sources
Helixi138 – 1403Combined sources
Beta strandi145 – 1484Combined sources
Helixi150 – 1534Combined sources
Helixi158 – 17215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZYX-ray1.74A26-188[»]
2P0BX-ray1.74A26-188[»]
ProteinModelPortaliP0ABL1.
SMRiP0ABL1. Positions 44-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABL1.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105JJ8. Bacteria.
ENOG411128Q. LUCA.
HOGENOMiHOG000278525.
InParanoidiP0ABL1.
KOiK04013.
OMAiDHREGAP.
PhylomeDBiP0ABL1.

Family and domain databases

InterProiIPR023155. Cyt_c-552/4.
IPR017564. Cyt_c_NO2Rdtase_pentahaem-su.
IPR011031. Multihaem_cyt.
IPR010177. Paired_CXXCH_1.
[Graphical view]
PfamiPF13435. Cytochrome_C554. 1 hit.
PF09699. Paired_CXXCH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48695. SSF48695. 1 hit.
TIGRFAMsiTIGR03146. cyt_nit_nrfB. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLRSLLTA GVLASGLLWS LNGITATPAA QASDDRYEVT QQRNPDAACL
60 70 80 90 100
DCHKPDTEGM HGKHASVINP NNKLPVTCTN CHGQPSPQHR EGVKDVMRFN
110 120 130 140 150
EPMYKVGEQN SVCMSCHLPE QLQKAFWPHD VHVTKVACAS CHSLHPQQDT
160 170 180
MQTLSDKGRI KICVDCHSDQ RTNPNFNPAS VPLLKEQP
Length:188
Mass (Da):20,714
Last modified:November 8, 2005 - v1
Checksum:i33A354F20FAF1548
GO

Sequence cautioni

The sequence AAC43165 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51042.1.
U00006 Genomic DNA. Translation: AAC43165.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77041.2.
AP009048 Genomic DNA. Translation: BAE78073.1.
RefSeqiNP_418495.2. NC_000913.3.
WP_001295391.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77041; AAC77041; b4071.
BAE78073; BAE78073; BAE78073.
GeneIDi948573.
KEGGiecj:JW4032.
eco:b4071.
PATRICi32123691. VBIEscCol129921_4194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51042.1.
U00006 Genomic DNA. Translation: AAC43165.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77041.2.
AP009048 Genomic DNA. Translation: BAE78073.1.
RefSeqiNP_418495.2. NC_000913.3.
WP_001295391.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZYX-ray1.74A26-188[»]
2P0BX-ray1.74A26-188[»]
ProteinModelPortaliP0ABL1.
SMRiP0ABL1. Positions 44-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259404. 7 interactions.
IntActiP0ABL1. 3 interactions.
STRINGi511145.b4071.

Proteomic databases

PaxDbiP0ABL1.
PRIDEiP0ABL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77041; AAC77041; b4071.
BAE78073; BAE78073; BAE78073.
GeneIDi948573.
KEGGiecj:JW4032.
eco:b4071.
PATRICi32123691. VBIEscCol129921_4194.

Organism-specific databases

EchoBASEiEB1888.
EcoGeneiEG11945. nrfB.

Phylogenomic databases

eggNOGiENOG4105JJ8. Bacteria.
ENOG411128Q. LUCA.
HOGENOMiHOG000278525.
InParanoidiP0ABL1.
KOiK04013.
OMAiDHREGAP.
PhylomeDBiP0ABL1.

Enzyme and pathway databases

UniPathwayiUPA00045.
BioCyciEcoCyc:CYTOCHROMEC-MONOMER.
ECOL316407:JW4032-MONOMER.
MetaCyc:CYTOCHROMEC-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ABL1.
PROiP0ABL1.

Family and domain databases

InterProiIPR023155. Cyt_c-552/4.
IPR017564. Cyt_c_NO2Rdtase_pentahaem-su.
IPR011031. Multihaem_cyt.
IPR010177. Paired_CXXCH_1.
[Graphical view]
PfamiPF13435. Cytochrome_C554. 1 hit.
PF09699. Paired_CXXCH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48695. SSF48695. 1 hit.
TIGRFAMsiTIGR03146. cyt_nit_nrfB. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRFB_ECOLI
AccessioniPrimary (citable) accession number: P0ABL1
Secondary accession number(s): P32707, P76790, Q2M6N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.