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Protein

Cysteine synthase A

Gene

cysK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In addition to its role in cysteine synthesis, stimulates the tRNase activity of CdiA-CT from E.coli strain 536 / UPEC; stimulation does not require O-acetylserine sulfhydrylase activity. CdiA is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (experiments done in strains BW25113 and X90, both K12 derivatives). This protein is not required for CDI of strain EC93, whose toxin may function by forming inner cell membrane pores.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication
3-chloro-L-alanine + thioglycolate = S-carboxymethyl-L-cysteine + chloride.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=40 mM for 3-chloro-L-alanine1 Publication
  2. KM=15.4 mM for thioglycolate1 Publication

    pH dependencei

    Optimum pH is 9-10.5.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase (cysE)
    2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81Allosteric inhibitorBy similarity
    Binding sitei72 – 721Pyridoxal phosphateBy similarity
    Binding sitei269 – 2691Allosteric inhibitor; via amide nitrogenBy similarity
    Binding sitei273 – 2731Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    • cysteine synthase activity Source: EcoliWiki
    • L-cysteine desulfhydrase activity Source: EcoCyc
    • pyridoxal phosphate binding Source: EcoCyc
    • S-carboxymethylcysteine synthase activity Source: UniProtKB-EC
    • transferase activity Source: EcoliWiki

    GO - Biological processi

    • cellular amino acid biosynthetic process Source: EcoliWiki
    • cysteine biosynthetic process from serine Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:ACSERLYA-MONOMER.
    ECOL316407:JW2407-MONOMER.
    MetaCyc:ACSERLYA-MONOMER.
    SABIO-RKP0ABK5.
    UniPathwayiUPA00136; UER00200.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine synthase A (EC:2.5.1.47)
    Short name:
    CSase A
    Alternative name(s):
    O-acetylserine (thiol)-lyase A
    Short name:
    OAS-TL A
    O-acetylserine sulfhydrylase A
    S-carboxymethylcysteine synthase (EC:4.5.1.51 Publication)
    Sulfate starvation-induced protein 5
    Short name:
    SSI5
    Gene namesi
    Name:cysK
    Synonyms:cysZ
    Ordered Locus Names:b2414, JW2407
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10192. cysK.

    Subcellular locationi

    GO - Cellular componenti

    • cysteine synthase complex Source: EcoCyc
    • cytosol Source: UniProtKB
    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Significant decrease in tRNA nuclease activity of contact-dependent growth inhibitor CdiA-CT from strain 536 / UPEC (deletion in strain BW25113) (PubMed:22333533).1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi42 – 421K → A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved5 Publications
    Chaini2 – 323322Cysteine synthase APRO_0000167086Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    EPDiP0ABK5.
    PaxDbiP0ABK5.
    PRIDEiP0ABK5.

    2D gel databases

    SWISS-2DPAGEP0ABK5.

    Expressioni

    Inductioni

    Repressed by sulfate or cysteine.

    Interactioni

    Subunit structurei

    Homodimer (Ref. 6). Forms a cysteine synthase complex with 1 copy of CysE (By similarity). Interacts with CdiA-CT from strain 536 / UPEC, this is blocked upon preincubation with O-acetyl-L-serine. CysK forms a complex with CdiA-CT/CdiI (PubMed:22333533).By similarity2 Publications

    Protein-protein interaction databases

    BioGridi4259701. 7 interactions.
    DIPiDIP-36170N.
    IntActiP0ABK5. 14 interactions.
    MINTiMINT-1240013.
    STRINGi511145.b2414.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ABK5.
    SMRiP0ABK5. Positions 2-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni177 – 1815Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C6T. Bacteria.
    COG0031. LUCA.
    HOGENOMiHOG000217394.
    InParanoidiP0ABK5.
    KOiK01738.
    OMAiVKCRIGS.
    PhylomeDBiP0ABK5.

    Family and domain databases

    InterProiIPR005856. Cys_synth.
    IPR005859. CysK.
    IPR001216. P-phosphate_BS.
    IPR001926. TrpB-like_PLP-dep.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01139. cysK. 1 hit.
    TIGR01136. cysKM. 1 hit.
    PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0ABK5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKIFEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI
    60 70 80 90 100
    WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER
    110 120 130 140 150
    RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASNPEKYLL LQQFSNPANP
    160 170 180 190 200
    EIHEKTTGPE IWEDTDGQVD VFIAGVGTGG TLTGVSRYIK GTKGKTDLIS
    210 220 230 240 250
    VAVEPTDSPV IAQALAGEEI KPGPHKIQGI GAGFIPANLD LKLVDKVIGI
    260 270 280 290 300
    TNEEAISTAR RLMEEEGILA GISSGAAVAA ALKLQEDESF TNKNIVVILP
    310 320
    SSGERYLSTA LFADLFTEKE LQQ
    Length:323
    Mass (Da):34,490
    Last modified:January 23, 2007 - v2
    Checksum:i20ADDE911845AE72
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1821L → W in AAA23654 (PubMed:3290198).Curated
    Sequence conflicti186 – 1872SR → TP in AAA23654 (PubMed:3290198).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X12615 Genomic DNA. Translation: CAA31137.1.
    M21451 Genomic DNA. Translation: AAA23654.1.
    U00096 Genomic DNA. Translation: AAC75467.1.
    AP009048 Genomic DNA. Translation: BAA16288.1.
    M21994 Genomic DNA. Translation: AAA24383.1.
    PIRiE65015. SYECAC.
    RefSeqiNP_416909.1. NC_000913.3.
    WP_000034402.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75467; AAC75467; b2414.
    BAA16288; BAA16288; BAA16288.
    GeneIDi946877.
    KEGGiecj:JW2407.
    eco:b2414.
    PATRICi32120211. VBIEscCol129921_2508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X12615 Genomic DNA. Translation: CAA31137.1.
    M21451 Genomic DNA. Translation: AAA23654.1.
    U00096 Genomic DNA. Translation: AAC75467.1.
    AP009048 Genomic DNA. Translation: BAA16288.1.
    M21994 Genomic DNA. Translation: AAA24383.1.
    PIRiE65015. SYECAC.
    RefSeqiNP_416909.1. NC_000913.3.
    WP_000034402.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0ABK5.
    SMRiP0ABK5. Positions 2-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259701. 7 interactions.
    DIPiDIP-36170N.
    IntActiP0ABK5. 14 interactions.
    MINTiMINT-1240013.
    STRINGi511145.b2414.

    2D gel databases

    SWISS-2DPAGEP0ABK5.

    Proteomic databases

    EPDiP0ABK5.
    PaxDbiP0ABK5.
    PRIDEiP0ABK5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75467; AAC75467; b2414.
    BAA16288; BAA16288; BAA16288.
    GeneIDi946877.
    KEGGiecj:JW2407.
    eco:b2414.
    PATRICi32120211. VBIEscCol129921_2508.

    Organism-specific databases

    EchoBASEiEB0189.
    EcoGeneiEG10192. cysK.

    Phylogenomic databases

    eggNOGiENOG4105C6T. Bacteria.
    COG0031. LUCA.
    HOGENOMiHOG000217394.
    InParanoidiP0ABK5.
    KOiK01738.
    OMAiVKCRIGS.
    PhylomeDBiP0ABK5.

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00200.
    BioCyciEcoCyc:ACSERLYA-MONOMER.
    ECOL316407:JW2407-MONOMER.
    MetaCyc:ACSERLYA-MONOMER.
    SABIO-RKP0ABK5.

    Miscellaneous databases

    PROiP0ABK5.

    Family and domain databases

    InterProiIPR005856. Cys_synth.
    IPR005859. CysK.
    IPR001216. P-phosphate_BS.
    IPR001926. TrpB-like_PLP-dep.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01139. cysK. 1 hit.
    TIGR01136. cysKM. 1 hit.
    PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYSK_ECOLI
    AccessioniPrimary (citable) accession number: P0ABK5
    Secondary accession number(s): P11096, P21546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: January 23, 2007
    Last modified: September 7, 2016
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.