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Protein

Cytochrome bo(3) ubiquinol oxidase subunit 3

Gene

cyoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.3 Publications

GO - Molecular functioni

  • cytochrome bo3 ubiquinol oxidase activity Source: EcoCyc
  • cytochrome-c oxidase activity Source: InterPro
  • cytochrome o ubiquinol oxidase activity Source: InterPro
  • electron carrier activity Source: EcoCyc
  • hydrogen ion transmembrane transporter activity Source: EcoCyc
  • oxidoreduction-driven active transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • aerobic electron transport chain Source: EcoCyc
  • aerobic respiration Source: EcoCyc
  • electron transport coupled proton transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CYOC-MONOMER.
ECOL316407:JW0420-MONOMER.
MetaCyc:CYOC-MONOMER.
RETL1328306-WGS:GSTH-5876-MONOMER.

Protein family/group databases

TCDBi3.D.4.5.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 3
Alternative name(s):
Cytochrome o ubiquinol oxidase subunit 3
Short name:
Cytochrome o subunit 3
Oxidase bo(3) subunit 3
Ubiquinol oxidase chain C
Ubiquinol oxidase polypeptide III
Ubiquinol oxidase subunit 3
Gene namesi
Name:cyoC
Ordered Locus Names:b0430, JW0420
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10180. cyoC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicCuratedAdd
BLAST
Transmembranei32 – 5019HelicalCuratedAdd
BLAST
Topological domaini51 – 6616PeriplasmicCuratedAdd
BLAST
Transmembranei67 – 8519HelicalCuratedAdd
BLAST
Topological domaini86 – 10116CytoplasmicCuratedAdd
BLAST
Transmembranei102 – 12019HelicalCuratedAdd
BLAST
Topological domaini121 – 14222PeriplasmicCuratedAdd
BLAST
Transmembranei143 – 16119HelicalCuratedAdd
BLAST
Topological domaini162 – 18423CytoplasmicCuratedAdd
BLAST
Transmembranei185 – 20319HelicalCuratedAdd
BLAST
Topological domaini204 – 2041PeriplasmicCurated

GO - Cellular componenti

  • cytochrome o ubiquinol oxidase complex Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased reduction of the ubiquinone pool (in aerobically grown minimal medium with glucose).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Cytochrome bo(3) ubiquinol oxidase subunit 3PRO_0000183893Add
BLAST

Proteomic databases

PaxDbiP0ABJ3.

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains.2 Publications

Protein-protein interaction databases

BioGridi4261578. 174 interactions.
DIPiDIP-47944N.
IntActiP0ABJ3. 1 interaction.
STRINGi511145.b0430.

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 306Combined sources
Helixi33 – 364Combined sources
Helixi39 – 479Combined sources
Turni48 – 536Combined sources
Helixi66 – 7611Combined sources
Helixi77 – 793Combined sources
Turni80 – 845Combined sources
Helixi86 – 883Combined sources
Turni89 – 924Combined sources
Helixi95 – 984Combined sources
Helixi100 – 12526Combined sources
Turni135 – 1373Combined sources
Helixi138 – 1469Combined sources
Helixi149 – 1524Combined sources
Helixi155 – 16612Combined sources
Helixi176 – 19419Combined sources
Helixi195 – 2028Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFTX-ray3.50C/H25-204[»]
ProteinModelPortaliP0ABJ3.
SMRiP0ABJ3. Positions 19-203.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABJ3.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CCU. Bacteria.
COG1845. LUCA.
HOGENOMiHOG000086398.
InParanoidiP0ABJ3.
KOiK02299.
OMAiYTELVKH.
OrthoDBiEOG6SFPBZ.
PhylomeDBiP0ABJ3.

Family and domain databases

Gene3Di1.20.120.80. 1 hit.
InterProiIPR024791. Cyt_c/ubiquinol_Oxase_su3.
IPR013833. Cyt_c_oxidase_su3_a-hlx.
IPR000298. Cyt_c_oxidase_su3_dom.
IPR014206. Cyt_c_ubiqinol_oxidase_su3.
[Graphical view]
PANTHERiPTHR11403. PTHR11403. 1 hit.
PfamiPF00510. COX3. 1 hit.
[Graphical view]
SUPFAMiSSF81452. SSF81452. 1 hit.
TIGRFAMsiTIGR02842. CyoC. 1 hit.
PROSITEiPS50253. COX3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ABJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDTLTHAT AHAHEHGHHD AGGTKIFGFW IYLMSDCILF SILFATYAVL
60 70 80 90 100
VNGTAGGPTG KDIFELPFVL VETFLLLFSS ITYGMAAIAM YKNNKSQVIS
110 120 130 140 150
WLALTWLFGA GFIGMEIYEF HHLIVNGMGP DRSGFLSAFF ALVGTHGLHV
160 170 180 190 200
TSGLIWMAVL MVQIARRGLT STNRTRIMCL SLFWHFLDVV WICVFTVVYL

MGAM
Length:204
Mass (Da):22,623
Last modified:October 25, 2005 - v1
Checksum:iF1D6ABF9EB6CB5AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23633.1.
U82664 Genomic DNA. Translation: AAB40186.1.
U00096 Genomic DNA. Translation: AAC73533.1.
AP009048 Genomic DNA. Translation: BAE76210.1.
PIRiC42226.
RefSeqiNP_414964.1. NC_000913.3.
WP_000179819.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73533; AAC73533; b0430.
BAE76210; BAE76210; BAE76210.
GeneIDi946897.
KEGGiecj:JW0420.
eco:b0430.
PATRICi32116011. VBIEscCol129921_0447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23633.1.
U82664 Genomic DNA. Translation: AAB40186.1.
U00096 Genomic DNA. Translation: AAC73533.1.
AP009048 Genomic DNA. Translation: BAE76210.1.
PIRiC42226.
RefSeqiNP_414964.1. NC_000913.3.
WP_000179819.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFTX-ray3.50C/H25-204[»]
ProteinModelPortaliP0ABJ3.
SMRiP0ABJ3. Positions 19-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261578. 174 interactions.
DIPiDIP-47944N.
IntActiP0ABJ3. 1 interaction.
STRINGi511145.b0430.

Protein family/group databases

TCDBi3.D.4.5.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

PaxDbiP0ABJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73533; AAC73533; b0430.
BAE76210; BAE76210; BAE76210.
GeneIDi946897.
KEGGiecj:JW0420.
eco:b0430.
PATRICi32116011. VBIEscCol129921_0447.

Organism-specific databases

EchoBASEiEB0177.
EcoGeneiEG10180. cyoC.

Phylogenomic databases

eggNOGiENOG4105CCU. Bacteria.
COG1845. LUCA.
HOGENOMiHOG000086398.
InParanoidiP0ABJ3.
KOiK02299.
OMAiYTELVKH.
OrthoDBiEOG6SFPBZ.
PhylomeDBiP0ABJ3.

Enzyme and pathway databases

BioCyciEcoCyc:CYOC-MONOMER.
ECOL316407:JW0420-MONOMER.
MetaCyc:CYOC-MONOMER.
RETL1328306-WGS:GSTH-5876-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ABJ3.
PROiP0ABJ3.

Family and domain databases

Gene3Di1.20.120.80. 1 hit.
InterProiIPR024791. Cyt_c/ubiquinol_Oxase_su3.
IPR013833. Cyt_c_oxidase_su3_a-hlx.
IPR000298. Cyt_c_oxidase_su3_dom.
IPR014206. Cyt_c_ubiqinol_oxidase_su3.
[Graphical view]
PANTHERiPTHR11403. PTHR11403. 1 hit.
PfamiPF00510. COX3. 1 hit.
[Graphical view]
SUPFAMiSSF81452. SSF81452. 1 hit.
TIGRFAMsiTIGR02842. CyoC. 1 hit.
PROSITEiPS50253. COX3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases."
    Chepuri V., Lemieux L., Au D.C.T., Gennis R.B.
    J. Biol. Chem. 265:11185-11192(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli."
    Matsushita K., Patel L., Gennis R.B., Kaback H.R.
    Proc. Natl. Acad. Sci. U.S.A. 80:4889-4893(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUINOL OXIDATION, FUNCTION IN PROTON ELECTROCHEMICAL GRADIENT GENERATION, SUBUNIT.
  6. "The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli."
    Chepuri V., Gennis R.B.
    J. Biol. Chem. 265:12978-12986(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Recent studies of the cytochrome o terminal oxidase complex of Escherichia coli."
    Chepuri V., Lemieux L., Hill J., Alben J.O., Gennis R.B.
    Biochim. Biophys. Acta 1018:124-127(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Respiration of Escherichia coli can be fully uncoupled via the nonelectrogenic terminal cytochrome bd-II oxidase."
    Bekker M., de Vries S., Ter Beek A., Hellingwerf K.J., de Mattos M.J.
    J. Bacteriol. 191:5510-5517(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN OXIDASE, FUNCTION AS A PROTON PUMP, DISRUPTION PHENOTYPE.
    Strain: K12.
  10. "Uncoupling of substrate-level phosphorylation in Escherichia coli during glucose-limited growth."
    Sharma P., Hellingwerf K.J., de Mattos M.J., Bekker M.
    Appl. Environ. Microbiol. 78:6908-6913(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN OXIDASE, FUNCTION IN PROTON TRANSLOCATION, DISRUPTION PHENOTYPE.
    Strain: K12.
  11. "The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site."
    Abramson J., Riistama S., Larsson G., Jasaitis A., Svensson-Ek M., Puustinen A., Iwata S., Wikstrom M.
    Nat. Struct. Biol. 7:910-917(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiCYOC_ECOLI
AccessioniPrimary (citable) accession number: P0ABJ3
Secondary accession number(s): P18402, Q2MBZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: January 20, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.