Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome bo(3) ubiquinol oxidase subunit 2

Gene

cyoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.3 Publications

Enzyme regulationi

Competitively inhibited by piericidin A, non-competitively inhibited by 2-n-heptyl-4-hydroxyquinoline N-oxide, NaN3 and KCN; 50% inhibition occurs at 2 µM, 2 µM, 15 mM and 10 µM, respectively. Inhibited by Zn2+ and Cd2+.1 Publication

GO - Molecular functioni

  • copper ion binding Source: InterPro
  • cytochrome bo3 ubiquinol oxidase activity Source: EcoCyc
  • cytochrome-c oxidase activity Source: InterPro
  • cytochrome o ubiquinol oxidase activity Source: InterPro
  • electron carrier activity Source: EcoCyc
  • hydrogen ion transmembrane transporter activity Source: EcoCyc
  • oxidoreduction-driven active transmembrane transporter activity Source: EcoCyc
  • ubiquinone binding Source: EcoCyc

GO - Biological processi

  • aerobic electron transport chain Source: EcoCyc
  • aerobic respiration Source: EcoCyc
  • ATP synthesis coupled electron transport Source: GO_Central
  • electron transport coupled proton transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CYOA-MONOMER.
ECOL316407:JW0422-MONOMER.
MetaCyc:CYOA-MONOMER.
BRENDAi1.10.3.10. 2026.

Protein family/group databases

TCDBi3.D.4.5.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 2
Alternative name(s):
Cytochrome b562-o complex subunit II
Cytochrome o ubiquinol oxidase subunit 2
Short name:
Cytochrome o subunit 2
Oxidase bo(3) subunit 2
Ubiquinol oxidase chain B
Ubiquinol oxidase polypeptide II
Ubiquinol oxidase subunit 2
Gene namesi
Name:cyoA
Ordered Locus Names:b0432, JW0422
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10178. cyoA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 50PeriplasmicCuratedAdd BLAST26
Transmembranei51 – 68HelicalCuratedAdd BLAST18
Topological domaini69 – 92CytoplasmicCuratedAdd BLAST24
Transmembranei93 – 111HelicalCuratedAdd BLAST19
Topological domaini112 – 315PeriplasmicCuratedAdd BLAST204

GO - Cellular componenti

  • cytochrome o ubiquinol oxidase complex Source: EcoCyc
  • integral component of external side of plasma membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased reduction of the ubiquinone pool (in aerobically grown minimal medium with glucose).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24PROSITE-ProRule annotationAdd BLAST24
ChainiPRO_000000607125 – 315Cytochrome bo(3) ubiquinol oxidase subunit 2Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi25N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi25S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0ABJ1.
PaxDbiP0ABJ1.
PRIDEiP0ABJ1.

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains.3 Publications

Protein-protein interaction databases

BioGridi4259512. 241 interactors.
DIPiDIP-47942N.
IntActiP0ABJ1. 23 interactors.
STRINGi511145.b0432.

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 50Combined sources14
Turni51 – 56Combined sources6
Helixi57 – 63Combined sources7
Turni64 – 71Combined sources8
Turni73 – 75Combined sources3
Helixi87 – 91Combined sources5
Helixi94 – 110Combined sources17
Beta strandi127 – 134Combined sources8
Beta strandi137 – 142Combined sources6
Turni143 – 146Combined sources4
Beta strandi147 – 156Combined sources10
Beta strandi161 – 170Combined sources10
Beta strandi172 – 176Combined sources5
Helixi177 – 179Combined sources3
Beta strandi181 – 185Combined sources5
Beta strandi191 – 195Combined sources5
Beta strandi198 – 207Combined sources10
Turni213 – 217Combined sources5
Beta strandi220 – 227Combined sources8
Helixi228 – 239Combined sources12
Helixi248 – 255Combined sources8
Beta strandi264 – 267Combined sources4
Helixi273 – 278Combined sources6
Turni279 – 281Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYWX-ray2.50A111-315[»]
1CYXX-ray2.30A111-315[»]
1FFTX-ray3.50B/G1-315[»]
DisProtiDP00089.
ProteinModelPortaliP0ABJ1.
SMRiP0ABJ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABJ1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E2S. Bacteria.
COG1622. LUCA.
HOGENOMiHOG000084157.
InParanoidiP0ABJ1.
KOiK02297.
OMAiTAMNSFF.
PhylomeDBiP0ABJ1.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR010514. COX_ARM.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
IPR006333. Cyt_o_ubiquinol_oxidase_su2.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF06481. COX_ARM. 1 hit.
[Graphical view]
PIRSFiPIRSF000292. Ubi_od_II. 1 hit.
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR01433. CyoA. 1 hit.
PROSITEiPS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRKYNKSL GWLSLFAGTV LLSGCNSALL DPKGQIGLEQ RSLILTAFGL
60 70 80 90 100
MLIVVIPAIL MAVGFAWKYR ASNKDAKYSP NWSHSNKVEA VVWTVPILII
110 120 130 140 150
IFLAVLTWKT THALEPSKPL AHDEKPITIE VVSMDWKWFF IYPEQGIATV
160 170 180 190 200
NEIAFPANTP VYFKVTSNSV MNSFFIPRLG SQIYAMAGMQ TRLHLIANEP
210 220 230 240 250
GTYDGISASY SGPGFSGMKF KAIATPDRAA FDQWVAKAKQ SPNTMSDMAA
260 270 280 290 300
FEKLAAPSEY NQVEYFSNVK PDLFADVINK FMAHGKSMDM TQPEGEHSAH
310
EGMEGMDMSH AESAH
Length:315
Mass (Da):34,911
Last modified:October 25, 2005 - v1
Checksum:i62EC951183B65724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23631.1.
U82664 Genomic DNA. Translation: AAB40188.1.
U00096 Genomic DNA. Translation: AAC73535.1.
AP009048 Genomic DNA. Translation: BAE76212.1.
M55258 Genomic DNA. Translation: AAA23630.1.
S67816 Genomic DNA. Translation: AAB28885.1.
PIRiA42226.
RefSeqiNP_414966.1. NC_000913.3.
WP_001239436.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73535; AAC73535; b0432.
BAE76212; BAE76212; BAE76212.
GeneIDi945080.
KEGGiecj:JW0422.
eco:b0432.
PATRICi32116015. VBIEscCol129921_0449.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23631.1.
U82664 Genomic DNA. Translation: AAB40188.1.
U00096 Genomic DNA. Translation: AAC73535.1.
AP009048 Genomic DNA. Translation: BAE76212.1.
M55258 Genomic DNA. Translation: AAA23630.1.
S67816 Genomic DNA. Translation: AAB28885.1.
PIRiA42226.
RefSeqiNP_414966.1. NC_000913.3.
WP_001239436.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYWX-ray2.50A111-315[»]
1CYXX-ray2.30A111-315[»]
1FFTX-ray3.50B/G1-315[»]
DisProtiDP00089.
ProteinModelPortaliP0ABJ1.
SMRiP0ABJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259512. 241 interactors.
DIPiDIP-47942N.
IntActiP0ABJ1. 23 interactors.
STRINGi511145.b0432.

Protein family/group databases

TCDBi3.D.4.5.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

EPDiP0ABJ1.
PaxDbiP0ABJ1.
PRIDEiP0ABJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73535; AAC73535; b0432.
BAE76212; BAE76212; BAE76212.
GeneIDi945080.
KEGGiecj:JW0422.
eco:b0432.
PATRICi32116015. VBIEscCol129921_0449.

Organism-specific databases

EchoBASEiEB0175.
EcoGeneiEG10178. cyoA.

Phylogenomic databases

eggNOGiENOG4105E2S. Bacteria.
COG1622. LUCA.
HOGENOMiHOG000084157.
InParanoidiP0ABJ1.
KOiK02297.
OMAiTAMNSFF.
PhylomeDBiP0ABJ1.

Enzyme and pathway databases

BioCyciEcoCyc:CYOA-MONOMER.
ECOL316407:JW0422-MONOMER.
MetaCyc:CYOA-MONOMER.
BRENDAi1.10.3.10. 2026.

Miscellaneous databases

EvolutionaryTraceiP0ABJ1.
PROiP0ABJ1.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR010514. COX_ARM.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
IPR006333. Cyt_o_ubiquinol_oxidase_su2.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF06481. COX_ARM. 1 hit.
[Graphical view]
PIRSFiPIRSF000292. Ubi_od_II. 1 hit.
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR01433. CyoA. 1 hit.
PROSITEiPS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYOA_ECOLI
AccessioniPrimary (citable) accession number: P0ABJ1
Secondary accession number(s): P18400, Q2MBZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.