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Protein

Magnesium transport protein CorA

Gene

corA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates influx of magnesium ions. Can also mediate cobalt and manganese uptake (PubMed:780341). Alternates between open and closed states. Activated by low cytoplasmic Mg2+ levels. Inactive when cytoplasmic Mg2+ levels are high (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei253 – 2531Essential for ion permeationBy similarity

GO - Molecular functioni

  • cobalt ion transmembrane transporter activity Source: EcoCyc
  • magnesium ion transmembrane transporter activity Source: EcoCyc
  • nickel cation transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cobalt ion transport Source: EcoCyc
  • magnesium ion transmembrane transport Source: GOC
  • magnesium ion transport Source: EcoCyc
  • nickel cation transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Cobalt, Magnesium, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:CORA-MONOMER.
ECOL316407:JW3789-MONOMER.
MetaCyc:CORA-MONOMER.

Protein family/group databases

TCDBi1.A.35.1.1. the cora metal ion transporter (mit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium transport protein CorA
Gene namesi
Name:corA
Ordered Locus Names:b3816, JW3789
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11463. corA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 254254CytoplasmicSequence analysisAdd
BLAST
Transmembranei255 – 27319HelicalSequence analysisAdd
BLAST
Topological domaini274 – 28714PeriplasmicSequence analysisAdd
BLAST
Transmembranei288 – 31023HelicalSequence analysisAdd
BLAST
Topological domaini311 – 3166CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Magnesium transport protein CorAPRO_0000201527Add
BLAST

Proteomic databases

EPDiP0ABI4.
PaxDbiP0ABI4.
PRIDEiP0ABI4.

Interactioni

Subunit structurei

Homopentamer. In the absence of Mg2+, interactions between subunits are weakened, and dimers, trimers and tetramers can be observed in vitro (By similarity).By similarity

Protein-protein interaction databases

BioGridi4263113. 3 interactions.
IntActiP0ABI4. 4 interactions.
STRINGi511145.b3816.

Structurei

3D structure databases

ProteinModelPortaliP0ABI4.
SMRiP0ABI4. Positions 27-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi277 – 2793Probable selectivity filterBy similarity

Domaini

The central ion permeation pathway is formed by the first transmembrane domain from each of the five subunits. Mg2+ binding strengthens interactions between subunits and leads to the formation of a symmetrical homopentamer surrounding a closed ion permeation pathway. Co2+ binding also induces a conformation change. Low Mg2+ concentrations trigger both a conformation change within each subunit and a loosening of the interactions between subunits. This results in an open ion conduction pathway. In addition, this results in a less symmetrical shape of the whole complex.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QUT. Bacteria.
COG0598. LUCA.
HOGENOMiHOG000276873.
InParanoidiP0ABI4.
KOiK03284.
OMAiRRAVSFM.
OrthoDBiEOG6MD91T.
PhylomeDBiP0ABI4.

Family and domain databases

InterProiIPR004488. Mg/Co-transport_prot_CorA.
IPR002523. MgTranspt_CorA/ZnTranspt_ZntB.
[Graphical view]
PfamiPF01544. CorA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00383. corA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSAFQLENN RLTRLEVEES QPLVNAVWID LVEPDDDERL RVQSELGQSL
60 70 80 90 100
ATRPELEDIE ASARFFEDDD GLHIHSFFFF EDAEDHAGNS TVAFTIRDGR
110 120 130 140 150
LFTLRERELP AFRLYRMRAR SQSMVDGNAY ELLLDLFETK IEQLADEIEN
160 170 180 190 200
IYSDLEQLSR VIMEGHQGDE YDEALSTLAE LEDIGWKVRL CLMDTQRALN
210 220 230 240 250
FLVRKARLPG GQLEQAREIL RDIESLLPHN ESLFQKVNFL MQAAMGFINI
260 270 280 290 300
EQNRIIKIFS VVSVVFLPPT LVASSYGMNF EFMPELKWSF GYPGAIIFMI
310
LAGLAPYLYF KRKNWL
Length:316
Mass (Da):36,590
Last modified:October 25, 2005 - v1
Checksum:iCC3B1B736EE36A53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 502SL → RP (PubMed:8314774).Curated
Sequence conflicti49 – 502SL → RP (PubMed:1379743).Curated
Sequence conflicti303 – 3031G → A in AAB59046 (PubMed:8314774).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11042 Genomic DNA. Translation: AAB59046.1.
L02122 Genomic DNA. Translation: AAD15038.1.
M87049 Genomic DNA. Translation: AAA67612.1.
U00096 Genomic DNA. Translation: AAC76819.1.
AP009048 Genomic DNA. Translation: BAE77485.1.
PIRiA65186. B47157.
RefSeqiNP_418260.1. NC_000913.3.
WP_000947159.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76819; AAC76819; b3816.
BAE77485; BAE77485; BAE77485.
GeneIDi948351.
KEGGiecj:JW3789.
eco:b3816.
PATRICi32123133. VBIEscCol129921_3932.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11042 Genomic DNA. Translation: AAB59046.1.
L02122 Genomic DNA. Translation: AAD15038.1.
M87049 Genomic DNA. Translation: AAA67612.1.
U00096 Genomic DNA. Translation: AAC76819.1.
AP009048 Genomic DNA. Translation: BAE77485.1.
PIRiA65186. B47157.
RefSeqiNP_418260.1. NC_000913.3.
WP_000947159.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABI4.
SMRiP0ABI4. Positions 27-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263113. 3 interactions.
IntActiP0ABI4. 4 interactions.
STRINGi511145.b3816.

Protein family/group databases

TCDBi1.A.35.1.1. the cora metal ion transporter (mit) family.

Proteomic databases

EPDiP0ABI4.
PaxDbiP0ABI4.
PRIDEiP0ABI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76819; AAC76819; b3816.
BAE77485; BAE77485; BAE77485.
GeneIDi948351.
KEGGiecj:JW3789.
eco:b3816.
PATRICi32123133. VBIEscCol129921_3932.

Organism-specific databases

EchoBASEiEB1431.
EcoGeneiEG11463. corA.

Phylogenomic databases

eggNOGiENOG4107QUT. Bacteria.
COG0598. LUCA.
HOGENOMiHOG000276873.
InParanoidiP0ABI4.
KOiK03284.
OMAiRRAVSFM.
OrthoDBiEOG6MD91T.
PhylomeDBiP0ABI4.

Enzyme and pathway databases

BioCyciEcoCyc:CORA-MONOMER.
ECOL316407:JW3789-MONOMER.
MetaCyc:CORA-MONOMER.

Miscellaneous databases

PROiP0ABI4.

Family and domain databases

InterProiIPR004488. Mg/Co-transport_prot_CorA.
IPR002523. MgTranspt_CorA/ZnTranspt_ZntB.
[Graphical view]
PfamiPF01544. CorA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00383. corA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and topology of the CorA magnesium transport systems of Salmonella typhimurium and Escherichia coli. Identification of a new class of transport protein."
    Smith R.L., Banks J.L., Snavely M.D., Maguire M.E.
    J. Biol. Chem. 268:14071-14080(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Physical map of the corA region of the E.coli chromosome."
    Ohmori H.
    Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology."
    Park M.H., Wong B.B., Lusk J.E.
    J. Bacteriol. 126:1096-1103(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCORA_ECOLI
AccessioniPrimary (citable) accession number: P0ABI4
Secondary accession number(s): P27841, Q2M8C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: April 13, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.