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Protein

ATP-dependent Clp protease ATP-binding subunit ClpA

Gene

clpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent specificity component of the ClpAP protease. It directs the protease to specific substrates. It has unfoldase activity. The primary function of the ClpA-ClpP complex appears to be the degradation of unfolded or abnormal proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi214 – 221ATPSequence analysis8
Nucleotide bindingi495 – 502ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: EcoCyc
  • ATP-dependent peptidase activity Source: CACAO

GO - Biological processi

  • protein unfolding Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10156-MONOMER.
ECOL316407:JW0866-MONOMER.
MetaCyc:EG10156-MONOMER.
SABIO-RKP0ABH9.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease ATP-binding subunit ClpA
Gene namesi
Name:clpA
Synonyms:lopD
Ordered Locus Names:b0882, JW0866
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10156. clpA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001910791 – 758ATP-dependent Clp protease ATP-binding subunit ClpAAdd BLAST758

Proteomic databases

EPDiP0ABH9.
PaxDbiP0ABH9.
PRIDEiP0ABH9.

Interactioni

Subunit structurei

Component of the ClpAP complex composed of six ClpA subunits assembled into a hexameric ring in the presence of ATP, and fourteen ClpP subunits arranged in two heptameric rings. Binds to ClpS.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
clpPP0A6G73EBI-546140,EBI-370625
clpSP0A8Q610EBI-546140,EBI-561456
fixXP686462EBI-546140,EBI-1113234

Protein-protein interaction databases

BioGridi4261320. 404 interactors.
850131. 1 interactor.
DIPiDIP-35409N.
IntActiP0ABH9. 75 interactors.
STRINGi511145.b0882.

Structurei

Secondary structure

1758
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Beta strandi22 – 25Combined sources4
Helixi27 – 34Combined sources8
Helixi38 – 46Combined sources9
Helixi51 – 65Combined sources15
Beta strandi72 – 74Combined sources3
Helixi82 – 96Combined sources15
Beta strandi97 – 99Combined sources3
Beta strandi101 – 103Combined sources3
Helixi105 – 112Combined sources8
Helixi119 – 126Combined sources8
Helixi131 – 139Combined sources9
Beta strandi171 – 173Combined sources3
Helixi176 – 181Combined sources6
Helixi192 – 202Combined sources11
Beta strandi204 – 207Combined sources4
Beta strandi209 – 213Combined sources5
Helixi220 – 233Combined sources14
Helixi238 – 240Combined sources3
Beta strandi244 – 247Combined sources4
Beta strandi259 – 261Combined sources3
Helixi263 – 274Combined sources12
Beta strandi275 – 278Combined sources4
Beta strandi280 – 284Combined sources5
Turni285 – 292Combined sources8
Beta strandi296 – 298Combined sources3
Helixi300 – 307Combined sources8
Beta strandi311 – 313Combined sources3
Beta strandi317 – 322Combined sources6
Helixi324 – 328Combined sources5
Helixi329 – 333Combined sources5
Helixi338 – 340Combined sources3
Beta strandi341 – 345Combined sources5
Helixi351 – 369Combined sources19
Helixi375 – 388Combined sources14
Helixi396 – 411Combined sources16
Beta strandi412 – 414Combined sources3
Helixi423 – 433Combined sources11
Beta strandi441 – 444Combined sources4
Helixi445 – 456Combined sources12
Turni457 – 459Combined sources3
Helixi464 – 478Combined sources15
Beta strandi484 – 486Combined sources3
Beta strandi488 – 494Combined sources7
Helixi501 – 512Combined sources12
Beta strandi515 – 520Combined sources6
Helixi521 – 523Combined sources3
Beta strandi525 – 528Combined sources4
Beta strandi531 – 533Combined sources3
Helixi541 – 545Combined sources5
Helixi548 – 555Combined sources8
Beta strandi557 – 564Combined sources8
Helixi566 – 568Combined sources3
Helixi571 – 583Combined sources13
Beta strandi584 – 588Combined sources5
Turni589 – 591Combined sources3
Beta strandi592 – 595Combined sources4
Beta strandi599 – 605Combined sources7
Helixi629 – 635Combined sources7
Helixi638 – 641Combined sources4
Beta strandi645 – 649Combined sources5
Helixi655 – 675Combined sources21
Beta strandi678 – 682Combined sources5
Helixi684 – 694Combined sources11
Turni697 – 699Combined sources3
Turni701 – 703Combined sources3
Helixi704 – 712Combined sources9
Helixi714 – 716Combined sources3
Helixi717 – 722Combined sources6
Turni724 – 727Combined sources4
Beta strandi729 – 736Combined sources8
Turni737 – 740Combined sources4
Beta strandi741 – 748Combined sources8
Beta strandi750 – 752Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K6KX-ray1.80A1-143[»]
1KSFX-ray2.60X1-758[»]
1LZWX-ray2.50B1-146[»]
1MBUX-ray2.30A/B1-142[»]
1MBVX-ray3.30A1-142[»]
1MBXX-ray2.25A/B1-142[»]
1MG9X-ray2.30B1-146[»]
1R6BX-ray2.25X1-758[»]
1R6CX-ray2.15X1-143[»]
1R6OX-ray2.25A/B1-143[»]
1R6QX-ray2.35A/B1-143[»]
ProteinModelPortaliP0ABH9.
SMRiP0ABH9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABH9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 417IAdd BLAST249
Regioni421 – 609IIAdd BLAST189

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218210.
InParanoidiP0ABH9.
KOiK03694.
OMAiDHSPDAM.
PhylomeDBiP0ABH9.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR013461. ClpA.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR02639. ClpA. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ABH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQELELSL NMAFARAREH RHEFMTVEHL LLALLSNPSA REALEACSVD
60 70 80 90 100
LVALRQELEA FIEQTTPVLP ASEEERDTQP TLSFQRVLQR AVFHVQSSGR
110 120 130 140 150
NEVTGANVLV AIFSEQESQA AYLLRKHEVS RLDVVNFISH GTRKDEPTQS
160 170 180 190 200
SDPGSQPNSE EQAGGEERME NFTTNLNQLA RVGGIDPLIG REKELERAIQ
210 220 230 240 250
VLCRRRKNNP LLVGESGVGK TAIAEGLAWR IVQGDVPEVM ADCTIYSLDI
260 270 280 290 300
GSLLAGTKYR GDFEKRFKAL LKQLEQDTNS ILFIDEIHTI IGAGAASGGQ
310 320 330 340 350
VDAANLIKPL LSSGKIRVIG STTYQEFSNI FEKDRALARR FQKIDITEPS
360 370 380 390 400
IEETVQIING LKPKYEAHHD VRYTAKAVRA AVELAVKYIN DRHLPDKAID
410 420 430 440 450
VIDEAGARAR LMPVSKRKKT VNVADIESVV ARIARIPEKS VSQSDRDTLK
460 470 480 490 500
NLGDRLKMLV FGQDKAIEAL TEAIKMARAG LGHEHKPVGS FLFAGPTGVG
510 520 530 540 550
KTEVTVQLSK ALGIELLRFD MSEYMERHTV SRLIGAPPGY VGFDQGGLLT
560 570 580 590 600
DAVIKHPHAV LLLDEIEKAH PDVFNILLQV MDNGTLTDNN GRKADFRNVV
610 620 630 640 650
LVMTTNAGVR ETERKSIGLI HQDNSTDAME EIKKIFTPEF RNRLDNIIWF
660 670 680 690 700
DHLSTDVIHQ VVDKFIVELQ VQLDQKGVSL EVSQEARNWL AEKGYDRAMG
710 720 730 740 750
ARPMARVIQD NLKKPLANEL LFGSLVDGGQ VTVALDKEKN ELTYGFQSAQ

KHKAEAAH
Length:758
Mass (Da):84,207
Last modified:October 25, 2005 - v1
Checksum:i0C96A2EB64A9F7DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti367A → G in AAA23583 (PubMed:2186030).Curated1
Sequence conflicti411L → V in AAA23583 (PubMed:2186030).Curated1
Sequence conflicti533L → V in AAA23583 (PubMed:2186030).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31045 Genomic DNA. Translation: AAA23583.1.
U00096 Genomic DNA. Translation: AAC73969.1.
AP009048 Genomic DNA. Translation: BAA35601.1.
M23220 Genomic DNA. Translation: AAA23582.1.
PIRiB64827. SUECCA.
RefSeqiNP_415403.1. NC_000913.3.
WP_000934041.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73969; AAC73969; b0882.
BAA35601; BAA35601; BAA35601.
GeneIDi945764.
KEGGiecj:JW0866.
eco:b0882.
PATRICi32116973. VBIEscCol129921_0912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31045 Genomic DNA. Translation: AAA23583.1.
U00096 Genomic DNA. Translation: AAC73969.1.
AP009048 Genomic DNA. Translation: BAA35601.1.
M23220 Genomic DNA. Translation: AAA23582.1.
PIRiB64827. SUECCA.
RefSeqiNP_415403.1. NC_000913.3.
WP_000934041.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K6KX-ray1.80A1-143[»]
1KSFX-ray2.60X1-758[»]
1LZWX-ray2.50B1-146[»]
1MBUX-ray2.30A/B1-142[»]
1MBVX-ray3.30A1-142[»]
1MBXX-ray2.25A/B1-142[»]
1MG9X-ray2.30B1-146[»]
1R6BX-ray2.25X1-758[»]
1R6CX-ray2.15X1-143[»]
1R6OX-ray2.25A/B1-143[»]
1R6QX-ray2.35A/B1-143[»]
ProteinModelPortaliP0ABH9.
SMRiP0ABH9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261320. 404 interactors.
850131. 1 interactor.
DIPiDIP-35409N.
IntActiP0ABH9. 75 interactors.
STRINGi511145.b0882.

Proteomic databases

EPDiP0ABH9.
PaxDbiP0ABH9.
PRIDEiP0ABH9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73969; AAC73969; b0882.
BAA35601; BAA35601; BAA35601.
GeneIDi945764.
KEGGiecj:JW0866.
eco:b0882.
PATRICi32116973. VBIEscCol129921_0912.

Organism-specific databases

EchoBASEiEB0154.
EcoGeneiEG10156. clpA.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218210.
InParanoidiP0ABH9.
KOiK03694.
OMAiDHSPDAM.
PhylomeDBiP0ABH9.

Enzyme and pathway databases

BioCyciEcoCyc:EG10156-MONOMER.
ECOL316407:JW0866-MONOMER.
MetaCyc:EG10156-MONOMER.
SABIO-RKP0ABH9.

Miscellaneous databases

EvolutionaryTraceiP0ABH9.
PROiP0ABH9.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR013461. ClpA.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR02639. ClpA. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPA_ECOLI
AccessioniPrimary (citable) accession number: P0ABH9
Secondary accession number(s): P15716, P77686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.