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P0ABH9

- CLPA_ECOLI

UniProt

P0ABH9 - CLPA_ECOLI

Protein

ATP-dependent Clp protease ATP-binding subunit ClpA

Gene

clpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    ATP-dependent specificity component of the ClpAP protease. It directs the protease to specific substrates. It has unfoldase activity. The primary function of the ClpA-ClpP complex appears to be the degradation of unfolded or abnormal proteins.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi214 – 2218ATPSequence Analysis
    Nucleotide bindingi495 – 5028ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent peptidase activity Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. proteolysis Source: EcoCyc
    2. response to oxidative stress Source: EcoCyc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10156-MONOMER.
    ECOL316407:JW0866-MONOMER.
    MetaCyc:EG10156-MONOMER.
    SABIO-RKP0ABH9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent Clp protease ATP-binding subunit ClpA
    Gene namesi
    Name:clpA
    Synonyms:lopD
    Ordered Locus Names:b0882, JW0866
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10156. clpA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 758758ATP-dependent Clp protease ATP-binding subunit ClpAPRO_0000191079Add
    BLAST

    Proteomic databases

    PaxDbiP0ABH9.
    PRIDEiP0ABH9.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ABH9.

    Interactioni

    Subunit structurei

    Component of the ClpAP complex composed of six ClpA subunits assembled into a hexameric ring in the presence of ATP, and fourteen ClpP subunits arranged in two heptameric rings. Binds to ClpS.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    clpSP0A8Q610EBI-546140,EBI-561456
    fixXP686462EBI-546140,EBI-1113234

    Protein-protein interaction databases

    BioGridi850131. 1 interaction.
    DIPiDIP-35409N.
    IntActiP0ABH9. 75 interactions.
    STRINGi511145.b0882.

    Structurei

    Secondary structure

    1
    758
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2017
    Beta strandi22 – 254
    Helixi27 – 348
    Helixi38 – 469
    Helixi51 – 6515
    Beta strandi72 – 743
    Helixi82 – 9615
    Beta strandi97 – 993
    Beta strandi101 – 1033
    Helixi105 – 1128
    Helixi119 – 1268
    Helixi131 – 1399
    Beta strandi171 – 1733
    Helixi176 – 1816
    Helixi192 – 20211
    Beta strandi204 – 2074
    Beta strandi209 – 2135
    Helixi220 – 23314
    Helixi238 – 2403
    Beta strandi244 – 2474
    Beta strandi259 – 2613
    Helixi263 – 27412
    Beta strandi275 – 2784
    Beta strandi280 – 2845
    Turni285 – 2928
    Beta strandi296 – 2983
    Helixi300 – 3078
    Beta strandi311 – 3133
    Beta strandi317 – 3226
    Helixi324 – 3285
    Helixi329 – 3335
    Helixi338 – 3403
    Beta strandi341 – 3455
    Helixi351 – 36919
    Helixi375 – 38814
    Helixi396 – 41116
    Beta strandi412 – 4143
    Helixi423 – 43311
    Beta strandi441 – 4444
    Helixi445 – 45612
    Turni457 – 4593
    Helixi464 – 47815
    Beta strandi484 – 4863
    Beta strandi488 – 4947
    Helixi501 – 51212
    Beta strandi515 – 5206
    Helixi521 – 5233
    Beta strandi525 – 5284
    Beta strandi531 – 5333
    Helixi541 – 5455
    Helixi548 – 5558
    Beta strandi557 – 5648
    Helixi566 – 5683
    Helixi571 – 58313
    Beta strandi584 – 5885
    Turni589 – 5913
    Beta strandi592 – 5954
    Beta strandi599 – 6057
    Helixi629 – 6357
    Helixi638 – 6414
    Beta strandi645 – 6495
    Helixi655 – 67521
    Beta strandi678 – 6825
    Helixi684 – 69411
    Turni697 – 6993
    Turni701 – 7033
    Helixi704 – 7129
    Helixi714 – 7163
    Helixi717 – 7226
    Turni724 – 7274
    Beta strandi729 – 7368
    Turni737 – 7404
    Beta strandi741 – 7488
    Beta strandi750 – 7523

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K6KX-ray1.80A1-143[»]
    1KSFX-ray2.60X1-758[»]
    1LZWX-ray2.50B1-146[»]
    1MBUX-ray2.30A/B1-142[»]
    1MBVX-ray3.30A1-142[»]
    1MBXX-ray2.25A/B1-142[»]
    1MG9X-ray2.30B1-146[»]
    1R6BX-ray2.25X1-758[»]
    1R6CX-ray2.15X1-143[»]
    1R6OX-ray2.25A/B1-143[»]
    1R6QX-ray2.35A/B1-143[»]
    ProteinModelPortaliP0ABH9.
    SMRiP0ABH9. Positions 1-755.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ABH9.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni169 – 417249IAdd
    BLAST
    Regioni421 – 609189IIAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ClpA/ClpB family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0542.
    HOGENOMiHOG000218210.
    KOiK03694.
    OMAiATDICAD.
    OrthoDBiEOG65F8SM.
    PhylomeDBiP0ABH9.

    Family and domain databases

    Gene3Di1.10.1780.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR019489. Clp_ATPase_C.
    IPR004176. Clp_N.
    IPR013461. ClpA.
    IPR001270. ClpA/B.
    IPR018368. ClpA/B_CS1.
    IPR028299. ClpA/B_CS2.
    IPR023150. Dbl_Clp-N.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11638:SF14. PTHR11638:SF14. 1 hit.
    PfamiPF00004. AAA. 1 hit.
    PF07724. AAA_2. 1 hit.
    PF02861. Clp_N. 1 hit.
    PF10431. ClpB_D2-small. 1 hit.
    [Graphical view]
    PRINTSiPR00300. CLPPROTEASEA.
    SMARTiSM00382. AAA. 2 hits.
    SM01086. ClpB_D2-small. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR02639. ClpA. 1 hit.
    PROSITEiPS00870. CLPAB_1. 1 hit.
    PS00871. CLPAB_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ABH9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNQELELSL NMAFARAREH RHEFMTVEHL LLALLSNPSA REALEACSVD    50
    LVALRQELEA FIEQTTPVLP ASEEERDTQP TLSFQRVLQR AVFHVQSSGR 100
    NEVTGANVLV AIFSEQESQA AYLLRKHEVS RLDVVNFISH GTRKDEPTQS 150
    SDPGSQPNSE EQAGGEERME NFTTNLNQLA RVGGIDPLIG REKELERAIQ 200
    VLCRRRKNNP LLVGESGVGK TAIAEGLAWR IVQGDVPEVM ADCTIYSLDI 250
    GSLLAGTKYR GDFEKRFKAL LKQLEQDTNS ILFIDEIHTI IGAGAASGGQ 300
    VDAANLIKPL LSSGKIRVIG STTYQEFSNI FEKDRALARR FQKIDITEPS 350
    IEETVQIING LKPKYEAHHD VRYTAKAVRA AVELAVKYIN DRHLPDKAID 400
    VIDEAGARAR LMPVSKRKKT VNVADIESVV ARIARIPEKS VSQSDRDTLK 450
    NLGDRLKMLV FGQDKAIEAL TEAIKMARAG LGHEHKPVGS FLFAGPTGVG 500
    KTEVTVQLSK ALGIELLRFD MSEYMERHTV SRLIGAPPGY VGFDQGGLLT 550
    DAVIKHPHAV LLLDEIEKAH PDVFNILLQV MDNGTLTDNN GRKADFRNVV 600
    LVMTTNAGVR ETERKSIGLI HQDNSTDAME EIKKIFTPEF RNRLDNIIWF 650
    DHLSTDVIHQ VVDKFIVELQ VQLDQKGVSL EVSQEARNWL AEKGYDRAMG 700
    ARPMARVIQD NLKKPLANEL LFGSLVDGGQ VTVALDKEKN ELTYGFQSAQ 750
    KHKAEAAH 758
    Length:758
    Mass (Da):84,207
    Last modified:October 25, 2005 - v1
    Checksum:i0C96A2EB64A9F7DC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti367 – 3671A → G in AAA23583. (PubMed:2186030)Curated
    Sequence conflicti411 – 4111L → V in AAA23583. (PubMed:2186030)Curated
    Sequence conflicti533 – 5331L → V in AAA23583. (PubMed:2186030)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31045 Genomic DNA. Translation: AAA23583.1.
    U00096 Genomic DNA. Translation: AAC73969.1.
    AP009048 Genomic DNA. Translation: BAA35601.1.
    M23220 Genomic DNA. Translation: AAA23582.1.
    PIRiB64827. SUECCA.
    RefSeqiNP_415403.1. NC_000913.3.
    YP_489155.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73969; AAC73969; b0882.
    BAA35601; BAA35601; BAA35601.
    GeneIDi12932326.
    945764.
    KEGGiecj:Y75_p0855.
    eco:b0882.
    PATRICi32116973. VBIEscCol129921_0912.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31045 Genomic DNA. Translation: AAA23583.1 .
    U00096 Genomic DNA. Translation: AAC73969.1 .
    AP009048 Genomic DNA. Translation: BAA35601.1 .
    M23220 Genomic DNA. Translation: AAA23582.1 .
    PIRi B64827. SUECCA.
    RefSeqi NP_415403.1. NC_000913.3.
    YP_489155.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K6K X-ray 1.80 A 1-143 [» ]
    1KSF X-ray 2.60 X 1-758 [» ]
    1LZW X-ray 2.50 B 1-146 [» ]
    1MBU X-ray 2.30 A/B 1-142 [» ]
    1MBV X-ray 3.30 A 1-142 [» ]
    1MBX X-ray 2.25 A/B 1-142 [» ]
    1MG9 X-ray 2.30 B 1-146 [» ]
    1R6B X-ray 2.25 X 1-758 [» ]
    1R6C X-ray 2.15 X 1-143 [» ]
    1R6O X-ray 2.25 A/B 1-143 [» ]
    1R6Q X-ray 2.35 A/B 1-143 [» ]
    ProteinModelPortali P0ABH9.
    SMRi P0ABH9. Positions 1-755.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 850131. 1 interaction.
    DIPi DIP-35409N.
    IntActi P0ABH9. 75 interactions.
    STRINGi 511145.b0882.

    Proteomic databases

    PaxDbi P0ABH9.
    PRIDEi P0ABH9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73969 ; AAC73969 ; b0882 .
    BAA35601 ; BAA35601 ; BAA35601 .
    GeneIDi 12932326.
    945764.
    KEGGi ecj:Y75_p0855.
    eco:b0882.
    PATRICi 32116973. VBIEscCol129921_0912.

    Organism-specific databases

    EchoBASEi EB0154.
    EcoGenei EG10156. clpA.

    Phylogenomic databases

    eggNOGi COG0542.
    HOGENOMi HOG000218210.
    KOi K03694.
    OMAi ATDICAD.
    OrthoDBi EOG65F8SM.
    PhylomeDBi P0ABH9.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10156-MONOMER.
    ECOL316407:JW0866-MONOMER.
    MetaCyc:EG10156-MONOMER.
    SABIO-RK P0ABH9.

    Miscellaneous databases

    EvolutionaryTracei P0ABH9.
    PROi P0ABH9.

    Gene expression databases

    Genevestigatori P0ABH9.

    Family and domain databases

    Gene3Di 1.10.1780.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR019489. Clp_ATPase_C.
    IPR004176. Clp_N.
    IPR013461. ClpA.
    IPR001270. ClpA/B.
    IPR018368. ClpA/B_CS1.
    IPR028299. ClpA/B_CS2.
    IPR023150. Dbl_Clp-N.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11638:SF14. PTHR11638:SF14. 1 hit.
    Pfami PF00004. AAA. 1 hit.
    PF07724. AAA_2. 1 hit.
    PF02861. Clp_N. 1 hit.
    PF10431. ClpB_D2-small. 1 hit.
    [Graphical view ]
    PRINTSi PR00300. CLPPROTEASEA.
    SMARTi SM00382. AAA. 2 hits.
    SM01086. ClpB_D2-small. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR02639. ClpA. 1 hit.
    PROSITEi PS00870. CLPAB_1. 1 hit.
    PS00871. CLPAB_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate."
      Gottesman S., Clark W.P., Maurizi M.R.
      J. Biol. Chem. 265:7886-7893(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The two-component, ATP-dependent Clp protease of Escherichia coli. Purification, cloning, and mutational analysis of the ATP-binding component."
      Katayama Y., Gottesman S., Pumphrey J., Rudikoff S., Clark W.P., Maurizi M.R.
      J. Biol. Chem. 263:15226-15236(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28, PARTIAL PROTEIN SEQUENCE.
      Strain: SG1110.
    6. "Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate."
      Cummings H.S., Sands J.F., Foreman P.C., Fraser J., Hershey J.W.B.
      J. Biol. Chem. 266:16491-16498(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 511-758.
    7. Cited for: SIMILARITY TO OTHER CLPAB LIKE PROTEINS.
    8. "Crystal structure of ClpA, an Hsp100 chaperone and regulator of ClpAP protease."
      Guo F., Maurizi M.R., Esser L., Xia D.
      J. Biol. Chem. 277:46743-46752(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 1-143.
    9. "Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA."
      Zeth K., Ravelli R.B., Paal K., Cusack S., Bukau B., Dougan D.A.
      Nat. Struct. Biol. 9:906-911(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-146 IN COMPLEX WITH CLPS.
    10. "Crystal structure of the heterodimeric complex of the adaptor, ClpS, with the N-domain of the AAA+ chaperone, ClpA."
      Guo F., Esser L., Singh S.K., Maurizi M.R., Xia D.
      J. Biol. Chem. 277:46753-46762(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-143 IN COMPLEX WITH CLPS.

    Entry informationi

    Entry nameiCLPA_ECOLI
    AccessioniPrimary (citable) accession number: P0ABH9
    Secondary accession number(s): P15716, P77686
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3