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Protein

Peptidoglycan glycosyltransferase MrdB

Gene

mrdB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Peptidoglycan polymerase that is essential for cell wall elongation (PubMed:27643381). Also required for the maintenance of the rod cell shape (PubMed:2644207). Functions probably in conjunction with the penicillin-binding protein 2 (mrdA) (PubMed:2644207, PubMed:27643381).2 Publications

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.UniRule annotation1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: InterPro
  • cell division Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: UniProtKB-KW
  • regulation of cell shape Source: EcoliWiki

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10607-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

TCDBi2.A.103.1.2. the bacterial murein precursor exporter (mpe) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan glycosyltransferase MrdBUniRule annotationCurated (EC:2.4.1.129UniRule annotation1 Publication)
Short name:
PGTUniRule annotationCurated
Alternative name(s):
Cell elongation protein RodAUniRule annotationCurated
Cell wall polymeraseUniRule annotationCurated
Peptidoglycan polymerase1 PublicationUniRule annotation
Short name:
PG polymerase1 PublicationUniRule annotation
Rod shape-determining proteinCurated
Gene namesi
Name:mrdB1 PublicationUniRule annotation
Synonyms:rodA1 Publication
Ordered Locus Names:b0634, JW0629
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10607. mrdB.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19PeriplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
Topological domaini41 – 49CytoplasmicSequence analysis9
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 74PeriplasmicSequence analysis4
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 135CytoplasmicSequence analysisAdd BLAST40
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 159PeriplasmicSequence analysis3
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 182CytoplasmicSequence analysis2
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 262PeriplasmicSequence analysisAdd BLAST59
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 311CytoplasmicSequence analysisAdd BLAST28
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 335PeriplasmicSequence analysis3
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Topological domaini357 – 370CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

At 42 degrees Celsius, mutation causes formation of spherical cells and mecillinam resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000627151 – 370Peptidoglycan glycosyltransferase MrdBAdd BLAST370

Proteomic databases

PaxDbiP0ABG7.
PRIDEiP0ABG7.

Interactioni

Protein-protein interaction databases

BioGridi4259910. 253 interactors.
DIPiDIP-48062N.
IntActiP0ABG7. 1 interactor.
MINTiMINT-1235519.
STRINGi511145.b0634.

Structurei

3D structure databases

ProteinModelPortaliP0ABG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SEDS family. MrdB/RodA subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNI. Bacteria.
COG0772. LUCA.
HOGENOMiHOG000282686.
InParanoidiP0ABG7.
KOiK05837.
OMAiATIAWYL.
PhylomeDBiP0ABG7.

Family and domain databases

HAMAPiMF_02079. PGT_RodA. 1 hit.
InterProiView protein in InterPro
IPR018365. Cell_cycle_FtsW-rel_CS.
IPR001182. Cell_cycle_FtsW/RodA.
IPR011923. RodA_shape.
PANTHERiPTHR30474. PTHR30474. 1 hit.
PTHR30474:SF6. PTHR30474:SF6. 1 hit.
PfamiView protein in Pfam
PF01098. FTSW_RODA_SPOVE. 1 hit.
TIGRFAMsiTIGR02210. rodA_shape. 1 hit.
PROSITEiView protein in PROSITE
PS00428. FTSW_RODA_SPOVE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNPNKKTF WDKVHLDPTM LLILLALLVY SALVIWSASG QDIGMMERKI
60 70 80 90 100
GQIAMGLVIM VVMAQIPPRV YEGWAPYLYI ICIILLVAVD AFGAISKGAQ
110 120 130 140 150
RWLDLGIVRF QPSEIAKIAV PLMVARFINR DVCPPSLKNT GIALVLIFMP
160 170 180 190 200
TLLVAAQPDL GTSILVALSG LFVLFLSGLS WRLIGVAVVL VAAFIPILWF
210 220 230 240 250
FLMHDYQRQR VMMLLDPESD PLGAGYHIIQ SKIAIGSGGL RGKGWLHGTQ
260 270 280 290 300
SQLEFLPERH TDFIFAVLAE ELGLVGILIL LALYILLIMR GLWIAARAQT
310 320 330 340 350
TFGRVMAGGL MLILFVYVFV NIGMVSGILP VVGVPLPLVS YGGSALIVLM
360 370
AGFGIVMSIH THRKMLSKSV
Length:370
Mass (Da):40,476
Last modified:October 25, 2005 - v1
Checksum:i68FCDF8D6B123D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22857 Genomic DNA. Translation: AAA24571.1.
U82598 Genomic DNA. Translation: AAB40834.1.
U00096 Genomic DNA. Translation: AAC73735.1.
AP009048 Genomic DNA. Translation: BAA35277.1.
M18276 Genomic DNA. Translation: AAA24551.1.
PIRiJT0500. BVECRD.
RefSeqiNP_415167.1. NC_000913.3.
WP_000131719.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73735; AAC73735; b0634.
BAA35277; BAA35277; BAA35277.
GeneIDi945238.
KEGGiecj:JW0629.
eco:b0634.
PATRICi32116451. VBIEscCol129921_0665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22857 Genomic DNA. Translation: AAA24571.1.
U82598 Genomic DNA. Translation: AAB40834.1.
U00096 Genomic DNA. Translation: AAC73735.1.
AP009048 Genomic DNA. Translation: BAA35277.1.
M18276 Genomic DNA. Translation: AAA24551.1.
PIRiJT0500. BVECRD.
RefSeqiNP_415167.1. NC_000913.3.
WP_000131719.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259910. 253 interactors.
DIPiDIP-48062N.
IntActiP0ABG7. 1 interactor.
MINTiMINT-1235519.
STRINGi511145.b0634.

Protein family/group databases

TCDBi2.A.103.1.2. the bacterial murein precursor exporter (mpe) family.

Proteomic databases

PaxDbiP0ABG7.
PRIDEiP0ABG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73735; AAC73735; b0634.
BAA35277; BAA35277; BAA35277.
GeneIDi945238.
KEGGiecj:JW0629.
eco:b0634.
PATRICi32116451. VBIEscCol129921_0665.

Organism-specific databases

EchoBASEiEB0602.
EcoGeneiEG10607. mrdB.

Phylogenomic databases

eggNOGiENOG4105CNI. Bacteria.
COG0772. LUCA.
HOGENOMiHOG000282686.
InParanoidiP0ABG7.
KOiK05837.
OMAiATIAWYL.
PhylomeDBiP0ABG7.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:EG10607-MONOMER.

Miscellaneous databases

PROiP0ABG7.

Family and domain databases

HAMAPiMF_02079. PGT_RodA. 1 hit.
InterProiView protein in InterPro
IPR018365. Cell_cycle_FtsW-rel_CS.
IPR001182. Cell_cycle_FtsW/RodA.
IPR011923. RodA_shape.
PANTHERiPTHR30474. PTHR30474. 1 hit.
PTHR30474:SF6. PTHR30474:SF6. 1 hit.
PfamiView protein in Pfam
PF01098. FTSW_RODA_SPOVE. 1 hit.
TIGRFAMsiTIGR02210. rodA_shape. 1 hit.
PROSITEiView protein in PROSITE
PS00428. FTSW_RODA_SPOVE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRODA_ECOLI
AccessioniPrimary (citable) accession number: P0ABG7
Secondary accession number(s): P13409, P15035
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: March 15, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.