Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptidoglycan glycosyltransferase MrdB

Gene

mrdB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Peptidoglycan polymerase that is essential for cell wall elongation (PubMed:27643381). Also required for the maintenance of the rod cell shape (PubMed:2644207). Functions probably in conjunction with the penicillin-binding protein 2 (mrdA) (PubMed:2644207, PubMed:27643381).2 Publications

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.UniRule annotation1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: InterPro
  • cell division Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  • regulation of cell shape Source: EcoliWiki

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10607-MONOMER
UniPathwayiUPA00219

Protein family/group databases

TCDBi2.A.103.1.2 the bacterial murein precursor exporter (mpe) family

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan glycosyltransferase MrdBUniRule annotationCurated (EC:2.4.1.129UniRule annotation1 Publication)
Short name:
PGTUniRule annotationCurated
Alternative name(s):
Cell elongation protein RodAUniRule annotationCurated
Cell wall polymeraseUniRule annotationCurated
Peptidoglycan polymerase1 PublicationUniRule annotation
Short name:
PG polymerase1 PublicationUniRule annotation
Rod shape-determining proteinCurated
Gene namesi
Name:mrdB1 PublicationUniRule annotation
Synonyms:rodA1 Publication
Ordered Locus Names:b0634, JW0629
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10607 mrdB

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19PeriplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
Topological domaini41 – 49CytoplasmicSequence analysis9
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 74PeriplasmicSequence analysis4
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 135CytoplasmicSequence analysisAdd BLAST40
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 159PeriplasmicSequence analysis3
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 182CytoplasmicSequence analysis2
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 262PeriplasmicSequence analysisAdd BLAST59
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 311CytoplasmicSequence analysisAdd BLAST28
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 335PeriplasmicSequence analysis3
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Topological domaini357 – 370CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

At 42 degrees Celsius, mutation causes formation of spherical cells and mecillinam resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000627151 – 370Peptidoglycan glycosyltransferase MrdBAdd BLAST370

Proteomic databases

PaxDbiP0ABG7
PRIDEiP0ABG7

Interactioni

Protein-protein interaction databases

BioGridi4259910, 288 interactors
DIPiDIP-48062N
IntActiP0ABG7, 1 interactor
STRINGi316385.ECDH10B_0703

Structurei

3D structure databases

ProteinModelPortaliP0ABG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SEDS family. MrdB/RodA subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNI Bacteria
COG0772 LUCA
HOGENOMiHOG000282686
InParanoidiP0ABG7
KOiK05837
OMAiHDYQKKR
PhylomeDBiP0ABG7

Family and domain databases

HAMAPiMF_02079 PGT_RodA, 1 hit
InterProiView protein in InterPro
IPR018365 Cell_cycle_FtsW-rel_CS
IPR001182 FtsW/RodA
IPR011923 RodA/MrdB
PANTHERiPTHR30474 PTHR30474, 1 hit
PTHR30474:SF1 PTHR30474:SF1, 1 hit
PfamiView protein in Pfam
PF01098 FTSW_RODA_SPOVE, 1 hit
TIGRFAMsiTIGR02210 rodA_shape, 1 hit
PROSITEiView protein in PROSITE
PS00428 FTSW_RODA_SPOVE, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNPNKKTF WDKVHLDPTM LLILLALLVY SALVIWSASG QDIGMMERKI
60 70 80 90 100
GQIAMGLVIM VVMAQIPPRV YEGWAPYLYI ICIILLVAVD AFGAISKGAQ
110 120 130 140 150
RWLDLGIVRF QPSEIAKIAV PLMVARFINR DVCPPSLKNT GIALVLIFMP
160 170 180 190 200
TLLVAAQPDL GTSILVALSG LFVLFLSGLS WRLIGVAVVL VAAFIPILWF
210 220 230 240 250
FLMHDYQRQR VMMLLDPESD PLGAGYHIIQ SKIAIGSGGL RGKGWLHGTQ
260 270 280 290 300
SQLEFLPERH TDFIFAVLAE ELGLVGILIL LALYILLIMR GLWIAARAQT
310 320 330 340 350
TFGRVMAGGL MLILFVYVFV NIGMVSGILP VVGVPLPLVS YGGSALIVLM
360 370
AGFGIVMSIH THRKMLSKSV
Length:370
Mass (Da):40,476
Last modified:October 25, 2005 - v1
Checksum:i68FCDF8D6B123D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22857 Genomic DNA Translation: AAA24571.1
U82598 Genomic DNA Translation: AAB40834.1
U00096 Genomic DNA Translation: AAC73735.1
AP009048 Genomic DNA Translation: BAA35277.1
M18276 Genomic DNA Translation: AAA24551.1
PIRiJT0500 BVECRD
RefSeqiNP_415167.1, NC_000913.3
WP_000131719.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73735; AAC73735; b0634
BAA35277; BAA35277; BAA35277
GeneIDi945238
KEGGiecj:JW0629
eco:b0634
PATRICifig|1411691.4.peg.1634

Similar proteinsi

Entry informationi

Entry nameiRODA_ECOLI
AccessioniPrimary (citable) accession number: P0ABG7
Secondary accession number(s): P13409, P15035
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health