Reviewed, UniProtKB/Swiss-Prot P0ABG3 (CDSA_SHIFL)

Names and origin

Protein names

Recommended name:
    Phosphatidate cytidylyltransferase
    EC=2.7.7.41
Alternative name(s):
    CDP-diglyceride pyrophosphorylase
    CDP-diglyceride synthase
    CDP-diacylglycerol synthase
      Short name=CDS
    CTP:phosphatidate cytidylyltransferase
    CDP-DG synthase
    CDP-DAG synthase

Gene names

Name:	cdsA
Ordered Locus Names:	SF0165, S0168

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the CDS family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphatidate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Phosphatidate cytidylyltransferase		PRO_0000090746
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	56 – 76	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	121 – 141	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	264 – 284	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ABG3-1 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 0D77DCD5EC5FBA9F	FASTA	285	31,454
	10 20 30 40 50 60 MLKYRLISAF VLIPVVIAAL FLLPPVGFAI VTLVVCMLAA WEWGQLSGFT TRSQRVWLAV 70 80 90 100 110 120 LCGLLLALML FLLPEYHRNI HQPLVEISLW ASLGWWIVAL LLVLFYPGSA AIWRNSKTLR 130 140 150 160 170 180 LIFGVLTIVP FFWGMLALRA WHYDENHYSG AIWLLYVMIL VWGADSGAYM FGKLFGKHKL 190 200 210 220 230 240 APKVSPGKTW QGFIGGLATA AVISWGYGMW ANLDVAPVTL LICSIVAALA SVLGDLTESM 250 260 270 280 FKREAGIKDS GHLIPGHGGI LDRIDSLTAA VPVFACLLLL VFRTL

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN41827.1. Different initiation. AE014073 Genomic DNA. Translation: AAP15708.1. Different initiation.
RefSeq	NP_706120.3. NP_835903.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1024478. 1076599.
GenomeReviews	Gene locus SF0165 in contig AE005674_GR. Gene locus S0168 in contig AE014073_GR.
KEGG	sfl:SF0165. sfx:S0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732679.
OMA	WPLGNWL.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN41827.1-MONOMER.
BRENDA	2.7.7.41. 189495.
Family and domain databases
InterPro	IPR000374. PC_trans. [Graphical view]
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDSA_SHIFL

Accession

Primary (citable) accession number: P0ABG3
Secondary accession number(s): P06466

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)