P0ABG1 (CDSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidate cytidylyltransferase
EC=2.7.7.41

Alternative name(s):
CDP-DAG synthase
CDP-DG synthase
CDP-diacylglycerol synthase
Short name=CDS
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
CTP:phosphatidate cytidylyltransferase

Gene names

Name:	cdsA
Synonyms:	cds
Ordered Locus Names:	b0175, JW5810

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDS family.
Sequence caution	The sequence AAA23545.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB08604.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Phosphatidate cytidylyltransferase		PRO_0000090734
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	190 – 210	21	Helical; Potential
	Transmembrane	213 – 233	21	Helical; Potential
	Transmembrane	264 – 284	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ABG1 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 0D77DCD5EC5FBA9F	FASTA	285	31,454
	10 20 30 40 50 60 MLKYRLISAF VLIPVVIAAL FLLPPVGFAI VTLVVCMLAA WEWGQLSGFT TRSQRVWLAV 70 80 90 100 110 120 LCGLLLALML FLLPEYHRNI HQPLVEISLW ASLGWWIVAL LLVLFYPGSA AIWRNSKTLR 130 140 150 160 170 180 LIFGVLTIVP FFWGMLALRA WHYDENHYSG AIWLLYVMIL VWGADSGAYM FGKLFGKHKL 190 200 210 220 230 240 APKVSPGKTW QGFIGGLATA AVISWGYGMW ANLDVAPVTL LICSIVAALA SVLGDLTESM 250 260 270 280 FKREAGIKDS GHLIPGHGGI LDRIDSLTAA VPVFACLLLL VFRTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of the gene for CDP-diglyceride synthetase of Escherichia coli." Icho T., Sparrow C.P., Raetz C.R.H. J. Biol. Chem. 260:12078-12083(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli." Sparrow C.P., Raetz C.R.H. J. Biol. Chem. 260:12084-12091(1985) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11330 Genomic DNA. Translation: AAA23545.1. Different initiation. U70214 Genomic DNA. Translation: AAB08604.1. Different initiation. U00096 Genomic DNA. Translation: AAC73286.2. AP009048 Genomic DNA. Translation: BAA77850.2.
PIR	SYECDG. A23898.
RefSeq	NP_414717.2. NC_000913.2. YP_488477.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ABG1.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0175.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73286; AAC73286; b0175. BAA77850; BAA77850; BAA77850.
GeneID	12932204. 944876.
KEGG	ecj:Y75_p0171. eco:b0175.
PATRIC	32115461. VBIEscCol129921_0182.
Organism-specific databases
EchoBASE	EB0137.
EcoGene	EG10139. cdsA.
Phylogenomic databases
eggNOG	COG0575.
HOGENOM	HOG000006168.
KO	K00981.
OMA	YVFILVW.
ProtClustDB	PRK11624.
Enzyme and pathway databases
BioCyc	EcoCyc:CDPDIGLYSYN-MONOMER. ECOL316407:JW5810-MONOMER. MetaCyc:CDPDIGLYSYN-MONOMER.
UniPathway	UPA00557; UER00614.
Gene expression databases
Genevestigator	P0ABG1.
Family and domain databases
InterPro	IPR000374. PC_trans. [Graphical view]
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDSA_ECOLI

Accession

Primary (citable) accession number: P0ABG1
Secondary accession number(s): P06466

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program